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Biomedical subjects

R L Taylor

Publications and source records attributed to R L Taylor.

At least 145 records · Page 8Linked to original sources

Categorizing behavior in terms of severity: considerations for part two of Adaptive Behavior Scale.

Forty-five respondents were asked to rate the items in the Violent and Destructive Behavior domain from Part Two of the AAMD Adaptive Behavior Scale on a 1 (not very severe) to 4 (extremely severe) scale. In addition, they were asked to assign the items to one of five categories based on the effects of the behavior on the environment. There was considerable agreement concerning both the severity and the appropriate category for each item. Suggestions for revision of Part Two, including the need for a weighting system and more precise definitions of the behavior items, were discussed.

Humans↗

Purification of antibiotics from Physarum gyrosum by high pressure liquid chromatography.

A family of five antibiotic substances was isolated from the slime mold Physarum gyrosum by high pressure liquid chromatography (HPLC). For this purpose, mold was cultured for two weeks in a liquid medium. Soluble products were harvested by rotary evaporation of medium and extraction with 1-butanol. Paper chromatography in ethyl acetate:pyridine:water (2:2:1 v/v) was used for preliminary fractionation. Active components were separated by HPLC with a reverse-phase column packed with Bondapack C18/Porasil B (Waters Associates) and were eluted with a linear gradient of methanol:water increasing from 70 to 100% methanol over 90 minutes. Purification was completed by rechromatographing individual fractions. Purity of the active components was verified by HPLC and thin layer chromatography. Activity assays against Bacillus cereus showed these materials to be bacteriostatic rather than bacteriocidal.

Anti-Bacterial Agents↗

Transmission of salmonid whirling disease by birds fed trout infected with Myxosoma cerebralis.

Mallard ducks, Anas platyrhynchos and a black crested night heron, Nycticorax nycticorax were fed trout infected with Myxosoma cerebralis (HOFER) in 2 separate experiments. Feces from the birds were deposited in troughs containing M. cerebralis-free mud as well as in 1 trough without mud. Spore suspensions were also added directly to mud in 1 trough and to another without mud. Susceptible rainbow trout, Salmo gairdneri, developed whirling disease in all troughs containing mud contaminated with M. cerebralis but remained free of infection when exposed to M. cerebralis in troughs without mud. This demonstrates the possibility of bird transmission of the organism causing whirling disease to previously non-contaminated waters.

Animals↗

Host defense in cryptococcosis. I. An in vivo model for evaluating immune response.

An inbred mouse model was used to evaluate in vivo host immune response to Cryptococcus neoformans. Within 1 week of immunization, mice developed delayed type hypersensitivity reactions (DTH) to cryptococcal extracts derived from either culture filtrates or disrupted cells. There was no significant cross reactivity with extracts of other fungi. Previous immunization provided considerable protection against subsequent challenge with multiple strains of cryptococci. DTH also developed after nonimmunized mice were challenged with C. neoformans; however, in this situation DTH was not associated with prolonged survival. These studies indicate that mice can be immunized and protected against cryptococcosis and that this protection is associated with acquisition of DTH to cryptococcal antigens.

Animals↗

Transperitoneal relocation of the ileal stoma without formal laparotomy.

A method of transperitoneal ileal stoma relocation without formal laparotomy is described. Twenty of these procedures were performed during a ten year period. There was one postoperative failure. The barium stomal injection is a valuable aid in the peroperative diagnosis of possible underlying Crohn's disease. Transperitoneal relocation of an ileal stoma without formal laparotomy is a safe and useful method in selected patients.

Adolescent↗

Hepatic necrobacillosis. Report of a case resembling metastatic tumor.

A 47-year-old man had multiple pyogenic liver abscesses from which the anaerobic bacteria Fusobacterium nucleatum and Peptostreptococcus were isolated. The clinical course, diagnostic evaluation, and appearance of the lesions at surgery suggested metastatic tumor. Histologically, the lesions consisted of necrotic liver tissue surrounded by zones of acute and chronic inflammation and fibrous connective tissue closely resembling the lesions of hepatic necrobacillosis attributable to F necrophorum. Treatment with high doses of penicillin was curative.

Bacterial Infections↗

Scanning electron microscopy of human dental pellicle and initial plaque formation in vivo.

Small, specially prepared gold alloy discs were recessed into the right and left posterior surfaces of duplicate maxillary dentures. At selected time intervals, a disc was removed from each side of the denture and prepared for examination with a scanning electron microscope (SEM). A thin, peeling film of pellicle was seen on the discs removed after two hours and projections designated "hemispherical globules" formed on the pellicle within the next seven hours.

Bacteria↗

Sensory interaction: vision is modulated by hearing.

An irrelevant noise was turned on at various points during a visual reaction-time task. Latencies were reliably smaller than on quiet trials, provided the noise was turned on when a subject was looking at a relevant visual stimulus. This effect occurred regardless of whether the distribution of interstimulus intervals was constant or varied during a session, as well as independently of a subject's base rate of response and level of practice in the task. But it disappeared if reaction times in completely noisy sessions were compared to performances in quiet stimulated by a test form, but not with the particular ear stimulated by a noise. A model is suggested for these and related results in which the arousal elicited by a loud sound in the central nervous system either activates associative memories of form, inhibits early components of striate cortical unit response to the intensive properties of a visual stimulus, or both.

Auditory Perception↗

Sparganosis in the United States. Report of a case.

A 20-year-old Philadelphia woman (recently moved from South Carolina) had sparganosis of her lower anterior abdominal wall. In the United States sparganosis occurs when man enters the life cycle of dog or cat tapeworms of the genus Spirometra. Most of the American cases have been reported from the southeastern United States, and the most probable source of infection is contaminated drinking water. Sporadic cases have also been seen in other parts of the country. If the pathologist is familiar with the morphology of spargana, the gross and histologic features are sufficient for identification of genus Spirometra.

Adult↗

Overlay dentures. Philosophy and practice. II.

The practical stages in the construction of mandibular overlay dentures, with and without precision attachment anchors, are described and illustrated in detail. The importance of post-insertion maintenance, continuing effective oral hygiene and patient care, as a prerequisite for a successful overlay denture experience, is emphasized.

Dental Abutments↗

Psychosocial development among black children and youth: a reexamination.

The fundamental assumptions and empirical evidence, upon which are based conventional views of the nature and meaning of black self-esteem, are examined. Review of the literature reveals that a number of these assumptions and conclusions have been drawn from rather weak empirical data. Results of recent investigations, suggesting alternative interpretations and conclusions regarding the level and quality of black self-esteem, are presented.

Adolescent↗

Growth of Physarum gyrosum on agar plates and in liquid culture.

The physical and nutritional requirements of the antibiotic-producing slime mold Physarum gyrosum were examined to develop a liquid medium for this myxomycete. Liquid culture is desired to expedite a useful scale of production of antibiotic materials for ease of isolation and structure study. Culture conditions were selected to favor antibiotic production rather than maximum growth. The medium devised consisted of 0.010 M potassium phosphate buffer (pH 6.0), 2% bakers' yeast, and 0.2% glucose and was supplemented with either 10(-7) M hemoglobin (preferred) or 2.0 ml of live Escherichia coli per 100 ml of culture medium grown to a steady-state population in nutrient broth. The slime mold, which contained some E. coli carried along with the inoculum, was allowed to grow as a surface plasmodium at 20 degrees C in the dark with weekly subculturing for stocks or for 10 days for antibiotic production. P. gyrosum produced the same antibiotic materials when grown in liquid medium as it did when grown on agar plates. A seeded plate disk assay against Bacillus cereus was employed to follow antibiotic activity.

Anti-Bacterial Agents↗