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Biomedical subjects

R McKinney

Publications and source records attributed to R McKinney.

3 recordsLinked to original sources

Emotional complications of adolescent grand mal epilepsy.

Adolescents who have grand mal epilepsy and their parents were interviewed, and the adolescents were evaluated neurologically. Better seizure control and less neurologic disability were unexpectedly associated with less open communication between the adolescents and their families and friends, and with a poorer self-image and poorer expectations for the future. These findings were unrelated to IQ or school performance. This outcome is consistent with other studies of invisible defects and stigmatization, and suggests that youngsters who have relatively mild defects involving social disability may be more troubled than those with more apparent defects, for which denial may be operative. An incidental finding in the study was that a question more predictive of overall family, social, and academic function than the neurologic findings was simply whether or not the youngster was attending the appropriate grade in school for his or her age.

Adolescent

Conjugation methods in immunofluorescence.

We have described methods of labeling antibody preparations with FITC, TMRI, and RBI. The degree of labeling with FITC can be precisely controlled by using well-defined conjugation procedures and FITC of a known degree of purity. Our experience shows that relatively high F/P ratios of the order of 20 to 25 mug/mg are desirable for antibacterial conjugates. Many commercial preparations of rhodamine isothiocyanate are of very poor quality and are unsatisfactory for use in conjugate preparation. Therefore, one should analyze the rhodamine isothiocyanate product before preparing immune conjugates. Our experience indicates that very satisfactory conjugates of immune IgG or pure antibody can be prepared with TMRI of about 60% purity by using a dye-protein ratio of 20 mug/mg. The optimal dye-IgG ratio for labeling with RBI appears to be about two times that for labeling with TMRI because of the lower specific absorbance and fluorescence emission of RBI. Rhodamine conjugates may be preferred to FITC conjugates in certain situations where tissue autofluorescence interferes with the observation of the yellow-green emission of FITC. Furthermore, mixed rhodamine and FITC conjugates of different specificity can be used to great advantage in double-staining techniques that allow simultaneous screening for two antigenically different organisms on a single microscope slide.

Fluoresceins

Effect of metabolic inhibitors on bone lipid synthesis from radioactive bases.

Iodoacetate at 10(-3) M and 10(-4) M concentrations was found to strongly inhibit incorporation of both 14C-choline and 3H-inositol into phospholipids of calvaria from newborn rats; it had somewhat less effect on ethanolamine incorporation and almost no effect on serine incorporation. Similar though less dramatic results were observed with cyanide or dinitrophenol at similar concentrations.

Animals