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R Mestel

Publications and source records attributed to R Mestel.

3 recordsLinked to original sources
PubMed1997

[The ideal image and sex role norms in the Giessen Test].

This study was intended to test the assumption whether the ideal-image measure within the Giessen-Test reflects the specific personality as hypothesized by psychoanalytic theory or a general norm-orientation. To test this assumptions the items of the Giessen-Test were transformed an submitted to random samples of in- and out-patients. The results clearly indicate that the ideal-image as measured by the Giessen-Test is a general norm-orientation and consists of the dimensions "social-acknowledgement" and "capability of binding". Within the second dimension there seems to be a gender difference. Additional analysis of variance and Cronbachs alpha indicate that differences between in- and out-patients are the result of a possible "curative effect".

Adult↗
PubMed1989

Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities.

Sequences within the spacer region of yeast rRNA cistrons stimulate synthesis of the major 35S rRNA precursor in vivo 10- to 30-fold (E. A. Elion and J. R. Warner, Cell 39:663-673, 1984). Spacer sequences that mediate this stimulatory activity are located approximately 2.2 kilobases upstream from sequences that encode the 5' terminus of the 35S rRNA precursor. By utilizing a centromere-containing plasmid carrying a 35S rRNA minigene, a 160-base-pair region of spacer rDNA was identified by deletion mapping that is required for efficient stimulation of 35S rRNA synthesis in vivo. A 22-base-pair sequence, previously shown to support RNA polymerase I-dependent selective initiation of transcription in vitro, was located 15 base pairs upstream from the 3' boundary of the stimulatory region. A 77-base pair region of spacer DNA that mediates transcriptional terminator activity in vivo was identified immediately downstream from the 5' boundary of the stimulatory region. Deletion mutations extending downstream from the 5' boundary of the 160-base-pair stimulatory region simultaneously interfere with terminator activity and stimulation of 35S rRNA synthesis from the minigene. The terminator region supported termination of transcripts initiated by RNA polymerase I in vivo. The organization of sequences that support terminator and promoter activities within the 160-base-pair stimulatory region is similar to the organization of rDNA gene promoters in higher organisms. Possible mechanisms for spacer-sequence-dependent stimulation of yeast 35S rRNA synthesis in vivo are discussed.

Base Sequence↗