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Biomedical subjects

R Musoke

Publications and source records attributed to R Musoke.

7 recordsLinked to original sources
PubMed2006

nef gene sequence variation among HIV-1-infected African children.

BACKGROUND: There are few data on African children infected with nonclade B HIV-1 in endemic settings, which limits generalizations about pathogenesis and progression. Genotypic and phenotypic variations in host immunogenetics and HIV-1 negative factor (nef) accessory protein may influence disease progression and have frequently been characterized in subjects infected with clade B HIV-1. METHODS: In this descriptive study, we report nef gene sequence variation and host genetic polymorphisms in 32 Kenyan children, including 12 slow progressors. RESULTS: Phylogenetic analysis identified HIV-1 clades A, C and D and a recombinant A/D subtype. Grossly defective nef genes or significant changes from relevant clade reference sequences were not identified in children with delayed disease progression. CONCLUSIONS: nef sequence variations may not be common in perinatally infected African children. Further studies are warranted in HIV-1-infected subjects in settings where infection is endemic.

Adolescent↗
PubMed2004

Ectopia cordis: report of two cases.

Ectopia Cordis is a dramatic but rare cardiac anomaly with poor prognosis in most centers worldwide. This rare malformation occurs in 5.5-7.9 per million live births in the USA(1). This cardiac malformation is defined as a heart that is in an extra- thoracic position. In this article, we report two newborn infants admitted to our newborn unit with the heart beating outside the thoracic cavity. One child succumbed due to prematurity and severe cardiac malformations, while the other child successfully underwent surgical reconstruction of the ectopia cordis. She succumbed due to overwhelming sepsis one week after the surgery. Such cases have neither been reported nor treated locally.

Fatal Outcome↗
PubMed2001

Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya.

Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum beta-lactamase, which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.

Amino Acid Substitution↗
PubMed1986

Perinatal transmission of hepatitis B virus in Kenya: its relation to the presence of serum HBV-DNA and anti-HBe in the mother.

In Kenya hepatitis B virus (HBV) infection and its sequelae are common. We followed up 49 hepatitis B surface antigen (HBsAg)- positive mothers and their newborn infants for 9 months to determine the importance of perinatal transmission in the African and to relate this to the HBe and HBV-DNA status of the mother. Our study shows that perinatal transmission is relatively unimportant in Kenya and that this may be a consequence of the low levels of circulating HBV-DNA in the maternal plasma. These results imply that vaccination without hyperimmune globulin may be adequate to control HBV infection in Kenya.

DNA, Viral↗