Shigellosis complicated by perforated appendix.
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Appendicitis↗
Biomedical subjects
R P Shapiro
Publications and source records attributed to R P Shapiro.
Childhood protein-losing enteropathy associated with Helicobacter pylori infection.
Helicobacter pylori was found in a gastric biopsy specimen of a child with protein-losing gastropathy. Through erythromycin therapy, clinical and ultrasonographic recovery was associated with the disappearance of the pathogen on repeated biopsy. The association of H. pylori with protein-losing gastropathy has been reported only once in children, to our knowledge. Although the causative correlation between the pathogen and the disease has not been proven yet, we suggest that future cases of protein-losing gastropathy be studied for the presence of H. pylori and followed up by ultrasonographic investigation and urea breath test. Appropriate treatment should be given to suspected cases.
Biopsy↗
Production and characterization of monoclonal antibodies to bovine beta 2-microglobulin.
In an attempt to isolate monoclonal antibodies specific for bovine lymphocytes, spleen cells from mice immunized with bovine lymphocytes were fused with the mouse myeloma cell line SP-2/0. The resulting hybridoma cell lines were tested for reactivity with bovine lymphocytes, polymorphonuclear neutrophils, RBC, gamma-globulin, kappa-casein, beta-casein, alpha-S1-casein, and beta 2-microglobulin (beta 2m) and with beta 2m from rabbits, goats, and human beings. None of the clones secreted anti-bovine lymphocyte-specific antibody. However, 4 secreted monoclonal antibodies to bovine beta 2m. They also reacted with beta 2m from rabbit, goat, and human being. One monoclonal antibody also was found to be reactive with bovine immunoglobulin. Monoclonal antibodies to beta 2m could serve as a tool to (1) explore the homology of the beta 2m molecule among various species, (2) examine the relationship of beta 2 m with the constant region of the immunoglobulin molecule, (3) quantitate bovine beta 2m in various body fluids and major histocompatibility antigens on cell surfaces, (4) help characterize those antigens in cattle, and (5) be used for tissue typing of those antigens.
Animals↗
Production of monoclonal antibodies to bovine leukocyte cell-surface components.
Fusion of murine myeloma cells with syngeneic spleen lymphocytes has led to development of hybridomas secreting antibodies. Hybrid cells retain the immortality and clonability of myeloma parents as well as the antibody-producing property of lymphocytes. Specificity of monoclonal antibody produced is based on the one lymphocyte-one antibody phenomenon and represents the most effective process for producing specific antisera. spleen cells from mice immunized with desired antigen are hybridized with nonsecreting mouse myeloma cells. Resulting hybrids are cloned and culture fluids tested for specific antibody activity to the antigen. Positive clones are cultivated in vitro and injected into mice for monoclonal antibody production. This technology has been extended to the bovine species to obtain monoclonal antisera to immunoglobulins and cell-surface components of leukocytes for study of mammary gland immunity. Recent progress in monoclonal research has led to interspecific fusion of murine myelomas with bovine lymphocytes, resulting in hybridomas that produce monoclonal bovine immunoglobulins. Monoclonal antibodies will be useful in investigations applicable to bovine research including purification of immunoglobulins, determining immunoglobulin concentration in colostrum and milk, reference reagents for bovine serology, antibody localization in tissue, gene sequencing, characterizing histocompatibility antigens, distinguishing and quantitating cell types in blood, milk, and udder tissue, and elucidating role of cell subpopulations in the immune response.
Animals↗
Outpatient pediatric surgery in a developing country.
A total of 5,522 outpatient surgical procedures were performed at the Children's Hospital in Kingston, Jamaica, West Indies, between 1968 and 1973. This included 2,662 general surgical procedures. There were no deaths or major complications. Parents participated actively in the preoperative and postoperative care. Outpatient surgery can be a safe, inexpensive, and effective means of health care delivery. It is of special value in developing countries with limited facilities for inpatient care.
Ambulatory Surgical Procedures↗