[Tubular inclusions within microbodies of Saccharomycopsis (Candida) lipolytica-protoplasts (author's transl)].
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Biomedical subjects
Publications and source records attributed to R May.
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Cells of Saccharomyces carlsbergensis contain proteins assembling to crystals and bundles of tubules (tb) in the cyto- and karyoplasm by osmotic shock with hypertonic solutions. The cellular concentration of these proteins is regulated by oxygen pressure during growth. In cells grown at optimal aeration the protein level is low and crystals and tb cannot be induced. After a short period of O2-limitation or anerobic growth conditions the protein concentration increases and induction of crystals and tb is possible.
When grown in a medium containing lactat, Saccharomyces carlsbergensis produces 5 times more ground-plasmatic ADH than Kluyveromyces fragilis. Upon gelectrophoresis, K. fragilis exhibits 7 bands while S. carlsbergensis shows only one. In a polyacrylamid gradient, the ADH's of both strains yield one band, the position of which corresponds to a molecular weight of about 160 000 D. In cell homogenates and mitochondrial fractions of S. carlsbergensis treated with ultrasound, an ADH is detected which exhibits 3 subbands and a molecular weight greater than 1 Megadalton. This ADH does not occur in K. fragilis. Mitochondrial fractions from K. fragilis contain an ADH, the electrophoretical mobility of which is identical to that of the ADH of the groundplasma. Nuclei of S. carlsbergensis also possess ADH whereas those of K. fragilis probably do not.
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The most important preliminary examinations are: the visualisation of the valves of the veins by retrograde strain phlebography, determination of the degree of the recanalisation of the deep veins and the outflow of the pump of the calf muscle. Venous edemas: congenital absence of the valves of the veins, intramural thrombosis, postthrombotic edemas, division into four degrees, combined venous-lymphatic edemas.
In the E. coli 50 S ribosomal subunit, proteins L7/L12 and L10 were deuterated by partial reconstitution. The distance between L7/L12 and L10 was measured by the label triangulation method and was found to be approximately 100 A or, with low probability, 60 to 70 A, depending on the concentration.
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1. Local rupture of the veins by fracture. Compression and local spiral thrombi. 2. Failure of the venous pump at the ankle joint due to immobilization. 3. Failure of the pump in the calf muscles, leading to propagation of thrombi in the venae gastrocnemiae and in the veins of the soleus muscle, frequently with varicosity. 4. In 22 percent of left-sided fractures a venous spur is found in the left V. iliaca com.; increased likelihood of thrombosis in left leg in case of stasis due to immobilization.
Defining "symbol" as that which draws together and unites experience and "myth" as a cluster of symbols set in dramatic form, the author discusses the lack of both symbols and myths in contemporary sociaty. This lack had led to a disintegration of commonly held values and, ultimately, to a great need for psychotherapy on the part of individuals seeking personal identity. The individual must define his or her own values according to personal myths. One basic function of psychotherapy is to help individuals in their attempt to recover values.
Four studies were done to explore the construct validity of a previously established sex difference in TAT fantasy patterns. The results, both with various clinical groups and with normal children, support the notion that Deprivation/Enhancement fantasy patterns are meaningfully related to sexual identity and sex role development. The correlates of extreme D/E patterns are more clear for women than for men and involve elements of the "hysterical" character and cognitive style.
A purified fraction of crystals stabilized with Cd2+ is prepared from Saccharomyces carlsbergensis protoplasts by means of differential centrifugation. As proved by negative staining the crystals are hexagonal with well preserved surface which in its turn also reveals a hexagonal fine structure. Crystals are digestible by pronase (1 mg/m) in phosphate buffer (pH 7.4). The molecular weight of protein subunits is 38000+/-300D. Amino acid composition of the crude crystal protein is similar to that of microtubular protein (MTP) from brain and sperm, except that lysin content is much higher. The crystal protein exhibits more differences, however, after purification by preparative gel electrophoresis. The level of glycine, alamine, and valine is increased, whereas that of glutamic acid is decreased.
This paper concerns a common clinical picture, one that is seen particularly clearly in young men. It is a picture which, within a traditional psychoanalytic framework, has much to do with that set of mental structures referred to as the superego and with the patterns which follow from the oedipal period of development. Using two case examples, this paper suggests the outlines of a treatment strategy and looks at some of the theoretical issues involved (in particular, the contribution of an object relations emphasis to classical theory). It argues for the importance of grief in the oedipal dilemma and suggests that the role of grief in the formation and maintenance of morbid conscience can overshadow that more familiar explanation, the struggle with one's own forbidden wishes.