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Biomedical subjects

R Morelli

Publications and source records attributed to R Morelli.

At least 91 records · Page 5Linked to original sources
PubMed1981

Generation of suppressor cells in the response of human lymphocytes to a polysaccharide from Candida albicans.

MPPS stimulation of human lymphocytes is characterized by a 2-way cooperation phenomenon, i.e., T help of B cells and B help of T cells. In this system suppressor cells are generated after 6 days of culture. They were characterized as Ia- T cells. Their suppressor activity affected both T and B cell stimulation, and it may involve 2 different mechanisms: direct cell suppression, and interaction with soluble helper factors.

Animals↗
PubMed1981

Mitogenic response of human peripheral blood lymphocytes to a purified C. albicans polysaccharide fraction: lack of helper activities is responsible for the in vitro unresponsiveness to a second antigenic challenge.

Human peripheral blood lymphocytes primed with MPPS (a polysaccharide fraction extracted from C. albicans) were unable to respond to a second challenge of the same antigen. The proliferative response of these lymphocytes was restored by both mitomycin C-treated fresh autologous lymphocytes and by helper factors. Removal of Ia+ cells from fresh lymphocytes abolished the helper effect. On the other hand, when primed lymphocytes were depleted of Ia+ cells, the helper factor-responding cells were eliminated, whereas Ia- cells maintained the capacity to respond to fresh autologous lymphocytes. A model for cell-cell cooperation involved in the regulation of the immune response to this antigen is proposed.

Antigens↗
PubMed1980

Human lymphocyte-activating properties of a purified polysaccharide from Candida albicans: B and T cell cooperation in the mitogenic response.

Purified human T and B lymphocytes were tested for their ability to show a mitogenic response to a purified polysaccharide (MPPS) extracted from C. albicans. It was found that neither T nor B cells alone could respond to MPPS. When a mixture of these two types of cells was tested, a normal response (comparable to the one of unfractionated peripheral blood lymphocytes) was observed. This response was not affected by prior depletion of macrophages. Results obtained with normal B or T cells mixed with mitomycin C-treated T or B cells indicated that a reciprocal helper effect exists between these two types of cells in their response to MPPS. This response could be inhibited by antisera against HL-A and Ia-like antigens.

Animals↗
PubMed1979

[The physician-patient relationship in the acupuncture treatment of psychosomatic headaches].

Authors take in exame the acupuncture treatment in a group of subjects suffering from periodical cephalalgia about psychosomatic nature. Particular attention comes put considering the importance of relation MD-patient that one fixes in acupuncture, such as to consent a better elaboration about somatic symptom. MD always must be attentive in valutation of meaning about presented disturb and eventual latent message.

Acupuncture Therapy↗
PubMed1975

[Technic and indications for acupuncture in psychiatry].

On the basis of personal experience in the People's Republic of China, Chinese Psychiatry and acupuncture are reviewed. The application technique is described and the syndromes in which it is most indicated discussed. Its association with psychodrugs is also considered.

Acupuncture Therapy↗
PubMed1971

Role of complement during experimental Candida infection in mice.

The role of complement during experimental Candida albicans infection was investigated in mice. It was found that complement-positive CF-1 mice, infected with the test organism, survived significantly longer than complement-deficient mice of the same strain. This implies the participation of the relatively late acting complement component in host defense mechanisms directed against this systemic mycotic infection.

Journal Article↗
PubMedDate not supplied

Cryoglobulinaemia in a patient with Proteus mirabilis sepsis.

Mixed polyclonal cryoglobulinaemia was evidenced in a 49-year-old woman admitted to our hospital because of Proteus mirabilis sepsis associated with polyarthralgia and purpuric manifestations on the lower limbs. Cryoglobulins and circulating immune complexes decreased during the second week of illness and disappeared after recovery. CH50, C3 and properdin factor B, which were low during the early phase of the illness, returned to normal; C4 was normal throughout. The rapid clearance of cryoglobulins and immune complexes and the restoration of a normal complement profile might all be explained by the gradual elimination of P. mirabilis due to chemotherapeutic treatment.

Complement C3↗
PubMedDate not supplied

Isolation of a human serum protein that inhibits the growth of Cryptococcus neoformans.

Human serum at 5 to 10% (v/v) in tissue culture medium RPMI-1640, inhibits the growth of Cryptococcus neoformans by 80 to 93%. Serum fractionated on molecular sieve columns (Sephadex G-200) yielded an active protein fraction. This fraction at 100 micrograms protein/ml inhibited the growth of C. neoformans by 54%. When an active G-200 fraction was applied to a dye affinity column (Affi-Gel Blue) the fraction with inhibitory activity was bound by the column and was eluted with 1.4 M NaCl in 0.1 M phosphate buffer (pH 7.4). The bound fraction at 62.5 micrograms protein/ml inhibited C. neoformans growth by 82%. On native polyacrylamide gel electrophoresis (Nu-PAGE) the bound fraction migrated as a major and a minor band. Under the reducing conditions of sodium dodecyl sulfate (SDS)-PAGE the bound fraction yielded 4 prominent bands with MW ranging from 175 kDa to 45 kDa. Purification of the active Sephadex G-200 peak was achieved using an anion exchange column (DEAE-Sephacel). Protein eluted with 0.1 M NaCl had strong anticryptococcal activity (12.5 micrograms/ml, 79% inhibition), which in SDS-PAGE migrated as a single band with an approximate MW of 85 kDA. This protein appears important in natural host resistance to C. neoformans and polymorphisms or deficiencies may have epidemiologic and diagnostic relevance.

Antifungal Agents↗