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Biomedical subjects

R P Fule

Publications and source records attributed to R P Fule.

At least 19 recordsLinked to original sources
PubMed1991

Nosocomial infection due to salmonella senftenberg (case report).

Besides various clinical manifestations and made of spread of Salmonella infections, isolation of S. senftenberg a rare serotype from patients hospitalised for surgical procedures indicates nosocomial infection. A prolonged stay in hospital with surgical trauma facilitated the organism to cause infection in the compromised host.

Adult↗
PubMed1990

Drimarene brilliant blue--a better pre-stain in protein separation and visualization.

Prestaining of human serum proteins with a new reactive dye Drimarene Brilliant Blue (DBB), was standardized employing 940 separations and examining 30 variables. Under the critical condition, the serum and the soluble dye (0.1 g/100 ml in working Tris-glycine buffer, pH, 8.3), was mixed in equal proportion, conjugate warmed at 40 degrees C for 2 hr and a 30 microliter of the sample electrophoresed by disc electrophoresis. The method when compared with prestaining by Remazol Brilliant Blue (RBB) and postelectrophoretic staining by Amido Black (AB) in 50 normal sera, revealed that the discs stained with DBB were intense and well defined and appeared in 2 hr on a sparkingly clear gel. Quality of resolution was better than RBB and AB. Protein bands eluted from the DBB prestained gels retained their immunoreactivity. The dye-protein complex of albumin and transferrin produced high-titre monospecific antisera in rabbits.

Anthraquinones↗
PubMed1990

Antibiotic resistance, haemagglutination type & haemolysin production in relation to serogroups of uropathogenic Escherichia coli.

A total of 56 urinary isolates of Esch. coli were characterised according to serotype, haemagglutination (HA) type, production of beta haemolysin and antibiotic resistance pattern. Forty five strains were serotyped with prevalence of 057 followed by other serotypes. Eleven different Esch. coli serotypes were found to have mannose resistant haemagglutinating property (MRHA) and just five strains showed haemolysin production (Hly+). Multidrug resistance was common with preponderance of ampicillin, co-trimoxazole and tetracycline resistance. No correlation between serogroup, HA type, haemolysin production and antibiotic resistance was found.

Bacteriuria↗
PubMed1990

Incidence of Gardnerella vaginalis infection in pregnant & non-pregnant women with non-specific vaginitis.

The occurrence of G. vaginalis in patients with non-specific vaginitis (NSV) was studied. Of the 200 women with vaginitis screened, 84 were diagnosed to have non-specific vaginitis. G. vaginalis was isolated from 33 (39.28%) patients either alone or in combination with other organisms, while one of the healthy controls with no vaginal discharge yielded G. vaginalis on culture. The nature of vaginal discharge could not be correlated with the type of infection. Clue cells and amine test gave inconsistent results in the presence of G. vaginalis infection. A varying susceptibility pattern of G. vaginalis was observed against various antimicrobials, metronidazole (95%) and gentamicin (75%) showing highest sensitivity.

Female↗
PubMed1990

Cholera epidemic in Solapur during July-August, 1988.

Of the 77 Vibrio cholerae isolated during July to September, 1988, 76 were El Tor vibrios serotype Ogawa, 68 belonging to T2 phage (eight strains untypable). Peak incidence was noted in the month of August, 1988. Haemolytic and non-haemolytic colony variants of El Tor V. cholerae were encountered. All strains showed resistance to one or more antibiotics. No fatality was reported during the epidemic. The epidemiological data collected over the past three years (1986-88) show that cholera is prevalent in the local environment.

Cholera↗