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R Mutharasan

Publications and source records attributed to R Mutharasan.

10 recordsLinked to original sources
PubMed1999

Multi-rate nonlinear state and parameter estimation in a bioreactor.

This paper concerns real-time, multi-rate, nonlinear state and parameter estimation in a pilot-scale biochemical reactor in which cultivation of mouse-mouse hybridoma cells takes place. A multi-rate estimator is designed and implemented to estimate specific growth rate and concentrations of viable cells, total cells, glucose, glutamine, and monoclonal antibodies (MAb) in the reactor. These are estimated from frequent measurement (inferred values) of oxygen uptake rate (OUR) and infrequent and delayed measurements of the concentrations of viable cells, total cells, glucose, glutamine, and MAb. The infrequent measurements are available every 2 to 17 h with a time delay of 0.08 to 2.00 h, and OUR is inferred from dissolved oxygen concentration measurements that are available very 0.17 h. For each of the process variables, its infrequent measurement data and the profile of its estimate are presented to show the performance of the multi-rate estimator.

Animals↗
PubMed1994

Intracellular pH responses of hybridoma and yeast to substrate addition and acid challenge.

The pHi responses of hybridoma and yeast cells to substrate and external acid additions were measured using the fluorescent pHi indicator, 9-aminoacridine. The pHi change, following CCCP addition, indicated by 9AA, compared very well with that indicated by BCECF. No change in pHi was observed following glucose or glutamine additions to hybridoma cells under glucose- and glutamine-absent conditions. Also, no change in pHi was observed when glucose was added in the presence of low glutamine and when glutamine was added in the presence of low glucose. However, in the presence of amiloride, the pHi of hybridoma cells decreased following glucose addition. Intracellular pH responses of hybridoma and yeast cells to decreases in external pH, effected by acid addition, were dependent on the cellular energy state and acid type. Cells controlled their pHi more tightly under energy-poor conditions compared to energy-rich conditions, and sulfuric acid (strong acid) caused larger changes in pHi compared to pyruvic acid (weak acid). In response to acid addition under energy-rich conditions, the magnitudes of pHi increases in hybridoma were smaller in the presence of amiloride compared to that in the absence of amiloride. Further, pHi responses to a decrease in external pH were slower at submetabolic temperatures.

Acids↗
PubMed1994

Effect of taxol and diamide on shear tolerance of hybridoma and insect cells.

The role of the cytoskeletal structure on the shear tolerance of hybridoma and insect cells to laminar and turbulent shear flow field was investigated. The cytoskeletal network of cells modified by taxol and diamide, which are known to alter the stability of specific cytoskeletal components, resulted in increased cell shear tolerance to both laminar and turbulent flow fields. Plasma membrane fluidity measurements revealed that modification of microtubules by taxol did not alter the fluidity state of the plasma membrane. The increase in shear tolerance by taxol treatment is attributed to its ability to stabilize microtubules in the cytoskeleton. A low concentration of taxol, 0.05 microM, did not reduce the hybridoma cell growth rate or the monoclonal antibody production rate, but both were decreased at a higher concentration, 0.25 microM.

Animals↗
PubMed1992

Cultivation of animal cells in a reticulated vitreous carbon foam.

A reticulated vitreous carbon foam (RVCF) was used as a surface to cultivate a model anchorage-dependent animal cell line, 3T6 (mouse embryo fibroblast). This fixed-surface bioreactor provided a low-shear, chemically-inert, and reusable environment for cell growth. An external medium recirculation loop allowed aeration, nutrient monitoring, and medium replacement without disturbing the cells. Optimal flow rates for the attachment and growth phases were determined. Growth rates comparable to static (T-flask and petri dish) cultures and agitated microcarrier cultures were achieved with appropriately high medium recirculation rates. Metabolic parameters were shown to be useful indicators of cell mass, although specific glucose consumption rates were considerably higher for cultures in the RVCF reactor. Oxygen supply was shown to be the most likely limiting factor for scaleup.

Animals↗
PubMed1991

Liquid chromatographic analysis of a potential polymeric-pendant drug delivery system for peptides. Application of high-performance size-exclusion chromatography, reversed-phase high-performance liquid chromatography and ion chromatography to the evaluation of biodegradable poly[(chloromethoxytrialanine methyl ester)phosphazenes].

A novel water-soluble polymer, poly[(chloromethoxytrialanine methyl ester)phosphazene] (poly-Tame), was characterized and evaluated using high-performance size-exclusion chromatography, gradient reversed-phase high-performance liquid chromatography and ion chromatography. These novel liquid chromatographic methods were validated for application to in vitro biodegradation experiments of poly-Tame in aqueous solutions. Results from method validation experiments are presented.

Biodegradation, Environmental↗
PubMed1987

Altered Electron Flow in Continuous Cultures of Clostridium acetobutylicum Induced by Viologen Dyes.

The physiological response of Clostridium acetobutylicum to methyl and benzyl viologen was investigated. Viologen dyes at low concentrations (at levels of parts per million [micrograms per milliliter]) caused significant metabolic shifts. Altered electron flow appeared to direct carbon flow from acid to alcohol production accompanied by decreased hydrogen evolution. Reducing equivalents normally released as free hydrogen were directed toward formation of NADH which, in turn, resulted in increased alcohol production. In addition, it was shown that solvent production can take place at pH 6.3. Contrary to previous reports, butanol production appears to be independent of high levels of acetate-butyrate and glucose.

Journal Article↗
PubMedDate not supplied

Effect of serum on the plasma membrane fluidity of hybridomas: an insight into its shear protective mechanism.

We have previously shown that decreasing the concentration of fetal bovine serum (FBS) increased the fragility of a mouse hybridoma (HB-32) during agitated batch cultivation and that increasing the plasma membrane fluidity (PMF) increased the shear sensitivity during exposure to laminar flow. In this study, the effect of FBS concentration on the PMF of HB-32 was investigated. PMF was evaluated by steady-state fluorescence anisotropy (rs) of 1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene. Increasing serum concentration increased the rs of hybridomas, indicating a decrease in their PMF. The effect of cholesterol modulation on the PMF and shear sensitivity was also evaluated. Hybridomas were exposed to turbulent fluid shear after modification of PMF by cholesterol modulation. Direct cholesterol enrichment of the plasma membranes caused a decrease in the PMF and shear sensitivity, while cholesterol depletion caused an increase in PMF and shear sensitivity. Low- and high-density lipoprotein supplementation to cultures in serum-free or complete medium decreased their shear sensitivity. Lipoprotein supplementation to serum-free cultures decreased the PMF. Altogether, these results suggest that the protective mechanism of serum against hydrodynamic damage relies, at least partially, on its ability to decrease the PMF of hybridomas possibly through the transfer of cholesterol from the serum lipoproteins into the plasma membrane.

Animals↗