PubMed Health⌕ Search

Biomedical subjects

R Mauler

Publications and source records attributed to R Mauler.

36 records · Page 2Linked to original sources
PubMed1976

The use of new polymethylmethacrylate adjuvants for split influenza vaccines.

Split influenza virus vaccines with varying antigen content were adjuvated with polymethylmethacrylate particles, produced by polymerizing monomeric methylmethacrylate in the presence of the subunits, or by addition of the subunits to previously polymerized methacrylate particles. Both adjuvants yielded higher antibody titers than aluminum hydroxide or fluid vaccines. The character of the antigen-adjuvant conjugate of both types of polymer adjuvants is discussed.

Acrylic Resins↗
PubMed1976

Freeze-drying of a purified human diploid cell rabies vaccine.

A rabies vaccine prepared in human diploid cell strains was purified by continuous-flow sucrose density gradient centrifugation. The peak fractions containing 35-40% sucrose were diluted with a stabilizer consisting of degraded gelatine in a Tris-EDTA-buffer, and then inactivated with beta-propiolactone and freeze-dried to a residual moisture of approximately 2.5%. This vaccine was stable as proved by accelerated stability tests.

Cell Line↗
PubMed1975

Effect of neuraminidase on potency of inactivated influenza virus vaccines in mice.

The protective effect of neuraminidase was studied in a mouse protection test using isolated neuraminidase of A2/Aichi/68(H3N2) virus and the complete recombinant virus A/eq(Heq-1)-HK(N2) as antigens. Immunized mice were protected against A2/Aichi(H3N2) challenge virus; however, the protection rate was low in comparison to animals immunized with comparable amounts of the complete A2/68(H3N2) virus9 Furthermore there was no cross-protection against A2/Asia/57(H2N2) challenge virus. The protective effect of neuraminidase was not impaired by Tween-ether treatment of the A/eq(Heq-1)-HK(N2) recombinant virus.

Animals↗
PubMedDate not supplied

Results of the single-radial-diffusion test with formaldehyde-treated influenza virus.

We found reduced values for formaldehyde-treated influenza virus in the single-radial-diffusion test by comparison with non-treated virus. The reduction correlates with the formaldehyde concentration. Hemagglutination and neuraminidase activities were not affected by the same treatment. We therefore suggest that this reduction is not based on antigen destruction.

Formaldehyde↗
PubMedDate not supplied

Reactogenicity to primary and repeated vaccination with influenza split virus vaccine.

Influenza vaccines (split, adsorbed, low nitrogen) of two different antigen concentrations (commercial production) were administered to 1,111 adult persons of both sexes. Up to five previous vaccinations had been given to the population under observation. Their reactions both local and general were not different in primo-vaccinees or boostered persons. Increasing numbers of previous vaccinations were not reflected by increasing rates of complaints. Disregarding immunization history, the group given the higher concentrated vaccine showed a lower rate of side effects while the lower antigen concentration produced a higher rate. The relevance of the observation that no sensitizing effect occurred in persons with up to five vaccinations may be a characteristic of split adsorbed influenza vaccines of high purity.

Adult↗
PubMedDate not supplied

Interference of preceding BCG vaccination with BCG immunotherapy in L2C-guinea pig leukemia.

Guinea pigs of inbred strain 2 were vaccinated with BCG 1331 Copenhagen (K-Nr. 240-1) six to seven weeks before intradermal application of about 10(5) leukocytes from a highly leukemic animal in mixture with 10(7) colony forming units (CFU) of BCG. This presensitization with BCG totally abolished or strongly reduced the antitumor activity of L2C-cells adjoining BCG. Increase of bacterial mass applied from 1 X 10(7) to 2 X 10(8) CFU did not restore the antileukemic effect of BCG as observed in unvaccinated guinea pigs by a significant delay of death in a portion of animals and by the occurrence of long-term survivors. Since local reaction to BCG occurred much earlier in presensitized than in unvaccinated animals, we speculate that the destruction of tumor cells and hence the antigenic stimulus was too premature in presensitized animals to build up a BCG-mediated antitumor immunity.

Animals↗
PubMedDate not supplied

Zonal-centrifuged human diploid cell rabies vaccine.

In order to reduce the risk of allergic reactions, a purified human diploid cell culture rabies vaccine was developed. Pitman-Moore strain of rabies was propagated in WI-38 or MRC-5 cells. Both cell strains were found to be equally suitable for pro agation of rabies virus. The virus was purified and concentrated by continuous-flow sucrose density gradient ultracentrifugation. Virus peak fractions were diluted with a stabilized and then inactivated with BPL and freeze-dried. According to the standard NIH test for potency, the vaccine proved to be very potent and stable. The results of initial clinical applications of the vaccine will be presented.

Antibody Formation↗