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Biomedical subjects

R Neumann

Publications and source records attributed to R Neumann.

At least 181 records · Page 10Linked to original sources
PubMed1981

Cloned fragments of human adenovirus type-12 DNA.

The following restriction endonuclease fragments of human adenovirus type 12 (Ad12) DNA have been cloned in plasmid or bacteriophage lambda vectors using standard protocols: the EcoRI-A*, -B, -D, -E, and -F fragments, the BamHI-B, -C, -D, -F, -G, -H, and -I fragments, the HindIII-F and -I fragments, and the PstI-A, -D, -F, -G, and -H fragments. The EcoRI-A* fragment comprises the right terminal 5 kb of Ad12 DNA including the terminal 143 bp.

Adenoviruses, Human↗
PubMed1981

Integration of adenovirus type 2 DNA at a limited number of cellular sites in productively infected cells.

Several experimental findings supported the notion that adenovirus type 2 DNA is covalently linked to cellular DNA in productively infected human cells. Although the significance of this linkage for the process of viral replication is unknown, the recombination of viral DNA with host DNA may simply reflect the efficacy of the recombination systems in mammalian cells. In this report, evidence is presented for the existence of selective sites of recombination between viral and host DNAs. These sites are presumably located in repetitive sequences of cellular DNA. All parts of the viral genome appear to be represented in the recombinant form.

Adenoviruses, Human↗
PubMed1980

DNA methylation and viral gene expression in adenovirus-transformed and -infected cells.

The level of DNA methylation in adenovirus type 2 (Ad2) and type 12 (Ad12) DNA was determined by comparing the cleavage patterns generated by the isoschizomeric restriction enzymes HpaII and MspI. As previously reported virion DNA of Ad2 and Ad12 is not methylated. Parental or newly synthesized Ad2 DNA in productively infected human KB or HEK cells is not methylated either, nor is the integrated form of Ad2 DNA in productively infected cells. Hamster cells and Muntiacus muntjak cells are abortively infected by Ad12. We have not detected methylation of Ad12 DNA in hamster or Muntiacus muntjak cells. An inverse correlation between the level of methylation and the extent of expression of viral DNA in Ad12-transformed hamster cells has been described earlier. A similar relation has been found for the EcoRI fragment B of Ad2 DNA which is not methylated but is expressed as the Ad2 DNA-binding (72K) protein in the Ad2-transformed hamster line HE1. Conversely, the same segment is completely methylated in lines HE2 and HE3, and there is apparently no evidence for the expression of the 72K protein in these cell lines.

Adenoviruses, Human↗
PubMed1980

Increased creatine kinase BB activity in rat plasma induced by hypoxia.

Rats show an elevated creatine kinase (CK) activity in plasma after a 20-hour exposure to hypoxia. The increase of CK activity is essentially caused by CK-BB activity attaining its maximum at the 3rd day after exposure to hypoxia. The possibility of establishing a potential brain damage by determining CK-BB activity in plasma is discussed.

Animals↗
PubMed1980

Lecithin: cholesterol acyltransferase activity, HDL-cholesterol and apolipoprotein A in serum of patients undergoing chronic haemodialysis.

In comparison with a control group, significantly lower lecithin:cholesterol acyltransferase activities and HDL-cholesterol values were found in the serum of patients undergoing chronic haemodialysis, while apolipoprotein A concentration remained unchanged. Reduced activity of lecithin:cholesterol acyltransferase is an indication of the abnormal cholesterol metabolism in haemodialysed patients and could be, in addition to other factors, an explanation for the high incidence of cardiovascular diseases in this patient group.

Apolipoproteins↗
PubMed1980

Viruses as tools for studies on the molecular biology of mammalian cells.

1. Multiple copies of intact adenovirus type 12 (Ad12) DNA are integrated into the DNA of Ad12-transformed hamster and Ad12-induced rat brain tumor cells. Free viral DNA is not present in the Ad12-transformed lines investigated. 2. Only few sites of integration are found in Al2-transformed hamster and Ad12-induced rat brain tumor cells. Integration may have occurred into repetitive cellular sequences at selective sites. These sites may be different in different cell lines, however, none of these sites has been analyzed in sufficient detail. 3. Three lines of Ad12-induced rat brain tumor cells exhibit identical patterns of integration. These lines have been derived from three brain tumors in one animal and may have evolved from the same transformed cell. 4. In Ad12-induced rat brain tumor cells early and late segments of the viral genome are expressed as polysome-associated messenger RNA. 5. In human cells productively infected with adenovirus type 2 (Ad2), a large number of viral genome copies are linked to cellular DNA early postinfection. There are only a few sites of recombination (illegitimate?) which probably lie in repetitive cellular DNA sequences. The functional significance of this frequent recombination is unknown.

Adenoviridae↗
PubMed1979

Diethylmesoxalate hydrate, a new irreversible inhibitor of cholinesterases.

Bovine erythrocyte acetylcholinesterase and human plasma cholinesterase are irreversibly inhibited by diethylmesoxalate hydrate, the inhibition potency being comparable to that of certian insecticidal organophosphates and carbamates. Insect cholinesterases, however, appear to be much less affected by diethylmesoxalate hydrate. The compound was also found to inhibit the hydrolysis of paraoxon by rabbit plasma A-esterase, but in a reversible mode.

Animals↗
PubMed1979

Creatine kinase isoenzyme BB in serum of healthy adults and children.

The activity of the creatine kinase isoenzyme BB was determined in the serum of 26 healthy adults and 31 children. The isoenzyme BB could be proved as a normal component in the human serum. In the adults examined, an activity of 0.56 +/- 0.16 I.U./l (x +/- S.D.) was determined. The activity of creatine kinase isoenzyme BB in the serum does not depend on sex but is subject, however, to a strong age dependence. Only at an age of more than 18 years, isoenzyme BB activities adjust to those of adults.

Adult↗
PubMed1978

Lecithin: cholesterol acyltransferase activity in patients with chronic liver diseases and positive LP-X tests.

Lecithin: cholesterol acyltransferase activity has been measured in serum from patients with various liver disorders and positive LP-X tests and from healthy subjects. Statistical analysis indicated that lecithin: cholesterol acyltransferase activity provided no help in the discrimination between the persons of both groups although the mean activities differed significantly.

Chronic Disease↗