Biomedical subjects

R M Binns

Publications and source records attributed to R M Binns.

At least 109 records · Page 6Linked to original sources

PubMed1977

Ferritin and ovalbumin binding blood lymphoid cells in fetal sheep.

Lymphoid cells from blood of fetal sheep between 70 and 140 days of gestational age were examined for surface immunoglobulin (sIg) and for surface binding of horse spleen ferritin FITC and ovalbumin FITC. Fetuses of all ages had cells which bound ferritin or ovalbumin, the proportion of these cells in the lymphocyte preparations from blood decreasing with increasing age of the fetus. A similar decline in the proportion of sIg cells with age was found. The simultaneous occurrence of fetal lymphoid cells binding ferritin or ovalbumin is discussed in relation to the sIg population and to the property of the sheep fetus to respond sequentially to different antigens by the production of antibody.

Animals↗

PubMed1977

Effect of thymectomy on lymphocyte subpopulations in the pig. Demonstration of a thymus-dependent 'null' cell.

Thymectomy in neonatal pigs results in marked lymphopenia. Surface Ig immunofluorescence, Fc rosettes and SRBC rosettes have been used to identify blood lymphocyte subpopulations. There was no marked reduction in numbers of cells in these subpopulations after thymectomy, although their proportion increased. This change was explained by a 90% reduction in 'null' lymphocytes, defined as those lacking sIg and Dextran SRBC receptor, which comprised 30-45% of blood lymphocytes.

Animals↗

PubMed1976

A mechanism of migration inhibition involving components of the coagulation system.

Lymph from pigs given bacillus Calmette-Guérin (BCG) and stimulated in the drainage area of a cannulated lymph node with tuberculin (PPD) had potent in vitro macrophage migration inhibitory activity. A similar observation has previously been made in sheep by other workers. In the sheep, part of, and in the pig, almost all of, this inhibitory activity was produced, not by a factor resembling the so-called 'migration inhibitory factor', but by a mechanism involving a protease and fibrinogen. It is suggested that exudate cells, having potent coagulation-promoting activity, can promote the deposition of fibrin about themselves, and thus 'trapped' are unable to migrate.

Animals↗

PubMed1975

Rosette formation with sheep erythrocytes--a possible T-cell marker in the pig.

A proportion of pig lymphocytes form rosettes with sheep erythrocytes. Factors affecting their demonstration have been investigated, and a standard technique defined. Rosette-forming lymphocytes lacked surface immunoglobulin detected by immunofluorescence and formation of rosettes was not inhibited by anti-immunoglobulin or anti-PLA sera, but was by anti-thymus serum. Of 18 species' erythrocytes tested only sheep, Barbary sheep and Mouflon erythrocytes formed rosettes in similar percentages. Fetal sheep erythrocytes formed no rosettes at 6o days of gestation and developed adult levels by term. Rosettes were formed by the majority of thymus cells, by only few bone marrow cells and by intermediate proportions of cells in other lymphoid tissues correlating with the probable order of T cell content. In pig fetuses, thymus contained postnatal levels of rosette-forming cells by 69 days, when such cells were not detected in other tissues. These data support the contention that SRBC rosettes are formed by T lymphocytes.

Animals↗

PubMed1975

Rosette formation of pig T lymphocytes with sheep erythrocytes.

The relationship of sheep RBC rosette formation to density of thymus and blood lymphocytes was investigated. Thymocyte density was unimodal and cells of all densities rosetted equally. Blood lymphocyte density was bimodal with most rosette-forming cells in the denser ficoll layers. Papain treatment of SRBC increases rosette formation with blood lymphocytes while apparently maintaining specificity of T cells.

Animals↗

PubMed1975

Immunoglobulin-bearing lymphocytes: their demonstration in adult sheep and ontogeny in the sheep fetus.

A method for the preparation of lymphocytes from sheep blood is described. Lymphocytes from adult and fetal sheep have been examined by immunofluorescence for surface immunoglobulin (sIg) determinants. Young adults (1.5 years) had a mean of 23.2% sIg cells, older adults (8.75 years) a mean of 9.7% sIg cells. Pregnancy did not influence these values. The earliest lymphocytes with sIg in fetal lambs were demonstrable at 52 days (96 mm crown-rump length) and countable by 56 days (110 mm CRL) at 0.3% sIg. The percentage rose rapidly to 15.1% between 78 and 87 days, followed by a fall to 2.1% around 117 days with a second increase thereafter. The significance of this spontaneous appearance of fetal sIg cells is discussed.

Aging↗

PubMed1974