Biomedical subjects

R O Pedraza

Publications and source records attributed to R O Pedraza.

3 recordsLinked to original sources

PubMed2002

In-well cell lysis technique reveals two new megaplasmids of 103.0 and 212.6 MDa in the multiple plasmid-containing strain V517 of Escherichia coli.

AIMS: Identification of two new plasmids in the multiple plasmid-containing strain V517 of Escherichia coli. METHOD AND RESULTS: By using an in-well mild cell lysis technique suitable for megaplasmids observation, two plasmids of 103.0 and 212.6 MDa were detected in the multiplasmid-containing E. coli V517. CONCLUSIONS: The two new megaplasmids that were completely overlooked when standard disruptive procedures were used, can now be added to the list of eight plasmids with molecular size from 1.36 to 35.84 MDa reported earlier. SIGNIFICANCE AND IMPACT OF THE STUDY: This finding allows to use the strain V517 not only as a size reference of small and moderately large plasmids but as a size reference of megaplasmids as well.

Bacteriological Techniques↗

PubMed1984

Tuberculosis in Cuba.

Tuberculosis is a significant public health problem in developing countries, despite the fact that the means for effective control have been available for more than 35 yr. Cuba is a developing country that has achieved control of tuberculosis comparable to that of the United States. The history of tuberculosis control in Cuba prior to 1959 is reviewed. The achievement of effective control during the past 24 yr results from the implementation of ambulatory, supervised chemotherapy at the primary care level and the priority set by the government. Case-finding strategies, chemotherapeutic regimens, prevention, and economic implications are discussed. A wider application of the successful Cuban experience is possible by providing an overall improvement in living conditions, a significant but modest investment in human resources, and a political commitment to improved health care.

Adolescent↗

PubMedDate not supplied

[Azospirillum amazonense: its presence in the sugar cane area of the province of Tucumán].

We confirm the presence of Azospirillum amazonense in the sugar cane area of the Province of Tucumán. Several strains were isolated from sugar-cane roots in different places. Phenotypic and genotypic characteristics coincided with those described for this species. This new one is being added to the other two species, A. brasilense and A. lipoferum, already reported.

Argentina↗