R M Colver | PubMed Health

PubMed1992

Laparoscopy: basic technique, instrumentation, and complications.

Laparoscopy has been an important diagnostic and therapeutic modality in gynecology for several decades. Recently, general surgeons and urologists have adopted laparoscopy to treat many disorders that formerly required an open laparotomy. Laparoscopic surgery requires additional instrumentation and skills as well as familiarity with a number of operative complications unique to laparoscopy. This overview discusses the basic equipment and instrumentation of laparoscopy and describes its possible associated complications.

Humans↗

PubMed1991

Influence of growth factors in defined culture medium on in vitro development of mouse embryos.

Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embryos cultured in Ham's F-10 alone versus growth factor (GF) supplemented media. Rates of development in the presence of GF were inhibited significantly in some cases, and the cell numbers per blastocyst were identical in all groups. When compared with in vivo derived blastocysts, however, all in vitro cultured embryos had significantly less cells per embryo. These results suggest that GF supplementation of media for culture of early preimplantation embryos does not stimulate the rate of embryonic development in vitro.

Animals↗