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R McMillin

Publications and source records attributed to R McMillin.

5 recordsLinked to original sources

Complications of acquired diverticula of the ileum.

Acquired diverticula of the small bowel are extremely rare. These diverticula occur in less than one per cent of the population. Acquired diverticula of the ileum make up approximately ten per cent to 20 per cent of this number. Associated complications are noted in less than ten per cent of patients, but mortality rates related to these complications have been reported to range from 25 per cent to 50 per cent. Major reported complications include perforation, hemorrhage, and obstruction secondary to inflammation. Our experience with three cases of complicated ileal diverticula is presented. All patients presented with physical findings consistent with an acute abdomen, but the correct diagnosis was not made in any of these patients preoperatively. Two patients were found to have perforation of an ileal diverticulum: one with localized abscess formation and the other with diffuse peritonitis. The third patient had ileal diverticulitis without perforation. Two of the patients had resection and primary anastomosis. The third patient had resection and an ileostomy and mucous fistula created. Postoperatively, one patient died of a massive cerebrovascular accident. A thorough discussion of the literature concerning these rare acquired ileal diverticula is presented. Clinical manifestations, complications, and associated surgical therapy are discussed. Usually, resection of the involved segment with primary anastomosis can be performed.

Aged↗

Ischemic myocardial injury in cultured heart cells: leakage of cytoplasmic enzymes from injured cells.

An in vitro model of myocardial ischemia has been established with primary monolayer cultures of postnatal rat myocardial cells. Ischemic conditions were simulated in vitro by subjecting the myocardial cell cultures to various levels of oxygen and glucose deprivation. The experimental protocol consisted of treatment with 20% or 0% O2 and 1000, 500 or 0 mg glucose per 1 of medium for 4 or 24 hr. Control cultures were treated with 20% O2 and 1000 mg glucose. After the ischemic treatments, cultures of beating muscle (M) cells were evaluated for signs of injury, i.e. leakage of cytoplasmic enzymes into the culture medium. Differences were found in leakage of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) from the cultures that were exposed to partial ischemia of glucose deprivation and from those cultures that were exposed to total ischemia of oxygen and glucose deprivation. Glucose deprivation along resulted in a slight-to-moderate loss of LDH and CPK from the cells, whereas total ischemia resulted in a significant release of the two cytoplasmic enzymes. When the cultures were allowed to recover after ischemic treatment in complete medium (1000 mg glucose) and a normal atmosphere of 20% O2, they had levels of LDH leakage comparable to those of control cultures. Cell viability and total protein content of the ischemic cultures did not differ significantly from controls.

Cell Survival↗