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R Leirer

Publications and source records attributed to R Leirer.

13 recordsLinked to original sources
PubMed1993

Antigen capture ELISA using a monoclonal antibody for the detection of Mycoplasma bovis in milk.

An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (mAb) was developed to detect Mycoplasma (M.) bovis in milk samples from cattle. With this procedure, 1 x 10(5) colony forming units per ml (cfu/ml) milk were routinely detectable. No cross-reactions to other bovine mycoplasma species were observed. Both the sensitivity of 80.6% and the specificity of 94.9% are sufficient for its use in diagnosis of clinical mastitis. The sensitivity could be increased by 10% after introduction of 48-hour pre-incubation of samples. This allowed recognition of cows shedding M. bovis amounts of 10(3) cfu/ml in their milk, which is typical for subclinical cases. Screening of milk samples by means of this antigen capture ELISA has advantages over culture methods in terms of speed and potential to monitor large herds, thereby permitting early culling of infected animals to reduce transmission of the pathogen to non-infected animals.

Animals↗
PubMed1992

Preparation and characterization of monoclonal antibodies against Mycoplasma bovis.

Monoclonal antibodies (mabs) against Mycoplasma (M.) bovis were prepared for use in diagnosis of bovine mastitis. From the original 32 hybridomas actively secreting mabs against M. bovis, 6 stable lines were cloned. Two of them, Mb 5D8 and Mb 4F6, recognized M. bovis antigens of estimated molecular weights of 33 and 26 kDa, respectively. They showed no cross-reaction to other bovine mycoplasmas, thus rendering them useful for specific detection of this pathogen. All mabs investigated cross-reacted with M. agalactiae which is known to be closely related to M. bovis, but does not occur in cattle. Two other mabs, Mb 5D4 and Mb 1F6, exhibited further cross-reactions to a number of bovine mycoplasma species. Finally, mabs Mb 5D5 and Mb 2G5 reacted with all mycoplasmas tested. The possibility that they recognized constituents of the broth culture medium is discussed.

Animals↗
PubMed1989

[Experimental infections of bulls with Mycoplasma (M.) bovis and M. bovigenitalium].

M. bovis or M. bovigenitalium species experimentally used in intrapreputial infection were re-isolated from 7 of 8 bulls. Clinically manifest diseases did not develop at all, though slight inflammatory lesions were recorded from the genital tract of 2 animals. Mycoplasma findings are discussed together with results obtained from spermatological and hematological investigations.

Animals↗
PubMed1980

[Physiopathological studies of thyroid function in the calf. 1. Diagnosis of thyroid function in the calf].

Clinico-chemical and radiometric methods, common in routine diagnosis in human medicine for in-vitro assessment of thyroid function, were tested for their applicability to calf. The assessment of protein-fixed iodine and of iodine extractable from stock, the thyroxine test, the tri-iodothyronine test, the tri-iodothyronine radio-immuno assay, and the effective thyroxine ratio test, basically, were applicable to diagnosis of thyroid function in calf. The thyrotrophin releasing hormone test, in conjunction with efforts to determine peripheral thyroid gland hormone concentration (in particular tri-iodothyronine), was also applicable to calf, although thyroid stimulation hormone with radio-immuno assay, another procedure in human medicine (specificity of thyroid stimulation hormone antibody), proved unsuitable for the determination of bovine thyroid stimulation hormone.

Animals↗
PubMed1967

[Phagocytosis].

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Animal Diseases↗