Ultrastructure of potato yellow-dwarf virus.
Explore the source record for details and available documents.
Centrifugation↗
Biomedical subjects
R Markham
Publications and source records attributed to R Markham.
Stress, cognitive factors, and coping resources as predictors of relapse in alcoholics.
One hundred alcohol-dependent individuals attending a detoxification unit were assessed on a variety of psychological, social and demographic variables. Sixty-one participants were contacted at follow-up over 1 year later. Alcohol consumption was assessed through self-report and corroborative information. Self-reported levels of stress and social support were also obtained. High self-efficacy predicted low levels of self reported drinking at follow-up. Negative coping predicted higher levels of drinking as reported by the corroborator. High levels of stress in the month prior to follow-up were related to self-reported poor drinking outcomes, while ongoing social support since treatment was associated with favorable drinking outcomes. Overall, higher levels of self-efficacy during detoxification and social support following treatment were the best predictors of a favourable drinking outcome.
Adaptation, Psychological↗
An amplified ELISA for human tumour necrosis factor alpha.
An immunoassay for tumour necrosis factor alpha (TNF-alpha) is described using a sandwich system which employs a mouse monoclonal as the capture antibody and a polyclonal rabbit anti-TNF-alpha as the detection antibody. The assay utilises a novel enzyme cycling system to amplify the colourimetric signal generated by alkaline phosphatase in the enzyme immunoassay. The assay sensitivity is increased by the use of the AMPAK system. The detection limit of the assay is in the order of 1 pg/ml which is many times greater than is possible with conventional methods of measurement. The advantages of the enzyme-amplified method will be particularly useful in the quantitative determination of low levels of TNF-alpha in body fluids. This assay system was used to measure peripheral serum TNF levels in a group of women with non-malignant gynaecological conditions, the mean TNF-alpha concentration in these subjects was 10 pg/ml.
Animals↗