Biomedical subjects

R P Howe

Publications and source records attributed to R P Howe.

9 recordsLinked to original sources

PubMed1992

Magnetic bead purification of M13 DNA sequencing templates.

A method for the preparation of multiple M13 DNA sequencing templates is described. No phenol extraction is required and the procedure can be carried out in Eppendorf tubes or 96-well microtiter plates. Starting with a phage supernatant, the entire procedure is carried out in the same reaction vessel and all separation steps are based on a novel application of magnetic bead separation. The design of a 96-well magnetic separator is presented and the application of the method for large-scale sequencing is discussed.

Bacteriophages↗

PubMed1990

A comparison of the Herbst and Fränkel appliances in the treatment of Class II malocclusion.

This study investigated the treatment effects produced by two types of functional appliance, one primarily tooth-borne (the Herbst appliance) and one primarily tissue-borne (the FR-2 of Fränkel). Serial cephalometric radiographs from 45 patients who had been treated with the acrylic splint Herbst appliance and from 41 patients who had been treated with the FR-2 appliance were compared with serial radiographs of 21 untreated persons with Class II malocclusions. Treatment effects were identified through the use of a conventional cephalometric analysis. The results of this study indicated that both appliances had influenced the growth of the craniofacial complex in treated persons. Significant skeletal changes were noted in both treatment groups, with both groups showing an increase in mandibular length and in lower facial height, as compared with controls. Greater dentoalveolar treatment effects were noted in the group wearing the tooth-borne functional appliance than in those wearing the tissue-borne appliance.

Activator Appliances↗

PubMed1988

Clinical management of the acrylic splint Herbst appliance.

This article describes one variation in Herbst appliance design--the acrylic splint Herbst. Topics discussed include early fixed appliance treatment before Herbst therapy, impression taking, bite registration, and evaluation of appliance fabrication. The specifics of appliance delivery, including bonding of the maxillary appliance when indicated, are also discussed as are the techniques of appliance advancement and removal.

Acrylic Resins↗

PubMed1987

Lower premolar extraction/removable plastic Herbst treatment for mandibular retrognathia.

The patient was a 12-year-old girl with a Class I malocclusion characterized by maxillary arch width constriction, dental crowding, and mandibular retrognathia. Treatment goals related to the chief compliant were anterior mandibular repositioning, maxillary arch expansion, and dental alignment. Because the preexisting overjet was minimal and prevented adequate anterior repositioning of the mandible, lower first premolar extraction was recommended to increase the overjet and allow greater anterior repositioning. Treatment was divided into four stages: (1) extraction of mandibular first premolar teeth, alignment, space closure, and an increase in the overjet, (2) anterior mandibular repositioning with a removable plastic Herbst appliance, (3) arch alignment and detailing of the occlusion with full fixed appliances and a tooth positioner, and (4) retainers. At the end of treatment, the patient had an acceptable dental alignment with a Class III molar relationship and an improvement in facial appearance.

Activator Appliances↗

PubMed1987