Biomedical subjects

R Pöltl

Publications and source records attributed to R Pöltl.

4 recordsLinked to original sources

PubMed1998

The short tandem repeat locus D3S1359.

Short tandem repeats (STRs) are used by many laboratories throughout the world performing paternity testing or criminal casework. Nevertheless, many of the established STRs have obvious disadvantages such as low number of common alleles (e.g., hTPO, THO1) or alleles with frequencies of nearly 50% (e.g., hTPO, FES). In this paper the new STR locus D3S1359 is described. In a population study which was carried out on 136 unrelated individuals from southwestern Germany, we have detected 17 different alleles. The most common allele was allele 13 (204 bp) with a frequency of 18.8%. Eight further alleles have frequencies higher than 5%. With a heterozygozity index of 90% and 60 different genotypes, D3S1359 has shown to be a highly polymorphic and informative marker. Sequencing data of this STR locus revealed further variation.

Alleles↗

PubMed1997

Typing of the short tandem repeat D8S347 locus with different fluorescence markers.

The short tandem repeat (STR) locus D8S347 was analyzed by capillary electrophoresis. Sequencing data and a population study of 203 individuals from a southwestern German population are presented. We detected 12 different alleles, 340-388 bp in length, and found 40 different genotypes. The heterozygosity index was 85.7%. Futhermore, we investigated the consequences of different fluorescent dyes, namely 6-FAM, HEX, and ROX, on the ABI-calculated fragment sizes of defined (i.e., sequenced) alleles (348-376 bp in length). 6-FAM-labeled fragments appear to be smaller than the corresponding HEX- or ROX-labeled fragments. On average, 6-FAM-labeled fragments differ by 3.52 bp from the sequencing data, HEX-labeled ones by 2.04 bp, and ROX-labeled ones by 1.42 bp. Generally, small alleles differ less from the expected sequencing data than larger ones.

Chromosome Mapping↗

PubMed1996

Comparison of German population data on the apoB-HVR locus with other Caucasian, Asian and black populations.

A population study of 505 unrelated individuals from Southwestern Germany was carried out on the 3'-apoB hypervariable region (HVR). After amplification via polymerase chain reaction (PCR) and agarose gel electrophoresis, 15 different alleles and 47 genotypes were observed. The most common alleles were hypervariable elements (HVE) 37 and 35 with an allele frequency of 0.374 and 0.244, respectively. The heterozygosity index was calculated to be 78.4%. Allele frequencies of this study are compared with results from other databases obtained from a French, a Spanish, an Asian and an American (Black) population.

Alleles↗

PubMedDate not supplied

The short tandem repeat loci hTPO, THO1 and FGA.

This paper focuses on the three well-established short tandem repeats HumhTPO (human thyroid peroxidase gene), HumTHO1 (human tyrosine hydroxylase gene) and HumFGA (human alpha fibrinogen gene). We present sequencing data which include the sequences of all the different primer pairs used so far, as well as results from a population study which was carried out on an American Black and a southwestern German population. All data are compared with previously published studies. A test for homogeneity confirmed that non-European populations possess different genetic pools for each of the three loci under investigation.

Base Sequence↗