Biomedical subjects

R M Guttman

Publications and source records attributed to R M Guttman.

4 recordsLinked to original sources

PubMed1985

Cryopreservation of skin using an insulated heat sink box stored at -70 degrees C.

The cooling of skin to a temperature of -70 degrees C was carried out by two methods: programmed controlled-rate (PCR) cooling at -1 degree C min-1 to -70 degrees C, and variable-rate cooling to -70 degrees C in an insulated heat sink box (IHSB). The IHSB was constructed of polystyrene and contained two aluminum heat sinks placed one on each side of flat packets of skin. The IHSB containing skin was cooled in a -70 degrees C constant-temperature refrigerator. When using the IHSB, the insulation provides a slow cooling rate while the paired heat sinks provide even heat flow across the top and bottom surfaces of the flat skin packets, minimizing the duration and potential damaging effects of the exothermic temperature plateau which occurs at the freezing point. When followed by 24-hr storage at -70 degrees C and warming at about 316 degrees C min-1, the IHSB cooling method was equivalent to the PCR method in generating a suitably slow cooling rate of -1 to -2 degrees C min-1, and maintaining about 80% of normal skin cell glucose metabolism. The development of the IHSB cooling system provides a method for the simple, cost-efficient cryopreservation of small amounts of autograft skin, such as those remaining from surgical procedures, and can also provide an allograft skin banking capability to any facility possessing a -70 degrees C refrigerator.

Animals↗

PubMed1979

Human lymphocyte-tumor cell interaction. A scanning electron microscopy study.

Cells from the metastatic chest wall lesions of a patient with adenocarcinoma of the breast were incubated with autologous lymphocytes. Their interactions were observed in the scanning electron microscope. The lymphocytes first surrounded the cancer cells; cancer cells then were destroyed while the lymphocytes in contact with them lost their microvilli. The heterologous human lymphocytes being used as controls surrounded the tumor cells but did not destroy them. Lymphocytes in the presence of tumor cells showed interconnections through cytoplasmic bridging.

Adenocarcinoma↗

PubMed1979

Morphologic expression of the interactions of human lymphocytes and Pseudomonas aeruginosa as observed by scanning electron microscopy.

When human lymphocytes and Pseudomonas aeruginosa were incubated together and then observed under the scanning electron microscope, four distinct morphologic observations were made: (1) filopods extending between lymphocytes and bacteria; (2) globular structures bulging out in many of the filopods; (3) filopods connecting lymphocytes to each other in the presence of bacteria; and (4) bacteria adhering to the lymphocytes in areas where decreased numbers of microvilli were present.

Adult↗

PubMed1975

Bacterial blocking activity of specific IgG in chronic Pseudomonas aeruginosa infection.

A factor has been demonstrated in the serum of some patients with chronic Gram-negative infections which specifically blocks bactericidal activity against the infecting organism. Sera with this factor, that had been obtained from patients suffering from chronic Pseudomonas aeruginosa infections, were fractionated by Sephadex G-200 and DEAE-Sephadex column chromatography. Blocking activity was detected in the Sephadex G-200 '7S' peak and eluted from the DEAE column along with the serum IgG. Immunoelectrophoresis studies of this material along with pure IgG showed that the bactericidal blocking factor in these patients was IgG. The blocking factor was specific in its ability to protect bacteria, and could be absorbed from the serum by the particular bacterial strain isolated from the patient. Possible clinical importance of blocking activity by IgG is suggested by the persistent nature of the Ps. aeruginosa infections in these patients.

Antibodies, Bacterial↗