Tuberculous liver abscess is uncommonly seen in our experience. We report a case of a 17-year-old boy who presented with typical clinical features of liver abscess, where a diagnosis of tuberculous liver abscess was made on laparotomy and biopsy of the abscess wall.

Adolescent↗

PubMed1986

Sandwich nucleic acid hybridization: a method with a universally usable labeled probe for various specific tests.

Nucleic acid hybridization is widely used for scientific applications but essentially restricted to specialized laboratories. The use of recombinant m 13 phages as hybridization probes (Hu and Messing (1980) Gene 17, 271; Messing (1983) Methods Enzymol. 101, 20) offers a considerable advantage over the commonly used recombinant plasmids as the preparation of the DNA probe is very simple and it can easily be labeled directly, e.g. with isotopes with long half-life like 125I (Commerford (1971) Biochemistry 10, 11 (1983); Gu et al. (1983) Cancer (China) 2, 129; Han and Harding (1983) Nucleic Acids Res. 11, 14) and used for hybridization. However, as the application of nucleic acid hybridization for diagnostic and epidemiological purposes becomes almost unavoidable, the logistic problems of keeping numerous individually labeled hybridization probes increase considerably and may reach prohibitory levels in less well-equipped laboratories. In a new sandwich technique, the first step involves hybridization with an unlabeled recombinant m 13 DNA carrying an insert of the desired specificity. In a second step a universally usable labeled probe directed against the m 13 part of the recombinant phage DNA is applied. This reduces considerably the problems of preparing and keeping multiple labeled probes in stock.

Bacteriophages↗

PubMed1985

Suppression of cell-mediated immunity following oral feeding of mice with palmyrah (Borassus flabellifer L) flour.

Inbred mice fed with a 40% mixture of palmyrah flour pellets showed a significantly reduced capacity to mount a delayed-type hypersensitivity (DTH) response to sheep red blood cells (SRBC). Maximal suppression of DTH was noted after 6 days of feeding but was detectable even after 2 days. Maximal suppression occurred when flour feed was maintained into the sensitization period (with SRBC). The suppressive effect was transferable to normal mice fed with pellets ad libitum by viable spleen cells from mice fed with the 40% flour pellet mixture for a period of 2 days. Further characterization of the cells which were able to transfer suppression showed them to be T cells, some of which possess the Ly-1 surface antigen. The cells appear to be negative for Ly-2 surface antigen. Histologically, no evidence of lesions was noted in the liver and other organs of recipient mice which received cells from flour-fed donors. We thus favour the interpretation that oral feeding of mice with palmyrah flour induced the generation of T suppressor cells which were able to suppress the DTH response to SRBC. The significance and implications of these findings are discussed.

Animals↗

PubMed1985

Ovarian pregnancy--a case report.

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Adult↗

PubMed1985

Declining post-mortems: a cause for concern.

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Autopsy↗

PubMed1984

New developments in nucleic acid hybridization.

Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.

DNA, Viral↗

PubMedDate not supplied

High prevalence of human skeletal muscle sarcocystosis in south-east Asia.

The prevalence of human skeletal muscle sarcocystosis in Malaysia was determined by serial examination of formalin-fixed, paraffin-embedded sections of tongue tissues obtained from consecutive, routine autopsies of subjects aged 12 years or more. Of 100 tongues examined, 21% were found to contain Sarcocystis; 66 cysts were found. The number of cysts per case varied from 1 to 13. In one case, 5 cysts were found in a single tissue section. The age range of positive cases was from 16 to 57 years (mean 37.7 years). Prevalence did not differ with regard to race, sex or occupation. The prevalence of human muscular sarcocystosis in our study was higher than that reported elsewhere. Preferential localization of Sarcocystis in tongue or head and neck and/or genuinely high prevalence in south-east Asia are possible explanations for this observation.

Adolescent↗

PubMedDate not supplied

Schistosomiasis in Malaysia.

Schistosomiasis was discovered in Malaysia in 1975 in an autopsy case. Since 1975 autopsies, surveys, and resurveys have been carried out to identify animal hosts, snail intermediate hosts, and reservoir hosts. Seroepidemiologic tests involving enzyme-linked immunosorbent and circumoval precipitin methods have been used to determine the true incidence and prevalence of this protean disease among the Orang Aslis (aborigines) in Malaysia. With the use of better epidemiologic and parasitologic tools, more cases of schistosomiasis are being reported.

Animals↗