PubMed Health⌕ Search

Biomedical subjects

R Pokorný

Publications and source records attributed to R Pokorný.

11 recordsLinked to original sources
PubMed2006

Iodine saturation of Roma neonates in prague is not at an optimum level.

AIM: The purpose of our study was to determine urinary iodine as an indicator of iodine supplementation in Roma (Gypsy) neonates compared to majority population neonates. METHODS: The groups studied were formed by 30 full-term Roma neonates and 151 majority population neonates. Iodine was determined from samples of urine collected on the 4th day after delivery, after alkaline ashing, using the Sandell-Kolthoff method. RESULTS: The median of urinary iodine in Roma neonates was 92.15 microg/l urine and in neonates from majority population mothers it was 109.20 microg/l urine. The mean of urinary iodine in Roma neonates was 114.55 microg/l urine (SD 71.68 microg/l) and in neonates from majority population mothers it was 141.86 microg/l urine (SD 87.42 microg/l). The difference was not statistically significant. Majority population mothers more frequently consumed nutrition supplements containing iodine as well as fish. CONCLUSIONS: Compared to older data, supplementation of neonates with iodine is higher. However, it does not reach optimum levels. The urinary iodine median in Roma neonates lies in the mild iodine deficiency band.

Adult↗
PubMed2004

Chloride transport in the vegetative mycelia of filamentous fungus Trichoderma viride.

The influx of chlorides into Trichoderma viride vegetative submerged mycelium was measured by means of the radionuclide (36)Cl(-). It was found that the (36)Cl(-) influx was time-dependent (the steady-state was established with t(1/2 )= 25 min at 25 degrees C), pH-dependent (with pH optimum between 4-5.5), temperature-dependent (at about 15 degrees C), and concentration-dependent (K(M)(Cl(-))) = 47.6 +/- 4.2 micromol x l(-1); J(max) = 11.5 +/- 0.7 pmol(Cl(-)) x min(-1). mg(dry mass) (-1)). The (36)Cl(-) influx was inhibited by Br(-) but not F(-), I(-), SO(4)(2-), HPO(3)(2-) and HCO(3)(-). The presence of vanadate (P-type ATPase inhibitor) moderately stimulated the (36)Cl(-) influx but the presence valinomycin (electrogenic K(+) ionophore), salicylate (known to release Ca(2+) from Trichoderma viride internal stores) were without effect on the (36)Cl(-) influx. The results suggest that the (36)Cl(-) influx is mediated by a carrier and that the transport is electroneutral, probably Cl(-)/OH(-) antiport.

Biological Transport↗
PubMed2002

Copper complexes with bioactive ligands. Part II--Antifungal activity.

Antifungal activity of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of the composition Cu(2-MeSNic)2(MeNia)(2).4H2O (where MeNia is N-methylnicotinamide), and Cu(2-MeSNic)2(Nia)(2).2H2O (where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide, iNia, or ethyl nicotinate, EtNic) were tested on various strains of filamentous fungi by the macrodilution method. Most sensitive against copper(II) adducts with bioactive ligands were Rhizopus oryzae and Microsporum gypseum (IC50 1.5-2.3 mmol/L). The adducts with Nia, MeNia and EtNic at 5 mmol/L induced morphological changes in growing hyphae of Botrytis cinerea, mainly their intensive branching attached to release of cytoplasm with partial growth inhibition. Inhibition of sporulation (> 90%) of Alternaria alternata by Cu(2-MeSNic)2.H2O was observed as a change in the color of the colonies. The highest resistance was marked by B. cinerea and Fusarium moniliforme (average IC50 values 4.25 and 3.13 mmol/L, respectively). The presence of all bioactive ligands in copper(II) complexes caused an increase in the inhibition effect against model fungi (except significant inhibition activity of EtNic on R. oryzae).

Alternaria↗
PubMed2001

Copper complexes with bioactive ligands Part I--Antimicrobial activity.

Biological properties of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of composition Cu(2-MeSNic)2(MeNia)(2).4H2O (where MeNia is N-methyl-nicotinamide), Cu(2-MeSNic)2(Nia)(2).2H2O (where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide (iNia) or ethyl nicotinate (EtNic)) are reported. Gram(-)-bacteria (Escherichia coli) are more resistant against Cu(II) complexes than Gram(+)-bacteria (Staphylococcus aureus)--significant antistaphylococcal activity was found with Cu(2-MeSNic)2(MeNia)(2).4H2O (IC50 1.3 mmol/L). Candida parapsilosis was most inhibited by Cu(2-MeSNic)2.H2O and Cu(2-MeSNic)2(MeNia)(2).4H2O (IC50 1.4 mmol/L and 1.5 mmol/L, respectively). Biosynthesis of nucleic acids influenced by Cu(2-MeSNic)2(Nia)(2).2H2O indicated by incorporation of 14C-adenine (IC50(Ade) 0.31 mmol/L) is more sensitive than biosynthesis of proteins indicated by incorporation of 14C-leucine (IC50(Leu) 9.94 mmol/L). Cu(II) complexes with expressed antimicrobial activity showed no mutagenic activity.

Anti-Bacterial Agents↗
PubMed1999

Birth weight of neonates as an indicator of morbidity and mortality in adult age and factors which influence it.

In 28 neonates born by spontaneous delivery to healthy mothers with a normal course of gestation venous umbilical blood was examined to assess the immunoreactive insulin concentration. Moreover, the birth weight of the neonates was recorded, the weight increment of the mother during pregnancy and the body mass index at the end of gestation. By means of a questionnaire and the computer programme Progana the dietary intake of the mothers at the end of pregnancy was assessed and compared with recommended allowances. The assembled data were evaluated statistically by assessment of the correlation coefficient. The investigation revealed that the birth weight of the neonates in the group does not correlate with the immunoreactive insulin concentration in umbilical venous blood. Although the dietary intake of the mothers at the end of pregnancy is not ideal (fat and cholesterol intake predominates over carbohydrate and protein intake), it does not correlate with the birth weight of the neonates nor with the immunoreactive insulin concentration in umbilical venous blood. From these results the conclusion is drawn that neonates with a low birth weight do not have a higher plasma insulin concentration after delivery and that the mother's diet at the end of pregnancy does not influence the birth weight and insulin level in neonates.

Adult↗
PubMed1996

[Erythrocyte zinc-protoporphyrin and characteristics of iron- deficiency anemia in parturient women and neonates].

The authors examined the concentration of erythrocyte zinc-protoporphyrin (ZPP) by a haematofluorometric method in 175 women during delivery and the levels in their children. They assessed basic haematological indicators, the number of red cells, their volume, number of reticulocytes, haemoglobin concentration, haematocrit and sideraemia. All were average values within the physiological range in both examined groups. Statistical evaluation revealed a significant difference between mean values of sideraemia in mothers and children and mean values of the erythrocyte ZPP in both groups. The authors found also a medium significant correlation between ZPP and the erythrocyte volume and the erythrocyte volume and sideraemia. The correlation of different indicators was more marked after classification of the examined women and neonates into subgroups by haemoglobin concentrations and ZPP. In the subgroup of mothers with Hb values lower than 115 g/l a positive correlation was found with the number of reticulocytes red cell volume and sideraemia. In the subgroup of anaemic women (Hb concentration lower than 105 g/l) a negative correlation with the foetal sideraemia was found.

Adult↗
PubMed1992

Inter- and intra-specific differences in serum proteins of different species and subspecies of zebras.

1. Serum proteins of Equus grevyi, E. zebra hartmannae, E. burchelli boehmi, E. b. chapmanni and E. b. antiquorum were studied using starch-gel electrophoresis, 1-D polyacrylamide-gel electrophoresis, inhibitions of trypsin and chymotrypsin, immunoblotting, and specific staining for esterase. 2. Clear species-specific patterns were observed in albumin, transferrin, and for E. grevyi in protease inhibitor-1. Specific esterase was detected only in E. z. hartmannae. 3. Protein polymorphism was found in all studied species: E. grevyi--transferrin; E. z. hartmannae--protease inhibitor-1; E. b. boehmi--albumin, GC, transferrin, protease inhibitor-1, protease inhibitor-T; E. b. chapmanni--albumin, GC, transferrin, protease inhibitor-1; E. b. antiquorum--GC, transferrin, protease inhibitor-1. 4. Phenotype patterns of the polymorphic proteins were indicative of simple codominant inheritance. Further studies of polymorphism of protease inhibitor-2 and variability of protease inhibitor-X are needed. 5. alpha 1B glycoprotein in all zebra species was monomorphic. 6. The main transferrin components and alpha 1B glycoprotein of zebra (E. b. boehmi) were characterized for terminal sialic acid content.

Animals↗
PubMed1988

Evidence for the presence of alpha 1B-glycoprotein in mammalian sera: immunoblotting studies.

1. A monospecific antiserum to pig alpha 1B-glycoprotein (PO2) was produced in rabbits and was used to search for homologues of alpha 1B in sera of 41 mammalian species belonging to seven orders. 2. Specific reactions were detected in the sera of representatives of Insectivora, Primates, Carnivora, Proboscidea, Perissodactyla and Artiodactyla. No cross-reactions were observed in the sera of two species of Rodentia (mouse, rat). 3. Cross-reactions in the sera of Erinaceus europaeus, Homo sapiens and Macaca mulatta were rather weak; this indicates a greater structural difference between the alpha 1 B of Insectivora and Primates and that of the other mammalian orders. 4. Electrophoretic patterns of alpha 1 B were, in most cases, heterogeneous, the most heterogeneous being in ruminants. 5. Evidence was obtained that the alpha 1 B of sheep is identical with the earlier described (Juneja and Gahne (1980) Anim. Blood Grps Biochem. Genet. 11, 81-92.) polymorphic post-transferrin (Ptf).

Animals↗