PubMed Health⌕ Search

Biomedical subjects

R M Bradley

Publications and source records attributed to R M Bradley.

12 recordsLinked to original sources
PubMed1992

Functional regeneration of glossopharyngeal nerve through micromachined sieve electrode arrays.

To assess the potential of micromachined silicon sieve electrodes for long term recordings from single afferent sensory fibers, we implanted them between the cut ends of rat glossopharyngeal nerves which innervate taste and somatosensory receptors on the posterior tongue. After the implants had been in place for an average of 101 days nerve regeneration was measured using histological and electrophysiological methods. Axons of the glossopharyngeal nerve regenerated through holes in the sieves and supported the functional regeneration of taste, thermal and mechanoreceptors.

Animals↗
PubMed1978

Taste responses in sheep medulla: changes during development.

Response characteristics of taste neurons in the sheep solitary tract and nuclei alter during development. Solitary tract cells in younger fetuses respond to stimulation of the tongue with fewer salts and acids than do cells in older fetuses, lambs, and adults. Further, responses to specific salts and acids develop in a particular sequence, not randomly. These changes may relate to maturation of taste receptor sites.

Age Factors↗
PubMed1978

Effect of serum on ganglioside uptake and choleragen responsiveness of transformed mouse fibroblasts.

NCTC 2071 cells, transformed mouse fibroblasts, did not respond to choleragen when grown in chemically defined medium. When grown in medium containing 10 percent fetal calf serum, however, the cells accumulated cyclic AMP upon exposure to the toxin. Gangliosides isolated from the fetal calf serum were as effective as whole serum in inducing choleragen responsiveness in the cells. The putative choleragen receptor, the monosialo-ganglioside GM1, could not be detected by chemical analysis in cells exposed to serum. 3H-Labeled GM1 was detected in these cells, however, following sequential exposure to galactose oxidase and sodium borotritide. Thus, uptake of minute amounts of GM1 from serum by these cells sensitized them to choleragen.

Blood Physiological Phenomena↗
PubMed1978

Similar content of phospholipids and gangliosides in normal and homozygous familial hypercholesterolemia fibroblasts.

The cellular content of total and individual phospholipids and gangliosides was measured in fibroblasts cultured from four normal subjects, three patients with lysosomal lipid storage diseases, and two subjects with homozygous familial hypercholesterolemia. Measurements were made on cells grown in medium containing fetal calf serum under conditions in which normal cells derive cholesterol for cell growth from low density lipoprotein present in the fetal calf serum, whereas familial hypercholesterolemia homozygote cells, which lack cell surface low density lipoprotein receptors, derive cholesterol from endogenous synthesis. No difference was observed in the cellular content of total or individual phospholipids and gangliosides in the normal and familial hypercholesterolemia homozygote cells. In contrast, cells from a patient with Niemann-Pick disease and a patient with Sandhoff disease showed elevations in the content of sphingomyelin and complex gangliosides, respectively.

Adult↗