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Biomedical subjects

R R Anderson

Publications and source records attributed to R R Anderson.

At least 19 recordsLinked to original sources
PubMed1995

Polarized light photography in the evaluation of photoaging.

BACKGROUND: The clinical characteristics of photodamaged skin, such as coarse and fine wrinkling, sallowness, hyperpigmentation, tactile roughness, laxity, and erythema, are not accurately evaluable from photographic records. OBJECTIVE: The purpose of this study was to develop accurate and reproducible photographic techniques that generate an evaluable record of the characteristics of photodamaged skin. METHODS: The method used involved illumination and photography through polarizing filters (polarized light photography). RESULTS: Polarized light photography generates images that selectively enhance either the surface features or the subsurface features of the skin, providing an accurate and evaluable record for evaluation of photodamaged skin. CONCLUSION: Polarized light photography, when coupled with precise framing and mapping, yields an accurate and evaluable record of photodamaged skin.

Humans↗
PubMed1995

Thermal relaxation of port-wine stain vessels probed in vivo: the need for 1-10-millisecond laser pulse treatment.

Although thermal relaxation times of cutaneous port-wine stain microvessels have been calculated and used to formulate laser selective photothermolysis, they have never been measured. A scheme to do so was devised by measuring the skin response to pairs of 585-nm dye laser pulses (250-360 microseconds each) as a function of the time interval between the two pulses, in five volunteers with port-wine stains. After a pump pulse delivering 80% of the fluence necessary for causing purpura, the fluence of a second probe pulse necessary to cause purpura was determined and was found to increase with the interval between the two pulses, in a manner consistent with thermal diffusion theory. Biopsy specimens were obtained from four of the five subjects to examine the nature and extent of vessel damage and to measure the port-wine stain vessel diameters. Using diffusion theory, the thermal relaxation time was calculated based on the measured vessel diameters. These calculated values are consistent with the increase in radiant exposure (fluence) of the probe pulse necessary to induce purpura for longer time delays. Two simple models for thermal relaxation of port-wine stain vessels are presented and compared with the data. The data and histologic assessment of the vessel injury strongly suggest that pulse durations for ideal laser treatment are in the 1-10-millisecond region and depend on vessel diameter. No dermatologic lasers presently used for port-wine stain treatment operate in this pulse width domain.

Adult↗
PubMed1995

In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast.

Confocal scanning laser microscopy of live human skin was performed to investigate the correlation of in vivo cellular and morphologic features to histology, the effect of wavelength on imaging, and the role of melanin as a contrast agent. We built a video-rate confocal scanning laser microscope for in vivo imaging of human skin. Using a 100 x microscope objective, we imaged high-contrast optical "sections" of normal skin, vitiliginous skin, and a compound nevus. In vivo "confocal histology" correlated well with conventional histology. The maximum imaging depth increased with wavelength: the epidermis was imaged with visible 400-700-nm wavelengths; the superficial papillary dermis and blood cells (erythrocytes and leukocytes) in the deeper capillaries were imaged with the near infrared 800-900-nm wavelengths. For confocal reflectance imaging, melanin provided strong contrast by increased backscattering of light such that the cytoplasm in heavily pigmented cells imaged brightly. In vivo confocal microscopy potentially offers dermatologists a diagnostic tool that is instant and entirely non-invasive compared to conventional histopathology.

Contrast Media↗
PubMed1995

Basic mechanism of in vitro pulsed-dye laser-induced vasodilation.

Vasodilation of rabbit carotid arteries induced by a pulsed-eye laser was studied in vitro to clarify the underlying mechanism. Artery segments were double cannulated in a pressure-perfusion apparatus which, under physiological conditions, allows for differential application of various solutions, pharmacological agents, and pulsed-dye laser light. Vasoconstriction was activated using both pharmacological and nonpharmacological agonists. Laser energy at a wavelength of either 480 or 575 nm was applied intraluminally in 1-microseconds pulses, which caused dilation of the arteries if hemoglobin was present in the lumen at sufficient concentration. Induced vasodilation did not specifically require the presence of hemoglobin; the same phenomenon could be repeated using an inert dye such as Evans blue as an optical absorber of laser energy. The optical density of the absorber, the number of applied laser pulses, and total amount of applied energy directly influenced the vasodilatory response. Laser-induced vasodilation was possible in both normal vessels and vessels denuded of endothelium. Pulsed-dye laser-induced vasodilation is therefore not a phenomenon mediated through chemical processes, but is rather a purely physical process initiated by the optical absorption of laser energy by the intraluminal medium, which probably induces cavitation bubble formation and collapse, resulting in the vasodilatory response of the vessel.

Animals↗
PubMed1995

Treatment of cafe au lait macules with lasers. A clinicopathologic correlation.

BACKGROUND: Cafe au lait macules (CALMs) respond variably to treatment with different lasers. This study was done to determine whether the type of laser and the individual histologic features of the CALMs could predict clinical response to treatment. Nine CALMs were treated with both the frequency-doubled Q-switched neodymium: YAG laser (wavelength, 532 nm; beam diameter, 2.0 mm) and the Q-switched ruby laser (wavelength, 694 nm; beam diameter, 5.0 mm). Both lasers were used at a fluence of 6.0 J/cm2. Biopsy specimens of the CALMs were obtained before and after treatment. Clinical follow-up was done at 1-, 3-, and 6-month intervals. OBSERVATIONS: Both lasers yielded variable responses to treatment. Two histologic subtypes of CALMs were identified, but these different subtypes did not predict clinical outcome after laser treatment. CONCLUSIONS: Cafe au lait macules respond variably to treatment with both the Q-switched ruby laser and the frequency-doubled Q-switched neodymium:YAG laser. Further research might address the effect of using multiple treatments. In view of these results, clinicians using lasers to treat CALMs should inform their patients of the potential for recurrence or darkening of CALMs.

Adult↗
PubMed1994

Thyroxine secretion rates during pregnancy in the rat.

Thyroxine secretion rates (TSR) at various stages of pregnancy in rats were measured by the radiothyroxine pool-turnover method. Groups of rats included normal controls (non-pregnant), days 5, 10, 20, and 22 of pregnancy and near term (within 24 hours prior to parturition if past 22 days of pregnancy). Each animal had blood samples taken just prior to injection of 10 microCi L-thyroxine (L-T4)-131I and at 12, 24, 36, and 48 hours afterwards. Determinations of thyroxine iodine (T4-I), volume of distribution (VD), fractional turnover rate (K), and L-T4 pool size were made from these samples. TSR was calculated as the product of (1.54) (T4-I) (VD) (K). An increase in TSR occurred near term, 3.65 micrograms L-T4/day compared to 1.23 micrograms L-T4/day in the controls, was primarily due to an increase in VD from 33.9 ml in the controls to 90.0 ml near term. A rapid increase in TSR to 3.65 micrograms L-T4/day just prior to term was postulated to be due to a stimulatory action of relaxin in the presence of estrogen upon L-T4 utilization. The mechanism of the increase in TSR in pregnant rats is probably due to the effect of several hormonal changes in maternal tissues throughout pregnancy and to adaptation of the pregnant animals to higher metabolic needs of the rapid developing fetuses.

Animals↗
PubMed1992

Dye-enhanced laser welding for skin closure.

The use of a laser to weld tissue in combination with a topical photosensitizing dye permits selective delivery of energy to the target tissue. A combination of indocyanine green (IG), absorption peak 780 nm, and the near-infrared (IR) alexandrite laser was studied with albino guinea pig skin. IG was shown to bind to the outer 25 microns of guinea pig dermis and appeared to be bound to collagen. The optical transmittance of full-thickness guinea pig skin in the near IR was 40% indicating that the alexandrite laser should provide adequate tissue penetration. Laser "welding" of skin in vivo was achieved at various concentrations of IG from 0.03 to 3 mg/cc using the alexandrite at 780 nm, 250-microseconds pulse duration, 8 Hz, and a 4-mm spot size. A spectrum of welds was obtained from 1- to 20-W/cm2 average irradiance. Weak welds occurred with no thermal damage obtained at lower irradiances: stronger welds with thermal damage confined to the weld site occurred at higher irradiances. At still higher irradiances, local vaporization occurred with failure to "weld." Thus, there was an optimal range of irradiances for "welding," which varied inversely with dye concentration. Histology confirmed the thermal damage results that were evident clinically. IG dye-enhanced laser welding is possible in skin and with further optimization may have practical application.

Animals↗
PubMed1992

Endometrial ablation by means of photodynamic therapy with photofrin II.

OBJECTIVE: Photodynamic therapy is a technique in which tissue is irradiated with light after the use of a photosensitizing drug that produces singlet oxygen, which has a cytotoxic effect. The feasibility of using photodynamic therapy with photofrin II for endometrial ablation was studied. STUDY DESIGN: Fifty-eight rabbits were studied. Preferential uptake of photofrin II by endometrial tissue, compared with the myometrium, was established by drug extraction and fluorescence microscopy after administration of photofrin II intravenously. Dosimetry for endometrial ablation was established by administering photofrin II in 1, 2, 5, and 10 mg/kg doses and laser light (630 nm) at radiant exposures of 100 and 200 J/cm2. Histologic examination was performed at 24 hours, 5 days, and 10 days after treatment. There were two control groups. One group received laser light but no photofrin II, and the other received photofrin II without laser light. RESULTS: The concentration of photofrin II was three times higher in the endometrium than in the myometrium at doses of 1 and 2 mg/kg. Fluorescence microscopy of frozen sections of endometrium and myometrium showed a predominantly perivascular fluorescence from photofrin II. A dose of 1 and 2 mg/kg and a flow of 100 J/cm2 was adequate for endometrial ablation in rabbits. At 24 hours after treatment there was extensive hemorrhage and evidence of cell death in the entire endometrium and mild hemorrhage in 10% to 50% of the inner circular layer of the myometrium. At 5 days after treatment necrosis of the entire endometrium and the inner half of the myometrium was seen, but the outer half of the myometrium and the serosa were normal. There were no cases of uterine perforation. Similar results were seen at 10 days after treatment, except for the additional presence of inflammatory cells. Neither control group (drug without light, light without drug) showed any injury to the endometrium at 24 hours. CONCLUSION: We conclude that endometrial ablation can be effectively achieved in rabbits by means of photodynamic therapy with photofrin II without significant complications.

Animals↗
PubMed1992

Determination of optical properties of turbid media using pulsed photothermal radiometry.

Pulsed photothermal radiometry (PPTR) measures blackbody radiation emitted by a sample after absorption of an optical pulse. Three techniques for obtaining the absorption coefficient of absorbing-only, semi-infinite samples are examined and shown to give comparable results. An analytic theory for the time dependence of the PPTR signal in semi-infinite scattering and absorbing media has been derived and tested in a series of controlled gel phantoms. This theory, based on the diffusion approximation of the radiative transport equation, is shown to model the time course of the detected signal accurately. Furthermore, when the incident fluence is known, the theory can be used in a non-linear, two-parameter fitting algorithm to determine the absorption and reduced scattering coefficients of a turbid sample with an accuracy of 10-15% for transport albedos ranging from 0.42-0.88.

Algorithms↗
PubMed1992

Changes in milk pH and bicarbonate during 20 days of lactation in the guinea pig.

Milk samples were obtained from lactating albino guinea pigs after 4 to 6 h of separation from their offspring. The samples were collected in 5-ml glass vials and capped immediately. They were transported rapidly to a pH-gas analyzer for measurements of pH, bicarbonate, base excess, and partial pressure of CO2. Eight guinea pigs were sampled daily for 20 d for a total of 160 observations. Analysis of variance indicated animal and day differences. Regression analyses over days revealed equations of best fit to be quadratic, although stepwise trends were observed in the data as well. The quadratic model for pH was Y(pH) = 6.996 - .057X + .002X2, where X was day of lactation (R2 = .54). The model for bicarbonate was Y (-HCO3 in milliequivalents per liter) = 8.896 - .659X + .0202X2 (R2 = .60). For base excess, the model was Y (in milliequivalents per liter) = -22.65 - 1.99X + .069X2 (R2 = .58). The model for partial pressure of CO2 was Y (millimeters of Hg) = 38.63 + 1.06X - .080X2 (R2 = .33). Decreases in pH and bicarbonate were parallel to changes in mammary epithelial cells of guinea pigs in which degeneration of mechanisms responsible for two important ingredients relating to milk volume occur. These have been identified previously as reductions in lactose and potassium. Synthesis of the enzyme carbonic anhydrase with its resultant bicarbonate production may be related in some way to these components of milk secretion.

Acid-Base Equilibrium↗
PubMed1992

Comparison of trace elements in milk of four species.

The objective of this study was to compare trace elements in milks of four species. Milk samples of 4 ml or more were obtained from guinea pigs, dairy cattle, horses, and humans. The milks were analyzed for the trace elements Al, B, Ba, Cu, Fe, Li, Mn, Mo, Si, Sr, Ti, and Zn by inductively coupled argon plasma spectroscopy. Zinc ranged from more than 4 ppm in guinea pig milk to less than 2 ppm in mare milk. Strontium was over 1 ppm in the milk of guinea pigs and less than .1 ppm for the human. Iron ranged from over .7 ppm for the guinea pig to less than .2 ppm for the cow. Copper was over .5 ppm in guinea pig milk and only .05 ppm for cow milk. Boron ranged from .59 to .10 ppm, Si from .58 to .16 ppm, Al from .45 to .10 ppm; and Ba from .22 to .08 ppm in milks of the four species studied. Titanium ranged from a trace to .11 ppm. Lithium, Mn, and Mo all were less than .04 ppm. Milk Mn was surprisingly low relative to bodily needs.

Animals↗
PubMed1992

Burn depth estimation using indocyanine green fluorescence.

Expedient primary excision of deep dermal and full-thickness burn wounds with subsequent skin grafting is the standard of care in most burn institutions, but differentiating full-thickness from partial-thickness burns is often difficult. Because accurate early assessment of burn depth may improve care, a variety of technical methods have attempted to measure burn depth but these methods have had limited success. We describe a new technique to determine burn depth that uses infrared (840- to 850-nm) fluorescence emission from intravenously administered indocyanine green following excitation with infrared (780 nm) and UV light (369 nm). Full-thickness and partial-thickness burns in hairless rat skin were distinguished based on the infrared-induced and UV-induced fluorescence intensity ratios relative to normal, unburned skin immediately after the burn and on post-burn days 1 through 3 and 7. Dual-wavelength excitation of indocyanine green infrared fluorescence can delineate full-thickness from partial-thickness burns at an early date, allowing prognosis, surgical planning, and early primary excision and grafting.

Animals↗
PubMed1991

A laser densitometer for selective spot analysis on dot blots and two-dimensional gel autoradiograms.

We describe an inexpensive densitometer, employing a small HeNe laser and an IBM-compatible personal computer that performs accurate measurements of selected spots on two-dimensional gel autoradiograms or chromatograms with an accuracy and a sensitivity equal or superior to those of many commercial instruments. Our open-table design allows the operator to visually monitor the scanning process in room lighting, and provides great flexibility in both the size and the nature of items to be scanned. The instrument has two moving parts (a prism and a small motor). A commercially available software package (ASYST) acquires digital data, graphs the data on the TV monitor, converts the data to optical density or to radioactive incorporation (cpm), subtracts background, integrates peak areas, and stores data on disk. The total time for these operations is 20-30 s per spot. The instrument has a dynamic range of 0.25 to 3.0 OD units and can measure a 10,000-fold range of 14C or 35S isotope concentrations on autoradiograms. The complete device can be assembled with a hobbyist's knowledge of electronics, moderate programming abilities (no machine language required), and a cost of less than $3000, not including the IBM PC.

Costs and Cost Analysis↗