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R Portocală

Publications and source records attributed to R Portocală.

30 records · Page 2Linked to original sources
PubMedDate not supplied

Interaction between ceruloplasmin and Sendai virus envelope components. Note ii. Some characteristics of Tween 20-solubilized envelope components isolated by gel-filtration.

Biological activities of Tween 20-solubilized Sendai virus envelope differ according to the structure of the components obtained, which depends on: conditions of virus disruption, presence or absence of detergent during gel-filtration, duration of maintenance, type of fragments contained in the samples, and can be correlated neither with the size of fragments, nor with protein concentration.

Adsorption↗
PubMedDate not supplied

Photodynamic effect of toluidine blue on MM virus.

The photodynamic effect of toluidine blue (TB) was studied on MM virus cultivated in L cells and on the RNA extracted form MM virus inoculated to mice by intracerebral route. MM virus infectivity is completely inhibited by the photodynamic action of TB, due to lesions at the molecular level of the viral RNA.

Encephalomyocarditis virus↗
PubMedDate not supplied

Immunoelectrophoretic characterization of Sendai virus antigens.

Antigens extracted from Sendai virus by solubilization with Triton X-100 (Tx). Tween-20 (Tw) or Triton X-100 and SDS (Tx-SDS) were analysed by rocket, crossed and tandem immunoelectrophoresis. The migration of these antigens through 1% agarose supplemented with guinea pig anti-viral envelope protein anti-serum shows that Tx and Tw antigens consist of three fractions differing in their quantitative ratios, while Tx-SDS exhibits five different fractions. The method proved to be useful for the identification of anti-host antibodies in immune antisera by means of an antigen extracted from the chorio-allantoic membrane of embryonated hen eggs.

Antigens, Viral↗
PubMedDate not supplied

Immunoelectrophoretic investigations on Sendai virus envelope antigens in relation to host antigens.

The different antigenic fractions characteristic of egg-cultivated Sendai virus envelopes were studied by immunoelectrophoresis. Virus-specific and host-specific fractions were identified. It was proved that incorporation of the host membrane fragments into the viral envelopes is not a passive phenomenon. During the last stages of its assembly and release, the virus incorporates into the envelopes some host glycoproteins, after an active biochemical processing resulting in a considerable reduction of their molecular weight, while the host antigenic determinants remain almost unaltered.

Animals↗
PubMedDate not supplied

Immunoelectrophoretic study of Sendae virus envelope antigens with hyperimmune sera prepared in different laboratory animals.

At similar HAI titers rabbit sera reveal a larger number of fractions (ten) in immunoelectrophoretic analysis of Sendai virus envelopes than do guinea pig sera, which only yield three distinct fractions. These differences are observed in both rocket and crossed immunoelectrophoresis, and are more marked in the latter. The number of fractions revealed does not differ in relation to the antigen used for hyperimmunization (intact Sendai virus, Triton-disrupted virus or envelope fractions extracted by Triton or Tween-ether). Solubilized Sendai virus envelopes obtained by Tween-ether or Triton treatment contain host antigens in their structure, the same as intact virus.

Animals↗
PubMedDate not supplied

Immunoelectrophoretic study of the host antigens of Sendai virus cultivated in different substrates (embryonated hen eggs and KB cells). Note I. Analysis of chorioallantoic membrane host antigens.

The immunoelectrophhoretic analysis of two preparations of the same virus cultivated in different substrates (Sendai/egg and Sendai/KB) demonstrate that virus envelopes contain: a) identical virus-specific antigens (but also some virus antigens with only partial immunochemical identity, or even non-identity); b) antigens incorporated by the virus from the host cell, during assembly and release. The latter differ according to the host, and some of them are only partially identical with the corresponding antigens of the host cell, which suggests that host cell glycoproteins are incorporated into the virus envelope after certain virus-induced modifications.

Animals↗
PubMedDate not supplied

Immunoelectrophoretic study on the host antigens of Sendai virus cultivated in different substrates (embryonated hen eggs and KB cells). Note II. Analysis of KB cell host antigens.

It could be demonstrated by immunoelectrophoretic analysis that the host antigen incorporated in the envelopes of Sendai virus cultivated in KB cells (Sendai/KB) is characteristic of these cells and does not occur in the envelopes of the same virus grown in the embryonated hen egg (Sendai/egg). This host antigen appears from the first passage in KB cells and is constantly maintained over subsequent passages. The glycoproteins released by Triton X-100 disruption from Sendai/KB envelopes have a concomitant antigenic specificity for both virus and host. In the case of Sendai/egg virus, exclusively virus-specific macromolecules are also released.

Antigens↗
PubMedDate not supplied

Study of host antigens in the envelopes of different myxo- and paramyxoviruses cultivated in the embryonated hen egg.

By immunoelectrophoretic analysis it was shown that different viruses--such as myxo- (influenza A0PR8) and paramyxoviruses (Sendai, SV5, NDV, mumps)--cultivated in the same host cells (chorioallantoic membranes) incorporate similar host antigens into their envelopes. The amount of host cell antigens incorporated into the virus envelopes differs from one virus to another. The fact that incorporation of host cell antigens occurs only after a virus-induced modification seems to be a general characteristic of enveloped viruses such as myxo- and paramyxoviruses.

Animals↗