Biomedical subjects
Publications and source records attributed to R Odedra.
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
The presence of circulating anti-heart IgG and IgM autoantibodies was assessed by indirect immunofluorescence and by probing Western blot transfers of normal canine myocardial proteins separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis with samples of sera from dogs with dilated cardiomyopathy (n = 27) and from healthy control dogs (n = 20). No difference was demonstrated between the two groups using either method. Sera taken from the affected dogs at three monthly intervals throughout the course of the disease showed no change at all in the pattern of reactivity. The results of this study suggest that indirect immunofluorescence and the probing of Western blot transfers of a crude preparation of normal canine myocardium with canine sera will demonstrate too many apparent autoantigens for the technique to identify unique, disease-associated autoantibodies.
A number of substances have been proposed for the role of angiogenesis factors. Many of these are of protein origin and are therefore amenable to the tools of the molecular biologist. However a number of low molecular weight angiogenesis factors are emerging as important initiators and/or cofactors of neovascularization. Of these a number are known to stimulate angiogenesis indirectly, possibly through an inflammatory response. Some putative angiogenic factors stimulate microvessel endothelial cells nonspecifically, also causing migration and proliferation of large vessel cells. Others are specific for microvessel cells either for stimulating migration, proliferation or both. The nature and action of the low molecular weight factors in vivo and in vitro are reviewed.
Stimulation of microvessel endothelial cells grown on collagen gels by endothelial cell stimulating angiogenesis factor was confirmed (1). The potent endothelial cell growth stimulating activity of basic fibroblast growth factor has also been demonstrated for cells grown on collagen gels. However, the growth factor activity of basic fibroblast growth factor towards microvessel endothelial cells was almost eliminated when experiments were carried out in the presence of diafiltered foetal calf serum, but was partially restored by the addition of endothelial cell stimulating angiogenesis factor to medium containing the diafiltered serum. In contrast to these observations, foetal skin fibroblasts were stimulated by basic fibroblast growth factor when grown in medium with diafiltered serum, and this stimulation was not modified by the presence of endothelial cell stimulating angiogenesis factor.
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