Biomedical subjects
Publications and source records attributed to R Milbradt.
In an 80-year-old woman with pemphigus erythematosus, we demonstrated ANA as well as anti-DNA antibodies in the serum. This finding supports the argument that this skin disorder represents a combination of a disease of the pemphigus group with systemic lupus erythematosus.
Anthralin is an inhibitor of oxidative phosphorylation at concentrations found in vivo. ADP-stimulated oxygen consumption is diminished. Consequently, the rate of ATP synthesis is reduced and mitochondrial ATP content declines. Neither the isolated ATPase (F1F0-ATPase), nor the mitochondrial membrane-bound ATPase are influenced by the drug. Respiration under resting conditions is not affected. The experimental data unequivocally indicate that anthralin is not an uncoupler of oxidative phosphorylation, as previously stated. Furthermore, the interpretation that respiratory deficiency induced in yeast strains by anthralin is a consequence of petite mutations has to be reconsidered. Under in vivo conditions, anthralin inhibits respiration per se. Our experiments, including the electron spin resonance spectroscopy, reveal that anthralin alters mitochondrial membrane structure and function simultaneously. A redox or free-radical mediated step may be involved. In consequence, inhibition of ATP production occurs which may become the limiting factor for increased cellular metabolism in psoriasis.
Explore the source record for details and available documents.
Temocillin has pronounced in vitro activity against both penicillin-sensitive and penicillinase-producing gonococci. A single intramuscular injection of temocillin 0.5 g or 1.0 g resulted in the disappearance of the infecting gonococci in 91% of 75 patients and 94% of 192 patients in 2 separate trials. The systemic and local tolerance of the drug was unusually favourable and no serious side effects were observed.
This report describes the successful treatment of facial seborrheic dermatitis with a low-dose dithranol preparation. The results of a pilot study involving 18 outpatients are presented.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The first electron-microscopic description of DTIC-induced human liver injury is presented. A 61-year-old man developed signs of hepatic failure during the second treatment cycle with DTIC for malignant melanoma. Light-microscopic examination revealed extensive centrilobular liver necrosis. Terminal hepatic venules did not show any signs of vasculitis or thrombosis and there was a lack of inflammatory infiltration. At the ultrastructural level intracytoplasmic, membrane-bound, organelle-free vacuoles were found in the hepatocytes. Liver cells showed bleb formation. Bile canaliculi were dilated and their microvilli flattened. In the pericanalicular exoplasm electron-dense fibrillary material, thought to be of microfilamentous origin, accumulated. The patient received 250 mg methylprednisolone i.v. at the very onset of symptoms and was discharged 12 days after the peak rise of transaminases with normal liver parameters.
By means of a teleangiectasia macularis eruptiva perstans (Weber), observed in a 65 year old female patient, the coordination to urticaria pigmentosa is discussed as well as the differential diagnosis.
Explore the source record for details and available documents.
Combination of animal specific antigen reactions and tissue specific factors were found in a female patient with protein dermatitis (Jorth). Positive reactions to muscle-tissue of pigs but no reaction to renal tissue of pigs, tested intracutaneously were obtained. These observations suggest, that muscle specific aminoacids 3-methyl-histidine, monomethyl lysin and tri-methyl-lysin cause antigen reactions.
The effect of a commercial deproteinized blood extract--on motility of human spermatozoa was examined in vitro. The improvement of motility as seen after addition of 5 and 10 percent of this deproteinized blood extract, is supposed to be due to amounts containing different concentrations of LH. The extract was ruled out as a source of kallikrein, but not as a source of kininogens.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
100 women, routinely and regularly examined in a venereal disease clinic, have been investigated using Papanicolaou smears of the uterine cervix for. 1. Papanicolaou group, 2. trichomonas infection, 3. assessment of vaginal microbiology. A group III was determined in 11, a group IV/V in 2 women. Trichomonas vaginalis was present in 60% of the cases. Only 9% of the smears showed normal Döderlein bacteria; 91% contained mixed bacteria. The findings are compared with a control group and are discussed in the light of epidemiologic studies indicating the close rôle of certain factors in the sexual life for the development of cervical cancer. The necessity to regard this population as a high risk group in general cytological screening has been stressed, especially to the advantage of the adolescent and young females in this group.
6 cases of acrodermatitis papulosa eruptiva infantum (acrodermatitis papulosa infantilis or Gianotti-Crostisyndrome: G.C.S.) were observed and the clinical features of this disease are discussed. 1. An erythemato-papular dermatitis mainly of the face, legs, arms, buttocks, not itching, without re-occurrence lasting on the average 20--25 days. 2. An distinct enlargement of the lymph nodes, especially of the inguinal and axillary areas (reactive reticulo-histocytic lymphadenitis). 3. Acute hepatitis, mostly without icterus. 4. Presence of HB Ag-Australia Antigen in the serum of patients a few days after the onset of the disease. Doubts concerning the entity of G.C.S. are getting irrelevant considering the distinct characteristics. The viral genesis already suggested by Gianotti and Crosti of the picture of the disease seems to be true: in all cases Australian antigens are positive. The infectiousness, but the small danger of infection, even if the disease dates back a few months, should not be underestimated. When diagnosing G.C.S. among others the akrolocalized papular-vesicular syndrome described by Crosti and Gianotti should be considered. In 2 patients we could find by electron microscopic studies microtubular aggregates having a diameter of 200 A. These aggregates were situated in the cytoplasm of endothelial cells of smaller vessels in the upper part of the corium.