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Biomedical subjects

R Mendel

Publications and source records attributed to R Mendel.

5 recordsLinked to original sources
PubMed1987

[Highly differentiated adenocarcinoma in a villous adenoma of the common bile duct near the papilla].

A 65-year-old man, in hospital for treatment for bladder carcinoma, was by chance found also to have a so far asymptomatic obstructive jaundice with hydrops of the gallbladder. Endoscopic retrograde cholangiography revealed, in addition to a papilla distorted by tumour, a clearly obstructed choledochal duct with dilated intrahepatic bile ducts, caused by a 2-3 cm prepapillary intraductal choledochus tumour, which in the biopsy specimen corresponded to papillomatous structures with occasional medium-grade epithelial dysplasias. The patients, who initially declined operation, was five weeks later re-admitted with the clinical picture of an acute abdomen. In view of the history the surgeon performed a partial duodenopancreatectomy despite the acute emergency. Histology revealed a highly differentiated adenocarcinoma within a villous adenoma of the choledochal duct near the papilla.

Adenocarcinoma↗
PubMed1986

A binary vector for transferring genomic libraries to plants.

The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes. The small genome of Arabidopsis thaliana is a good candidate for attempting this so-called shotgun transformation. We present the properties of an A. thaliana genomic library cloned in a binary vector, pC22. This vector, designed to introduce genomic libraries into plants, contains the oriV of the Ri plasmid pRiHR1 by which it replicates perfectly stably in Agrobacterium. Upon transfer of the library from E. coli to A. tumefaciens large differences in transfer efficiencies of individual recombinant clones were observed. There is a direct relation between transfer efficiency and stability of the recombinant clones both in E. coli and A. tumefaciens. The stability is independent of the insert size, but seems to be related to the nature of the insert DNA. The feasibility of shotgun transformation and problems of statistical sampling are discussed.

Cloning, Molecular↗