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Biomedical subjects

R P Human

Publications and source records attributed to R P Human.

9 recordsLinked to original sources

Evaluation of swab transport systems against a published standard.

BACKGROUND: Before the publication of the M40-A standard by the United States National Committee for Clinical Laboratory Standards (NCCLS), no quality control data for swab transport systems (STSs) had been available. AIMS: To compare three commercially available STSs against the published standard to ascertain bacterial survival after a set holding time. METHOD: Charcoal and non-charcoal containing swabs were inoculated with standard volumes and numbers of a range of bacteria commonly isolated from clinical material. Bacterial counts were taken at time zero, six, 24, and 48 hours, with the results being compared against the NCCLS standard. RESULTS: The standard clearly differentiated between the three STSs tested, with one product being superior to the other two products in providing better survival. CONCLUSION: The standard now provides manufacturers and users of STSs with meaningful data to provide, purchase, and compare an STS for routine clinical use.

Bacteria↗

Identification of Gardnerella vaginalis with the API 20 Strep system.

A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.

Female↗

Contamination of hypodermic needles in anaesthetic practice.

The study was undertaken to define and compare the incidence of bacterial contamination on opening three different types of hypodermic needle package. Four user groups were studied (anaesthetists, intensive care nurses, operating department assistants and medical laboratory scientific officers). Bacterial contamination was shown to occur and to be related to the type of needle packaging, the ratio of contamination incidence being similar in all four user groups. The results emphasize the importance of a careful opening technique when using sterile packaged hypodermic needles.

Anesthesiology↗