PubMed Health⌕ Search

Biomedical subjects

R Pates

Publications and source records attributed to R Pates.

5 recordsLinked to original sources
PubMed1991

The prevention of HIV infection from injecting drug use--a review of health promotion approaches.

This review examines the prevention of HIV infection among injecting drug users through the framework provided by the Ottawa Charter on Health Promotion. Among the key issues considered are the problems of achieving the changes in policy and service direction needed to prevent HIV infection. The role of the media in influencing public opinion is examined, together with the potential for traditional educational approaches to achieve safer practices among injecting drug users. The authors conclude that a better integrated and more comprehensive approach to HIV prevention may be needed for substantial progress to occur. A proactive response utilising outreach work is recommended for drug services, and health workers together with drug service professionals are encouraged to act as advocates in gaining public and political support for change.

HIV Infections↗
PubMed1979

Cell surface location of simian virus 40-specific proteins on HeLa cells infected with adenovirus type 2-simian virus 40 hybrid viruses Ad2+ND1 and Ad2+ND2.

HeLa cells infected with the nondefective adenovirus type 2-simian virus 40 hybrid viruses Ad2+ND1 or Ad2+ND2 were analyzed for cell surface location of the SV40-specific hybrid virus proteins by indirect immunofluorescence microscopy. Two different batches of sera from SV40 tumor-bearing hamsters, serum from SV40 tumor-bearing mice, or two different antisera prepared against purified sodium dodecyl sulfate-denatured SV40 T-antigen, respectively, were used. All sera were shown to exhibit comparable T- and U-antibody titers and to specifically immunoprecipitate the SV40-specific proteins from cell extracts of Ad2+ND2-infected cells. Whereas analysis of living, hybrid virus-infected HeLa cells did not yield conclusive results, analysis of Formalin-fixed cells resulted in positive cell surface fluorescence with both Ad2+ND1- and Ad2+ND2-infected HeLa cells when antisera prepared against sodium dodecyl sulfate-denatured SV40 T-antigen were used as first antibody. In contrast, sera from SV40 tumor-bearing animals were not or only very weakly able to stain the surfaces of these cells. The fact that the tumor sera had comparable or even higher T- and U-antibody titers than the antisera against sodium dodecyl sulfate-denatured T-antigen but were not able to recognize SV40-specific proteins on the cell surface suggests that SV40 tumor-specific transplantation antigen may be an antigenic entity different from T- or U-antigen.

Adenoviruses, Human↗