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Biomedical subjects

R Lindner

Publications and source records attributed to R Lindner.

15 recordsLinked to original sources
PubMed1992

Clathrin-associated proteins of bovine brain coated vesicles. An analysis of their number and assembly-promoting activity.

In search of hitherto undiscovered coat proteins, clathrin-associated proteins (APs) from coated vesicles of bovine brain were affinity-purified by one cycle of coat assembly and fractionated by gel filtration on Superose 6. Immunochemical and gel electrophoretic analysis of the fractions revealed, besides AP180, auxilin, HA1, and HA2, a component with M(r) approximately 140,000. This protein (p140) is present in coated vesicles in about equimolar proportion to auxilin. The contribution of HA1, HA2, AP180, and auxilin to the total assembly activity in the Tris-soluble coat protein fraction were quantitatively analyzed by measuring the reduction in activity when each protein was removed from the mixture by immunoaffinity chromatography. It was found that AP180 accounts for 61% and HA2 for 33% of the activity, whereas auxilin, HA1, and p140 made a negligible contribution. Based on the relative molar concentration of APs in the coat protein fraction, AP180 is about 4 times more active in promoting clathrin assembly than are HA2 or the other APs.

Adaptor Proteins, Vesicular Transport↗
PubMed1992

[Integrated imaging of head and neck tumors in children].

Examinations of 28 children with various tumors of the visceral cranium and neck have shown that both ultrasound and magnetic resonance tomography should be used primarily for diagnosis and follow-up. These tomographic methods are especially suitable for use in children. Ultrasound is better suited to visualization of localized, superficial masses and MRT to examination of deeper lying tumors within the complex structures of the visceral cranium and neck. X-rays in different planes should be regarded as supplementary examinations. The use of CT, preferably as high-resolution CT, provides more precise visualization of fine bone structures. Conventional tomography has been almost completely replaced by CT. Angiography is applied mainly in subsequent, interventional measures; videofluoroscopy should be used to examine lesions of the upper airways and digestive tract.

Adolescent↗
PubMed1991

Light-chain-independent binding of adaptors, AP180, and auxilin to clathrin.

Binding of coated vesicle assembly proteins to clathrin causes it to assemble into regular coat structures. The assembly protein fraction of bovine brain coated vesicles comprises AP180, auxilin, and HA1 and HA2 adaptors. Clathrin heavy chains, separated from their light chains, polymerize with unimpaired efficiency when assembly proteins are added. The reassembled coats were purified by sucrose gradient centrifugation and examined for composition by SDS-PAGE and immunoblotting. We found that all four major coat proteins are incorporated in the presence and absence of light chains. Moreover, each of the purified coat proteins is able to associate directly with clathrin heavy chains in preassembled cages as efficiently as with intact clathrin. We conclude that light chains are not essential for the interaction of AP180, auxilin, and HA1 and HA2 with clathrin.

Adaptor Proteins, Vesicular Transport↗
PubMed1990

[Molybdenum as an air pollutant].

Investigations into the reasons for the retarded growth and discolouration of a small area of a field of rape situated on the outskirts of Vienna revealed higher than normal levels of molybdenum in the soil (up to 430 micrograms/l) and in the water (up to 9.7 mg/l). The source of the pollution was traced to a neighbouring industrial plant that was emitting the metal via the chimney stack. A review of the literature on the toxic effects of molybdenum in general and as an air pollutant in particular is provided. This shows that, in contrast to animals, this effect is relatively small in humans and plants. Nevertheless, the occupation-related inhalation of the metal has been shown to be associated with pneumoconiosis and gout-like symptoms.

Air Pollutants↗
PubMed1989

Acute subdural hematoma with and without multiple injuries.

A series of 109 patients with clotted subdural hematomas operated on within 72 hours of closed head injuries was evaluated retrospectively. Twenty-seven patients with multiple injuries showed a mortality rate of 77%, those without 59%.

Adolescent↗
PubMed1987

[Importance of MR tomography for the diagnosis of bone marrow diseases in childhood].

As a diagnostic and follow-up method of bone marrow diseases in children, magnetic resonance imaging (MRI) possesses special importance. It is characterised by high sensitivity in detecting bone marrow lesions, high soft tissue--bone tissue contrast, sharp demarcation of intraosseous lesions and visualisation of vascular structures without application of contrast medium. Thus it is able above all to take on the findings of radionuclide bone scanning, computed tomography and angiography. In the detection of pathophysiological alterations of the corticalis and of calcifications it is less reliable than x-ray images and CT.

Adolescent↗
PubMed1975

[Virus-like particles in yeast protoplasts. I. Electron microscopic demonstration].

In ultrathin sections and negatively stained isolates from protoplasts of a strain of Saccharomyces cerevisiae showing abnormal morphology and degeneration phenomena the presence of virus-like particles within the cells could be demonstrated. The isometric particles have diameters of about 50 nm, the electron dense centre is surrounded by distinct envelope. Protoplastic containing up to 30,000 particles could be found. It could be demonstrated that there exists a correlation between the amount of particles and the degree of degeneration of the cellular substructure. Protoplasts with high titres of virus-like particles obviously undergo lysis. Plaque formation on agar plates could not be seen. It is concluded that the phenomena observed can be compared with cytopathological effects caused by viruses in other cell systems.

Cytopathogenic Effect, Viral↗
PubMedDate not supplied

[Indications for proton spin tomography in central nervous system diseases in childhood. A 4-year experience report on 164 cases].

1. Magnetic Resonance Tomography (MRT) is the method of choice for precise, comprehensive and reproducible imaging of congenital malformations and space occupying lesions. 2. MRT indicates inflammatory processes of the CNS earlier than CT by which extension and localization are visualized more precisely. MRT gains particular importance for follow-up studies because there is no radiation burden. In cases of disseminated inflammatory lesions or concerning early diagnosis of Herpes-encephalitis an exact and rapid diagnosis is possible. 3. In patients suffering from convulsions with or without focal lesions in EEG MRT instead of CT should be applied as MRT allows three-dimensional imaging not influenced by bone artifacts. 4. CT is superior to MRT if small calcifications or acute hemorrhage are looked for. MRT enables us to differentiate several stages of bleeding. 5. In patients with cerebral AV-malformations angiography can be replaced by MRT. However, angiography is indispensable for small aneurysms and AV-malformations in the spinal canal. 6. Due to high soft tissue contrast MRT is the imaging modality of choice in patients with metabolic and degenerative diseases and disturbances of brain maturation. 7. In infants sonography should be done first and supplemented by MRT and/or CT.

Adolescent↗
PubMedDate not supplied

[New aspects in the diagnosis of intervertebral disk displacement of the upper lumbar spine with special reference to magnetic resonance tomography].

A report is presented on two patients who received surgery on a disc prolapse between the lumbar vertebrae 4 and 5 as treatment for compression of the fifth lumbar root, but in whom the clinically relevant disc prolapse was located higher (lumbar vertebrae 1/2 or thoracic vertebra 12/lumbar vertebra 1). Magnetic resonance tomography made a major contribution to the later diagnosis of the high disc prolapses. Besides precise localization of the lesion, MRT made it easier to understand why such a high disc prolapse can cause a compression syndrome of the fifth lumbar root.

Aged↗