Monoclonal antibody enhancement in protein-free and serum-supplemented hybridoma culture.
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Amino Acids↗
Biomedical subjects
R M Fike
Publications and source records attributed to R M Fike.
Simplified cell microelectrophoretic method applied to the macrophage electrophoretic mobility test for cancer diagnosis.
Simplified agarose-coated capillary microelectrophoresis was adapted to the Macrophage Electrophoretic Mobility (MEM) test for cancer detection. Guinea pig alveolar macrophages gave superior reproducibility to peritoneal macrophages. As good or better reproducibility was obtained with cryopreserved, as with fresh macrophages. The electrophoretic mobilities of patients' lymphocytes themselves, after incubation with Encephalitogenic Factor (EF) showed a significant increase in electrophoretic mobility, while in control lymphocytes a decrease occurred. Thus, for the detection of the results of the interaction of EF on human lymphocytes, a direct lymphocyte electrophoretic mobility test may suffice, and guinea pig macrophages may no longer be required at all.
Animals↗
Zeta-protentials of intact cell monolayers determined by electro-osmosis.
By measuring the electro-osmotic flow velocity of buffer through a glass capillary, on the inner surface of which cells have been grown, the zota-protential of these cells can be determined without the changes in zota-potential, viability and morphology caused by the chemical, enzymatic or mechanical pretreatment hitherto necessary for cell dispersion in the microelectrophoretic method. Cells grown in a monolayer inside the capillaries were: BGM, HEP-2, and RPMI 1846 (the last one also could be grown in suspension).
Cell Line↗