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R Mutters

Publications and source records attributed to R Mutters.

At least 37 records · Page 2Linked to original sources
PubMed1994

Ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract.

The phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. These strains constitute a genotypically homogeneous taxon in rRNA superfamily V, as shown by DNA-rRNA hybridization data. Determination of the 16S rRNA sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by Gherna and Woese (R. Gherna and C. R. Woese, Syst. Appl. Microbiol. 15:513-521, 1992). This new taxon is only distantly related to other members of the "flavobacter-bacteroides" phylum and is therefore given separate generic status. The DNA-DNA binding values for members of this taxon, for which we propose the name Ornithobacterium rhinotracheale, confirmed that all of the strains are highly interrelated (DNA-DNA binding values greater than 90% were measured). The G+C contents of members of this taxon are between 37 and 39 mol%. An analysis of the cellular proteins and fatty acids and classical phenotypic characteristics allowed us to distinguish O. rhinotracheale from phenotypically similar taxa, such as Riemerella anatipestifer and Capnocytophaga species. The respiratory quinone content (menaquinone 7) and carbohydrate pattern of O. rhinotracheale conform with the respiratory quinone contents and carbohydrate patterns of other members of rRNA superfamily V.

Animals↗
PubMed1993

Characterization of the family Pasteurellaceae on the basis of cellular lipids and carbohydrates.

Selected strains representing established and newly described taxa in the family Pasteurellaceae were investigated for their cellular lipid and carbohydrate composition to clarify the taxonomic significance of such features. Methylated cellular fatty acids and acetylated derivatives of the cellular carbohydrates were determined by capillary gas chromatography using a flame ionization detector. In part the carbohydrates were identified by mass spectrometry. Phospholipids were determined by thin layer chromatography, the lipoquinones by high pressure liquid chromatography. The cellular fatty acid patterns proved to be uniform with minor variations, but the separation from the Neisseriaceae and from Moraxella was possible. Also the distribution of the phospholipids was uniform within the family. The lipoquinone contents were useful for the discrimination of groups within the family not necessarily reflecting the degree of genomic relatedness. The analysis of the cellular carbohydrates resulted in a common sugar pattern with all members of the family and characteristic carbohydrate profiles discriminating groups, often to the species level. All of the cytochemical features considered were useful for the characterization of the family Pasteurellaceae.

Actinobacillus↗
PubMed1992

Occurrence of V-factor (NAD) independent strains of Haemophilus paragallinarum.

Strains of Bisgaard taxon 31, isolated from chickens in South Africa suffering from a respiratory disease with clinical symptoms and gross lesions similar to infectious coryza, showed great phenotypical similarities with Haemophilus paragallinarum infection except for NAD requirement, beta-galactosidase activity and maltose fermentation. Deoxyribonucleic acid-deoxyribonucleic acid hybridization confirmed a high level of genetic relatedness (DNA binding value, 89%) with Haemophilus paragallinarum. Guanine + cytosine content and genome size data also support the classification of taxon 31 strains within the species Haemophilus paragallinarum.

Animals↗
PubMed1991

[The genetic classification of the Pasteurella pneumotropica complex].

Pasteurella pneumotropica with its biotypes Jawetz and Heyl are the most common bacterial pathogens associated with diseases in rodents. 23 P. pneumotropica biotype Jawetz, biotype Heyl and P. pneumotropica-like rodentia isolates have been investigated phenotypically by characterization of their micromorphology and biochemical fermentation reactions. The taxonomic position within the family Pasteurellaceae has been examined by DNA:DNA hybridisation (optical method). It could be shown that P. pneumotropica biotype Jawetz represents a genus-like cluster containing several species including the V-factor dependent Haemophilus Taxon B and the avian P. pneumotropica-like organism and therefore resembles a new species of the new genus. It is concluded that the biotype Heyl of P. pneumotropica taxonomically remains as a species within the family Pasteurellaceae, however without further relationship to other known genera or genus-like groups.

Animals↗
PubMed1991

Carbohydrate patterns, cellular lipoquinones, fatty acids and phospholipids of the genus Pasteurella sensu stricto.

The carbohydrate patterns, isoprenoid quinones, fatty acids and phospholipids of the species of the genus Pasteurella sensu stricto were investigated to evaluate their taxonomic significance and their applicability for the identification of these bacteria. Forty-six representative strains of the 11 species of Pasteurella were examined. The data obtained indicated that the carbohydrate patters are species or subspecies specific and may, therefore, become an important and useful diagnostic tool. Fatty acids and phospholipids showed a feature characteristic of the members of the genus and the isoprenoid quinones exhibited a mostly genus-specific feature with remarkable quantitative differences.

Carbohydrates↗
PubMed1991

Reclassification of German, British and Dutch isolates of so-called Pasteurella multocida obtained from pneumonic calf lungs.

The taxonomic relationship of 131 strains previously identified as Pasteurella multocida obtained from calf pneumonia in West Germany, United Kingdom and Netherlands was investigated by extended phenotypic and limited genotypic characterization. Twenty-four strains were classified as P. multocida ssp. multocida, 15 strains as P. avium biovar 2 and 13 strains as P. canis biovar 2. Sixty-five and five strains were tentatively classified as ornithine negative P. multocida ssp. multocida and P. multocida ssp. septica, respectively. Genetic investigations showed that ornithine negative strains of P. multocida were related on species level. Less genomic binding was found between an ornithine negative strain of P. multocida ssp. septica and the type strains of the three subspecies of P. multocida. The taxonomic position of ornithine negative strains of P. multocida is still under investigation. The taxonomic position of the remaining nine strains is uncertain underlining the need for genotypic characterization within the genus Pasteurella to aid in defining single species by phenotypic tests.

Animals↗
PubMed1991

Some unusual members of the family Pasteurellaceae isolated from human sources--phenotypic features and genomic relationships.

Twelve human isolates exhibiting the properties of the family Pasteurellaceae but phenotypically deviating from established species, or resembling species of animal origin that are only rarely reported to occur in human materials, were checked for their identities by DNA-DNA hybridization. The collection consisted of one strain of Actinobacillus lignieresii, two strains of Actinobacillus hominis (mannose-positive), two hitherto undescribed Actinobacillus or Actinobacillus-like species, [Pasteurella] haemolytica biovar T, CDC group HB-5 (initially oxidase and indole-negative), a new species in the [Haemophilus] aphrophilus [Haemophilus] segnis group, a new sucrose-negative and gas-producing Pasteurella-like species, and three strains of Bisgaard's Pasteurella-like taxon 16. Some diagnostically useful features of these unusual human Pasteurellaceae are described.

Animals↗
PubMed1991

Capillary gas chromatography of cellular carbohydrates as a means for the differentiation of fastidious, slow-growing or anaerobic gram-negative bacteria--a review.

The phenotypic differentiation of microaerophilic or anaerobic Gram-negative bacteria of human origin is traditionally based on the analysis of metabolic characteristics. Due to outstanding growth requirements of many kinds of parasitic bacteria, e.g. of the facultatively and strictly anaerobic members of the human periodontal flora, conventional biochemical tests are time-consuming and sometimes lead to doubtful results. Commercial diagnostic test kits often produce misidentifications because of insufficient databases or inappropriate test combinations. In this situation, cytochemical methods can provide an alternative. Especially in the case of Gram-negative bacteria which contain considerable amounts of carbohydrates in their cell wall lipopolysaccharide layers, capillary gas chromatography of peracetylated carbohydrate derivatives prepared from bacterial whole cell hydrolysates proved to be useful to characterize individual species, and sometimes even subspecies. As soon as a pure culture is available, the derivatization of the cellular carbohydrates to their peracetylated aldononitriles and peracetylated O-methyloximes, respectively, needs only about four hours and the gas chromatographic spectra are easy to evaluate.

Alcaligenes↗
PubMed1991

[The importance of Pneumocystis carinii infection in patients with acute respiratory distress syndrome].

Each year we treated approximately 40 patients with adult respiratory distress syndrome (ARDS) in our department of anaesthesiology. In these cases we found with bronchoalveolar lavage followed by microbiological examination, a high incidence (40 per cent) of pneumocystis carinii contamination. An infection with Pneumocystis carinii deteriorates the clinical course of ARDS. Effective drugs were the combination of trimethoprim and sulfamethoxazole. Under this therapy we always saw a decrease of infiltrations in the chest-x-ray as well as an improved gas exchange. No relapse was seen in any case.

Adolescent↗
PubMed1991

Rapid differentiation of the species of the genus Bacteroides sensu stricto by capillary gas chromatography of cellular carbohydrates.

Whole-cell hydrolysates of Bacteroides fragilis, the type species of the genus Bacteroides Castellani and Chalmers 1919, and the genetical closely related species B. vulgatus, B. ovatus, B. eggerthii, B. distasonis, B. uniformis, B. thetaiotaomicron, B. stercoris, B. merdae, and B. caccae were used to determine characteristic carbohydrate patterns by capillary gas chromatography. On the basis of the chemical derivatization of the carbohydrates seven characteristic peaks for peracetylated aldononitriles and nine characteristic peaks for peracetylated o-methyloximes were selected from the carbohydrate fingerprints of the reference strains to prepare a dichotomous identification key. The classification of an unknown strain supposed to belong to the formerly called 'Bacteroides fragilis group' is possible with this key. Some of the advantages of the technique were that the identification of Bacteroides fragilis-like strains requires only 4-5 h after primary isolation and that the bacteria can be exposed to oxygen because viability of the organisms is not necessary. Sophisticated anaerobic techniques can therefore be avoided for identification.

Bacterial Typing Techniques↗
PubMed1990

Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae.

We performed hybridizations between labeled rRNAs from seven representative members of the family Pasteurellaceae and from three other taxa on the one hand and DNAs from 53 strains known or presumed to belong to the Pasteurellaceae on the other hand. The members of the Pasteurellaceae are most closely related to members of the Enterobacteriaceae, the Vibrionaceae, the Aeromonadaceae, and the genus Alteromonas. The family Pasteurellaceae is very heterogeneous. There are at least seven rRNA branches. Several organisms with the same genus name are dispersed over the entire dendrogram. The "Histophilus ovis," [Haemophilus] ducreyi, [Actinobacillus] actinomycetemcomitans, and [Haemophilus] aphrophilus rRNA branches are separate and quite remote from the three authentic genera in this family; this might justify eventual later separate generic status. DNA-rRNA hybridization with suitable, labeled rRNA probes is an excellent method to establish whether an organism belongs in the Pasteurellaceae; e.g., some strains of Bisgaard's taxa 7, 13, and 16 and of the gas-producing "SP" group certainly belong in this family, whereas three bovine lymphangitis organisms (strains NCTC 10547, NCTC 10549, and NCTC 10553), [Haemophilus] piscium ATCC 10801T (T = type strain), and [Pasteurella] piscicida ATCC 17911 belong in the Enterobacteriaceae, the Aeromonadaceae, and the Vibrionaceae, respectively.

Actinobacillus↗
PubMed1989

Deoxyribonucleic acid relatedness and phenotypic variation among human isolates of Eikenella corrodens.

The type strain of Eikenella corrodens (Eiken 1958) Jackson and Goodman 1972 and eleven epidemiologically independent clinical isolates recovered from periodontal locations, putrid wounds, abscesses, and bacteraemias were investigated for their genomic relationships by DNA-DNA hybridization with the renaturation method, genome molecular complexity, DNA base composition and some phenotypic features. The bacterial strains studied were interrelated at or above the 80% DNA binding level, their chromosomal DNAs exhibiting a mean molecular mass of 1.7 x 10(9) daltons and a mean guanine plus cytosine content of 55.1 mol%. Variations in colonial morphology, hemolytic activity on sheep blood agar, reduction of nitrates, oxidation of carbohydrates, lipase, leucine, valine, and cystine aminopeptidase and acid phosphatase activities occurred among closely interrelated strains. The definition of the species and current identification keys must be emended accordingly.

Bacteroides↗
PubMed1989

Rapid identification of Haemophilus influenzae serovar b by gas liquid chromatography using carbohydrate fingerprints.

Carbohydrates from whole-cell hydrolysates of 18 strains of the species Haemophilus influenzae (5 strains belonging to serovar b) were analysed by gas-liquid chromatography. The identity of the carbohydrate components was confirmed by comparison with the retention times of reference sugars and by gas chromatography mass spectrometry. Evidence was obtained that Haemophilus influenzae serovar b can easily be identified by the presence of one large peak representing ribitol. The method described can be routinely applied in bacteriological laboratories equipped with a gas chromatograph. It gives results within approximately 4 h, it is reproducible and easy to perform. Even single colonies isolated directly from agar plates can be used for analysis without further subculturing.

Carbohydrates↗
PubMed1989

Characterization of some previously unclassified Pasteurellaceae isolated from hamsters.

Bacteria isolated from purulent processes on the jaws of European hamsters (Cricetus cricetus) and from intestinal inflammatory processes in Syrian hamsters (Mesocricetus auratus), bred as laboratory animals have been shown to be phenotypically similar but not identical with Pasteurella pneumotropica. Deoxyribonucleic acid (DNA)-DNA hybridization studies indicate that with one exception, the strains represent two new species of the family Pasteurellaceae. In the absence of a close genomic relatedness to members of the genera Actinobacillus or Pasteurella or allied organisms, however, the two new taxa are described without any formal designation. The one exception was identified as Actinobacillus capsulatus, a species not previously isolated from hamsters.

Actinobacillus↗
PubMed1987

V-factor dependent strains of Pasteurella multocida subsp. multocida.

At bacteriological examination of swine lungs with pneumoniae due to Mycoplasma hyopneumoniae, two strains of pyridine nucleotide (V-factor) requiring bacteria were isolated. They exhibited a pattern of biochemical reactions fitting with that of Pasteurella multocida subsp. multocida. Deoxyribonucleic acid (DNA): DNA hybridization with these isolates and Pasteurella multocida subsp. multocida showed that they were V-factor requiring strains of this species. Preliminary investigations on the qualitative and quantitative V-factor requirements have been performed.

Animals↗
PubMed1986

Characterization of some previously unclassified "Pasteurella" spp. obtained from the oral cavity of dogs and cats and description of a new species tentatively classified with the family Pasteurellaceae Pohl 1981 and provisionally called taxon 16.

The taxonomic relationship of 23 unclassified canine and feline strains of Pasteurellaceae and five strains received as Pasteurella spp. or Haemophilus influenzae-murium was investigated by phenotypic and genetic characterization. Eleven strains were classified with four recently described species of genus Pasteurella sensu stricto. Fourteen canine and feline strains formed a homogeneous group, tentatively designated taxon 16. Both phenotypic characters and mol% G + C in DNA and genome size indicate classification of taxon 16 with genus Pasteurella. DNA/DNA hybridizations, however, failed to locate taxon 16 on genus level with accepted or proposed species of the family Pasteurellaceae Pohl 1981. Two additional species obtained from rats and mice remained unclassified, in addition to a human isolate obtained from a dog-bite. The necessity of detailed phenotypic characterization within the family Pasteurellaceae Pohl 1981 needs to be stressed.

Animals↗
PubMed1986

Re-investigations of selected bovine and ovine strains previously classified as Pasteurella haemolytica and description of some new taxa within the Pasteurella haemolytica-complex.

The taxonomic relationship of 116 isolates of Pasteurella haemolytica was re-investigated by conventional phenotypic characterization. Seventy-nine characters were examined. Ninety strains were classified with previously described biovars of P. haemolytica. The remaining strains made up at least six new biogroups within the P. haemolytica-complex. "Key-characters" separating these groups included ornithine, L(+)arabinose, D(-)sorbitol, cellobiose, beta-glucosidase, glycosides and alpha-fucosidase. The taxonomic significance of these characters is uncertain. Determinations of genome size, mol% G + C in DNA and DNA:DNA hybridizations are in progress to obtain further information on the taxonomic significance of the present findings.

Animals↗