PubMed Health⌕ Search

Biomedical subjects

R Petzoldt

Publications and source records attributed to R Petzoldt.

At least 37 records · Page 2Linked to original sources
PubMed1994

Effect of time of insemination relative to ovulation on fertility with liquid and frozen boar semen.

Precise data on fertility results following peri- and postovulatory insemination in spontaneously ovulating gilts is lacking. Using transcutaneous sonography every 4 h during estrus as a tool for diagnosis of ovulation, the effects of different time intervals of insemination relative to ovulation were investigated with liquid semen (Experiment 1, n=76 gilts) and frozen semen (Experiment 2, n=80 gilts). In Experiment 3 (n=24 gilts) the number of Day-28 embryos related to the various intervals between insemination and ovulation was determined after the use of liquid semen. Using liquid semen the fertilization rates based on Day-2 to Day-5 embryos and the number of accessory spermatozoa decreased significantly in gilts inseminated with 2 x 10(9) spermatozoa per dosage in intervals of more than 12 h before or more than 4 h after ovulation. In the time interval 4 to 0 h before ovulation, comparable fertilization rates were obtained using frozen semen (88.1%) and liquid semen (92.5%). Fertilization rates and numbers of accessory spermatozoa decreased significantly when gilts were inseminated with frozen semen more than 4 h before or 0 to 4 h after the detection of ovulation. The percentage of Day-28 embryos was significantly higher following preovulatory insemination compared to inseminations 0 to 4 h and 4 to 8 h after ovulation. It is concluded that the optimal time of insemination using liquid semen is 12 to 0 h before ovulation, and 4 to 0 h before ovulation using frozen semen. The results stress the importance of further research on sperm transport and ovulation stimulating mechanisms, as well as studies on the time of ovulation relative to estrus-weaning intervals and estrus duration.

Journal Article↗
PubMed1992

Glycation of serum albumin and its role in renal protein excretion and the development of diabetic nephropathy.

The present study was designed to investigate whether microalbuminuria at the onset of diabetic nephropathy might be partially due to the glycation of serum albumin. It is postulated elsewhere (Ghiggeri et al., Proc. Eur. Dial. Transplant. Assoc. 21 (1984) 633-636) that the glycation of serum albumin and the subsequent cationization may induce microalbuminuria. To investigate whether a relationship exists between the amount of glycated albumin in its cationized form and the development, and progression of diabetic nephropathy, the urinary excretion of glycated albumin was studied in diabetic patients. The diabetic patients (type I and II diabetes) were divided into groups according to their albumin excretion rates: group I diabetics had a normal albumin excretion (n = 30, x = 4.2 mg/12 h); group II diabetes displayed microalbuminuria (n = 17, x = 38.6 mg/12 h); group III diabetics displayed macroalbuminuria (n = 21, x = 582.5 mg/12 h). The fraction of glycated albumin in serum (Glyco Gel Test Kit) was 0.032 in group I, 0.042 in group II, and 0.038 in group III, all these values were significantly higher than the value for the controls (0.014%; n = 17, 2 alpha = 0.001) as measured with the Glyco Gel Test Kit. The concentration of glycated albumin in the urine of the controls and group I was below the detection limit. Urine in group II contained only a glycated albumin fraction of 0.0002 of total albumin, and the fraction for group III was 0.0008. Isoelectric focussing (IEF) and chromato-focussing revealed native albumin with an isoelectric point of 4.7-4.9, and anionic glycated albumin with a pI of 3.0-4.2.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Cost reduction in drug therapy of diabetes].

The costs of control an therapy of diabetes mellitus are very expensive. A cost reduction is to be expected after successful therapy. For 1024 insulin dependent type I-diabetics with stable insulin requirements the mean costs remained unchanged. 456 insulin dependent type II-diabetics who reduced weight showed a slight reduction of mean costs. The cost of drug therapy were significantly reduced for 535 overweight type II-diabetics under different therapeutic modalities, who reached a weight reduction. The inpatient treatment of diabetes influences the costs of drug therapy. A cost reduction is to be expected for overweight type II-diabetics, who can reach a weight reduction.

Cost Control↗
PubMed1986

22Na and 86Rb effluxes from bull spermatozoa.

Active transport of sodium and potassium has been postulated for bull sperms by various authors. In the present paper the sodium and rubidium efflux was determined by tracer kinetics. For uninhibited sodium and rubidium efflux a rate constant of 8.9 +/- 2.13/min and 13.9 +/- 3.89 min, respectively, was found for a bull sperm suspension (20 vol.%, 310 K). After ouabain treatment (0.1 mM), a reduction of the rate constant of sodium efflux to 5.1 +/- 1.06/min was found. After cryopreserving (pelletizing process) the majority of samples investigated did not exhibit any inhibition of sodium and rubidium efflux as compared with fresh bull sperms. The inhibition of sodium efflux observed in some cases corresponds to the reduction of the rate constant of sodium efflux caused by ouabain. At storage in seminal plasma (24 h, 278 K) the rate constant of sodium efflux is reduced to 2.7 +/- 0.25/min. Both after ouabain treatment and after cryopreserving of sperms having a reduced rate constant the motility of bull sperms is reduced. It is concluded from the results that in bull sperms there exists an ouabain-sensitive sodium efflux, the inhibition of which reduces the rate constant of sodium efflux by 42%. The ouabain-sensitive sodium efflux is related to the motility of the bull sperm cell.

Animals↗
PubMed1985

[Diabetes control under occupational conditions].

62 male diabetics worked in hospital workshops while their metabolic state was assessed. In each patient work activity, periods of work and rest and employment-related eating breaks were maintained. The metabolic state was regularly tested during physical activity simulating their actual employed work. In these circumstances there was a definite fall in blood sugar levels in the course of the day. Low values and hypoglycaemia were more frequent depending on the degree of physical activity, especially in the morning. The management of diabetes was adjusted to these metabolic requirements. Insulin dose was decreased and (or) carbohydrates were taken more frequently or in larger amounts. This adjustment in diabetes management was recommended to the diabetics for their normal everyday employment activity. Control of the diabetes was better on discharge from hospital and for some time afterwards.

Adolescent↗
PubMed1985

A rapid quantitative method based on motility of bull sperm cells for in vitro toxicity testing of biomaterials.

A quantitative method for in vitro toxicity testing of biomaterials, based on measurements of time-dependent changes of bull sperm motility, is described. In comparison to the haemolytic and toxic effects of biomaterials such as polyurethanes, poly(vinyl chloride) tubes and bioglass ceramics on erythrocytes and the proliferation rate of human embryonic lung fibroblasts, this method is shown to be more sensitive. The simplicity, rapidity and reproducibility of the test under application of genetically identical cells are advantages that make it suitable for screening large numbers of samples. The quantification of test results allows inter-laboratory comparison.

Animals↗
PubMed1982

[Palatinite in type II diabetics. Effect on blood-glucose, serum-insulin, C-peptide and free fatty acids].

In a randomized cross-over study, 24 type II diabetics were given at first 50 g palatinite and later 50 g glucose or vice versa in the morning before food intake. After administration of glucose there was a definite rise in blood-glucose, serum-insulin and C-peptide concentrations. After palatinite the rise of blood-glucose as well as serum-insulin and C-peptide was significantly less. Subjective side-effects were noted only after a single high dose of palatinite. Nonetheless, palatinite appears to be suitable as a sugar substitute in a diabetic diet, since in comparison with glucose there are no significant changes in blood-sugar levels and no additional insulin consumption is induced. Furthermore, because of its low energetic utilization it has an advantage over other sugar substitutes.

Adult↗