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R Lalić

Publications and source records attributed to R Lalić.

11 recordsLinked to original sources
PubMed1993

[Modern immunologic tests in the diagnosis of Lyme borreliosis].

In this work we described the results that were obtained using various immunodiagnostic assays for the detection of lyme borreliosis. Sera of the patients that were in acute or chronical phase of the disease were analysed in indirect immunofluorescent, immunoenzyme and immunoblot assays which were prepared and carried out in our laboratory. As a control for the validity of these investigations, we used sera of the healthy people, as well as of the patients suffering of lues or rheumatoid illnesses. Results that we obtained pointed out the factors responsible for the nonspecific reactions in indirect immunofluorescent and immunoenzyme test. The advantage of the immunoblot analysis in detecting lyme borreliosis is described in this work.

Humans↗
PubMedDate not supplied

[Conditions required for chemical binding of Dactylis glomerata grass pollen to inert stable carriers].

The results of the direct RAST (Radio-Allergo-Sorbent-Test) procedure used to standardise own-prepared pollen extract of the Dactylis glomerata grass are presented. Based on the performed standardisation, a system was set up for binding allergens to the stable inert carriers. The results of examination of the quality of D. glomerata allergen, bound covalently to paper disc, are presented also. Evaluation was made in INEP laboratories by comparing the D. glomerata allergen-bound discs and the corresponding discs of Phadebas, Pharmacia Uppsala, confirming a high degree of correlation in the direct RAST procedure.

Poaceae↗
PubMedDate not supplied

[Use of the ELISA immunoenzyme test and indirect immunofluorescence in the diagnosis of a toxoplasmosis epidemic].

Etiologic agent of lymphadenitis outbreak in one our military environment was detected by two serologic tests: ELISA (enzyme-linked immunosorbent assay) and indirect immunofluorescence test (IIF) both specific for IgM and IgG antibody detection in toxoplasmosis diagnosis. IgM-ELISA analysis of the first serum sample taken from a group of 79 soldiers with lymphadenitis was positive in 65 examinees (82.28%) and IgG-IIF technique in 52 (65.82%). In a group of 33 soldiers from the same unit with no signs of the disease IgM-ELISA test was positive in 12 (36.36%) and IgM-IIF in 9 (27.27%). In a group of 40 officers from the unit with the disease outbreak specific IgM antibodies were found by both tests only in the head cook. IgM antibodies were not found in a control group of 102 healthy soldiers from other units of the same garrison. Results of this investigation thermal injury showed variations in immune response to infection in dependence on trauma effect duration (in dependence on the phase). In the early posttraumatic phase organism is capable to react effectively to the inoculated infective agent and in the late posttraumatic phase this reaction is much less effective. Authors conclude that these results may influence upon the thermal injury management strategy in dependence on the trauma effect duration.

Disease Outbreaks↗