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Biomedical subjects

R Pineda

Publications and source records attributed to R Pineda.

31 records · Page 2Linked to original sources
PubMed1994

Expression of adhesion molecules in poststreptococcal glomerulonephritis.

Because adhesion properties of leukocytes are important for the influx and localization of leukocytes in sites of inflammation, we studied, the expression of intercellular adhesion molecule 1 (ICAM-1), lymphocyte-function-associated antigen 1 (LFA-1), vascular cell adhesion molecule- 1 (VCAM-1) and CD 11b in 14 kidney biopsies of PSGN patients, arbitrarily divided into early biopsies (less than 15 days after onset of PSGN) and late biopsies (17-90 days). In PSGN, intraglomerular ICAM-1 expression was increased in early biopsies (score 3.1 +/- SEM 0.2; P < 0.005) and decreased with time; in late biopsies the score (2.0 +/- 0.2) was similar to that of normal kidney (1.3 +/- 0.3). In the interstitium ICAM-1 was increased (early PSGN = 836 +/- 56 positive cells/mm2, late = 552 +/- 60.0; versus normal = 364 +/- 12.4; P < 0.05). LFA-1 expressing cells in glomeruli were also increased in early biopsies (10.0 +/- 2.1 positive cells per glomerular cross-section (gcs), versus normal 2.9 +/- 1.4; P < 0.05). In the interstitium, LFA-1 positive cells were increased (early PSGN = 221 +/- 79.6 cells/mm2, late PSGN = 134.5 +/- 45.1, normal = 21 +/- 8.7; P < 0.05). VCAM-1 in glomeruli and interstitium was not increased in PSGN. Our studies demonstrate increased expression of adhesion molecules ICAM-1 and LFA-1 in the kidney of PSGN patients, and these findings were more pronounced in early biopsies; adhesion molecules are probably involved in the inflammatory infiltration of this disease.

Adolescent↗
PubMed1993

Rapid karyotyping in ectopic pregnancies.

The aim of this study was to determine the feasibility of karyotyping ectopic Fallopian tube pregnancies utilizing dividing cytotrophoblast cells. Villi from 78 ectopic conceptuses were processed by the direct chromosome technique and cytogenetic diagnosis was successful in 60 cases (76.9 per cent). The amount of villi obtained, as well as villus morphology, was correlated with cytogenetic success rate. Histological examination of the Fallopian tube was also carried out. A total of 47 cases were chromosomally abnormal (78.3 per cent), which is the highest frequency of cytogenetic abnormalities reported to date.

Chorionic Villi Sampling↗
PubMed1993

Human retinoblastoma cells express alpha B-crystallin in vivo and in vitro.

alpha B-crystallin is a major lens protein that is a member of the heat-shock family of proteins. Using immunohistochemical and northern blot techniques, we now demonstrate its presence in freshly-fixed retinoblastoma tissue. The protein is also abundantly expressed in cultured human retinoblastoma cells (Y-79 NEI, WERI Rb-1) as well as two subcultured Y-79 lines (ATCC and GM01232C). High expression of alpha B-crystallin may be involved in tumor growth and/or be a marker for general oncogenic "stress" in the tumor tissue.

Animals↗
PubMed1992

'Black cornea' after long-term epinephrine use.

Fifteen years after a partial maxillectomy and radiation therapy for left antral carcinoma, a 53-year-old woman presented to the Eye Plastics and Orbit Service of the Massachusetts Eye and Ear Infirmary, Boston, with phthisis and a large, black corneal lesion in the left eye. She had been treated for unilateral glaucoma in the left eye for more than 10 years with topically administered epinephrine borate, timolol maleate, and pilocarpine hydrochloride. Clinically, the lesion was smooth, black, and homogeneous, and was thought to represent uveal prolapse covered by a thin layer of epithelium. An eyelid-sparing anterior exenteration was performed. Histopathologic examination revealed an acellular, homogeneous substance that stained positively with the Fontana Masson stain for melanin and bleached with potassium permanganate, findings consistent with corneal adrenochrome deposition. Since adrenochrome can be easily dissected free from the cornea, this case illustrates that misdiagnosing adrenochrome deposition may lead to unnecessary surgery.

Cornea↗
PubMed1991

Diuretics, hypochloremia, and outcome in bronchopulmonary dysplasia patients.

We evaluated retrospectively the outcome of patients with severe bronchopulmonary dysplasia who required hospitalization (greater than 90 days) to determine the role of diuretics aminophylline and chloride deficiency in the fatal outcome of these patients. Total cumulative amounts of furosemide, chlorothiazide, spironolactone accrued during hospitalization (dosage per patient per day), and a diuretic index (logarithm of the sum of the cumulative dosage of the three diuretics) were calculated for each patient. Other predetermined variables included in the analysis were birth weight, gestational age, duration of mechanical ventilation, duration of oxygen therapy, caloric intake, fluid and electrolyte intake, echocardiographic data, and duration and type of acid base balance disturbances. No differences were found between survivors and nonsurvivors in diuretic and aminophylline use. Hypochloremia did not discriminate between the groups. Duration of mechanical ventilation, the ratio between days with serum bicarbonate concentration greater than 30 mEq/l and total days of hospitalization, and the difference between head circumference at discharge or death and birth head circumferences were the most important factors which contribute to the difference between survivors and non survivors.

Acid-Base Imbalance↗
PubMed1990

Expanded spectrum of findings in Marden-Walker syndrome.

Recently, we examined a small-for-gestational age infant with blepharophimosis, congenital contractures of elbows, hips, and knees, fixed facial expression, and hypotonia. These congenital anomalies are consistent with a diagnosis of the Marden-Walker syndrome. The infant also had an omphalomesenteric duct, left hypoplastic kidney, hypoplastic right lower lobe of the lung, and displacement of the larynx to the right; these anomalies have not been described previously in this syndrome. A summary of the clinical manifestations of the previously reported patients is presented.

Abnormalities, Multiple↗
PubMed1990

[Prevalence of hepatitis B surface antigen in general population of coast, mountain and forest regions of Peru. A preliminary report].

A prospective study was performed in order to establish HBsAg prevalence on population living in urban areas of three geographical regions of Peru. There were included 100 apparently healthy people of each city, with a residence major to 5 years, no migrate neither transitory. Each subdivided group in 50 adults and 50 children. Of these last ones 15 between 1 to 7 years old and 20 cases between 7 to 14 years old. Samples were obtained by venipuncture, then they were centrifuged and the serum obtained was stored at -20 degrees C before being sent to Lima for processing. Determination of HBsAg was made by the Elisa's technic (Abbott Lab-III.-USA). In this preliminary report we informed about results in 7 cities (Lima, Iquitos, Chiclayo, Arequipa,Ica, Chachapoyas y Tarapoto) that include 680 persons of which 373 were adults and 307 children. The HBsAg, was positive in 26 cases (3.8%). In the adult group were 13 positives (3.4%), and in the children also 13 cases (4.2%). Result of all sample shows figures of prevalence (3.8%) major to that reported before at national level. The same phenomenon was observed in the cost while in the jungle our results were similar to those found before. In the andean area not studied previously there were obtained similar results to the rest of the country. It is also interesting to mention the high percentages of prevalence in children. These results indicate that hepatitis B is a serious problem of public health in our country that merit to take immediate prophylactic measures.

Adult↗
PubMed1989

Ocular changes in the mucopolysaccharidoses after bone marrow transplantation. A preliminary report.

Metabolic correction and physiologic response were evaluated after bone marrow transplantation in mucopolysaccharidosis. Eleven patients were prospectively evaluated to determine the effect of bone marrow transplantation on the progressive ocular manifestations of these disorders. Follow-up of 0.6 to 2.8 years after successful donor stem cell engraftment showed that some patients had slow clearing of the corneal clouding, reduction of intracytoplasmic inclusions in the conjunctiva, resolution of optic nerve edema, and stabilized or improved retinal function as determined by electroretinography. These preliminary results suggest that early bone marrow transplantation may alter some of the progressive ophthalmic characteristics of the mucopolysaccharidoses. Long-term follow-up is necessary to determine if these early alterations in the ocular features are predictive of a prolonged functional improvement in the visual status.

Bone Marrow Transplantation↗
PubMed1988

[Gastric adenocarcinoma surgically treated. A multivariate analysis of prognostic factors].

One hundred and twelve curatively resected gastric adenocarcinomas were studied retrospectively to appreciate the survival factors. Twenty different criteria (clinic, histologic and therapeutic parameters) were assessed using univariate and then multivariate analysis (semi parametric regression (COX's) model). Only three criteria were very important according to the multivariate analysis: 1) invasion of neighboring organs (p less than 0.006) with a relative risk score (RRS) of 4.26; 2) intravascular or intralymphatic tumor embols outside the tumor (p less than 0.004; RRS = 2.11); 3) invaded distal nodes (located at the origin of the vessels (p less than 0.04; RRS = 1.88). A prognosis index was described according to these results. A repartition of the patients in three prognostic groups according to these 3 criteria was proposed. Future, trials should consider these three different prognostic groups.

Adenocarcinoma↗
PubMedDate not supplied

Late onset of haze associated with viral keratoconjunctivitis following photorefractive keratectomy.

BACKGROUND: To report a newly recognized cause of late onset of corneal haze following photorefractive keratectomy (PRK). METHODS: A 35-year old woman who underwent uneventful PRK and subsequently developed viral keratoconjunctivitis 1 year after treatment. RESULTS: During resolution of the viral keratoconjunctivitis, the patient developed extensive central corneal subepithelial infiltrates and anterior stromal fibrosis in the PRK ablation zone. More than 1 year after the initial infection, the central subepithelial fibrosis persisted, complicated by myopic regression, central steep island on videokeratography, and loss of five lines of spectacle-corrected visual acuity. By the end of the second year after the infection, visual acuity had improved with disappearance of the central steep island on videokeratography. However, the myopic regression and subepithelial fibrosis remained. CONCLUSION: Patients who undergo PRK may be at greater risk of visual loss following viral keratoconjunctivitis secondary to persistent subepithelial infiltrates and/or stromal fibrosis within the PRK treatment zone.

Adenovirus Infections, Human↗