PubMed Health⌕ Search

Biomedical subjects

R Motta

Publications and source records attributed to R Motta.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

Effects of a new loop diuretic (muzolimine) in cirrhosis with ascites: comparison with furosemide.

Muzolimine is a loop diuretic with both the dose-dependent increasing effectiveness of loop diuretics and the long-lasting effect of thiazides. This is a potential advantage in the treatment of ascites in advanced cirrhosis since these patients have a low tolerance to sudden reductions of blood volume. Equivalent single, oral doses of furosemide (40 mg) and muzolimine (30 mg) were given to 10 cirrhotic patients with ascites and reduced renal perfusion (glomerular filtration rate = 30 to 75 ml per min). The study was preceded by 4 days of equilibration (dietary sodium 40 mmoles per day), and the drugs were alternated via a single-blind, cross-over protocol after a wash-out period of 3 days. Renal function was monitored under basal conditions and after diuretic administration through 4-hr clearance periods for 24 hr. The renin-aldosterone axis was evaluated before diuretic administration and after 8 and 24 hr. Muzolimine led to a 12-hr cumulative diuresis [AUC0-12 = 2.52 +/- 0.42 (S.E.) ml per min] and natriuresis (5.14 +/- 1.05 mmoles per hr), which were comparable to those of furosemide (2.85 +/- 0.29 ml per min and 6.75 +/- 1.63 mmoles per hr). Its effect, however, was distributed over a longer period (8 hr) than furosemide (4 hr). Muzolimine activity mainly differed from furosemide because of: significantly lower 12-hr potassium excretion (AUC0-12 = 0.28 +/- 0.82 vs. 2.69 +/- 0.46 mmoles per hr; p less than 0.005); greater sodium/chloride excretion ratio (0.45 +/- 0.08 vs. 0.26 +/- 0.06; p less than 0.025), and absence of rebound phenomena.(ABSTRACT TRUNCATED AT 250 WORDS)

Aldosterone↗
PubMedDate not supplied

Comparative analysis of [3H]-thymidine labelling index and monoclonal antibody Ki-67 in non-Hodgkin's lymphomas.

The relation between the [3H]-thymidine labelling index (3H-Thy LI), which evaluates the S-phase cells, and the monoclonal antibody Ki-67 (MoAb Ki-67), which recognizes a nuclear antigen expressed during the cell cycle, was defined in 35 non-Hodgkin's lymphomas (NHL). Significantly higher median values of [3H]-Thy LI and Ki-67-positive cells were observed for high-grade than for low-grade malignancy tumours according to the Kiel classification, but with wide and overlapping values for the two morphologic subgroups. A significant correlation was observed between [3H]-Thy LI and Ki-67-positive cells (P less than 0.0001; r = 0.62). However, the ratio between the two cell kinetic variables on individual tumours was not constant. It sharply increased with decreasing [3H]-Thy LI values and was much higher in low-grade NHL than in high-grade NHL. Follow-up studies are needed to establish the role of the tumour growth fraction as evaluated by MoAb Ki-67 as a prognostic indicator in NHL.

Adolescent↗
PubMedDate not supplied

Strong allelic selection in the course of brother x sister matings in mice.

A set of 36 recombinant congenic mouse strains, resulting from crosses between B10.D2 and DBA/2, was created. During the backcross phase, animals were selected for their sensitivity to the graft-versus-host reaction induced by a B10.D2 hematopoietic cell transplant after irradiation. The progress of inbreeding was followed throughout the brother x sister phase by determining the allelic form of 14 genetic markers. During the backcross phase, the mean selective value per generation and per marker for the heterozygous allelic combination was 2.81 (1 for the homozygous B10.D2). During the brother x sister phase, this value was 1.15 and 0.30 for the heterozygous and the DBA/2 homozygous combinations, respectively (1 for the homozygous B10.D2). The origin of this strong allelic disadvantage for the DBA/2 homozygote, in the face of countervailing selection, is discussed. These data strongly suggest coadaptation between alleles constituting the genome of the inbred strains of the laboratory mouse, resulting in strong counterselection of the alleles derived from one inbred strain when a small amount is spread over the genetic background of another inbred strain.

Alleles↗
PubMedDate not supplied

Analysis of B10.D2 recombinant congenic mouse strains shows that audiogenic and beta-CCM-induced seizures depend on different genetic mechanisms.

The rate of occurrence of audiogenic seizures and seizures induced by the benzodiazepine receptor inverse agonist methyl-beta-carboline-3-carboxylate (beta-CCM) were analyzed in several recombinant congenic strains of mice bred from B10.D2 and DBA/2J. Although both types of seizures have similar behavioral patterns and might involve GABAergic mechanisms, no correlation was observed between the occurrence of the two types of seizures across the strains, suggesting that these two types of seizures depend on different genetic mechanisms.

Acoustic Stimulation↗
PubMedDate not supplied

[Incidence of restrictive measures in biological research].

Regulations on the use of laboratory animals are being in process, with regard to the very different aspects of animal experimentation. The generalization of feelings of respect for animals is highly positive. Research workers should apply themselves to avoiding this progress in human behavior to be associated with the development of "anti-science" reactions, leading to guilty feelings about the mainspring of their activity.

Animals↗