Biomedical subjects

R R Glisson

Publications and source records attributed to R R Glisson.

At least 19 recordsLinked to original sources

PubMed1996

Cryopreserved anterior cruciate ligament allografts in a canine model.

This study was done to determine the clinical and biomechanical properties of cryopreserved anterior cruciate ligament allografts at 3, 6, 9, and 24 months after transplantation. A companion study of autografts was done to evaluate the effects of the surgical procedure and preservation on the tissue. The knee joints of 69 mongrel dogs (allograft n = 34, autograft n = 35) weighing 17 kg to 25 kg were grafted. Biomechanical results showed that maximum load was less in allografts than in autografts at each time interval. Deformation and slope were not significantly different between allograft and autograft. An apparent delay in revascularization and cellular repopulation was found in allografts compared with autografts, and collagen type I/type III ratios were similar in both grafts. DNA analysis indicated complete replacement of DNA in the graft by the host. Allografts provide adequate functional stability for daily activities in the canine model up to 2 years after transplantation. Biomechanical properties were relatively poor in allografts, which may lead to failure under more strenuous activity. The biologic similarity to autografts may indicate future remodeling potential.

Animals↗

PubMed1995

Augmentation of meniscal repairs with cyanoacrylate glue.

Cyanoacrylate glues are biodegradable, bacteriostatic, hemostatic adhesives which have been used experimentally in surgical procedures on many tissues. This study evaluates the efficacy of cyanoacrylate glues used for in vitro bovine meniscus repair. Our hypothesis was that a local adhesive might improve fixation of meniscus tears reapproximated with suture, or obviate the need for suture. Three high-viscosity flexible cyanoacrylate glues were tested and their tensile strengths compared to those of suture alone and suture augmented with glue. The flexible formulation enables the glue to deform with the meniscus during movement of the knee joint. Bovine meniscus tissue was used as the model to compare the tensile strength at the meniscus repair site using the 300 cps glue in combination with 3-0 prolene suture, 300 cps cyanoacrylate glue alone, and suture alone. Twenty specimens were included in each repair group, and the peak load to failure was normalized for surface area and subjected to analysis of variance. Results of the analysis showed that the peak load to failure of the combination of cyanoacrylate glue plus suture was significantly higher than that of glue alone (P < .0001), but not significantly higher than that of suture alone (P = .18). However, in tissues repaired with suture, tissue separation at the tear site was noted almost immediately after distraction started, as a result of the elasticity of the suture. The combination of cyanoacrylate glue plus suture provided the maximum tensile strength before tissue separation. Because tissue separation may interfere with the meniscal healing process, glue used in combination with suture appears to offer some advantage mechanically.(ABSTRACT TRUNCATED AT 250 WORDS)

Adhesiveness↗

PubMed1995

An electromyographic study of arm muscles during climbing.

Upper extremity muscle injuries from rock climbing are common. Knowledge of the activity of specific muscles during climbing may allow the development of training programs to reduce these injuries. This study evaluated the electrical activity of the first interosseous (IN), brachioradialis (BR), flexor digitorum superficialis (FD), and biceps brachii (BB) muscles in seven climbers by integrated electromyography (IEMG) during finger-tip pull-ups. The climbers, with forearms pronated, performed three consecutive pull-ups. Each pull-up consisted of: (1) hanging using four fingers of each hand, (2) pull-up to maximum elbow flexion, (3) slow return to starting position. The IEMG during maximum voluntary contraction (MVC) was obtained for each muscle separately, and the IEMG was normalized to MVC. During hanging, FD showed the highest normalized IEMG (0.64 +/- 0.20). During pull-up, the highest IEMG was produced by FD (0.69 +/- 0.25) and BR (0.67 +/- 0.19), while BB showed only 0.33 +/- 0.12 and IN 0.09 +/- 0.06. During lowering, FD again had the highest IEMG (0.74 +/- 0.24), while the EMG from BR was decreased to 0.42 +/- 0.14 and BB to 0.15 +/- 0.15. BR and BB showed an abrupt peak in EMG during pull-up and lowering, as opposed to FD which remained constantly highly activated, which suggests that FD does not contribute to elbow flexion even though it crosses the elbow joint. The high activation of FD and BR may explain their elevated incidence of injury during climbing. Thus, a reduction in climbing-related muscle injuries may be achieved by a training program that emphasizes conditioning of the BR and FD muscles.

Adult↗

PubMed1994

A biomechanical comparative analysis of two techniques for tibiotalar arthrodesis.

Two commonly used techniques for tibiotalar fusion were quantitatively compared using instrumented testing of the strength of the construct. The tibiae and tali from 10 pairs of fresh-frozen cadaveric limbs were used. One joint of each pair was fused using two 6.5-mm crossed cancellous screws from proximal to distal while the contralateral joint was fused using two 6.5-mm parallel cancellous screws from distal to proximal. Each specimen was subjected to cantilever bending and torsional testing by servohydraulic actuators. The bending tests included plantarflexion, dorsiflexion, inversion, and eversion, and measured the load during deflection applied 10 cm distal to the fusion site. The rigidity was expressed as newtons per millimeter of deflection. The torsional tests measured construct stiffness in external and internal rotation, and were expressed as newton-meters per degree of rotation. For the bending tests, the crossed screw construct was more rigid in eversion (23.1 N/mm, P = .0004) and dorsiflexion (16.9 N/mm, P = .02), while the parallel screw construct was more rigid in inversion (22.8 N/mm, P = .02) and plantarflexion (22.3 N/mm, P = .0007). In torsional testing, the crossed screw construct was at least 1.5 times stiffer than the parallel screw construct in resisting internal (1.7 N-m/deg versus 0.9 N-m/deg, P = .0001) and external (1.4 N-m/deg versus 0.9 N-m/deg, P = .02) rotation. In laboratory testing, the crossed screw technique is more rigid than the parallel screws, especially in resisting torsional stresses.(ABSTRACT TRUNCATED AT 250 WORDS)

Ankle Joint↗

PubMed1993

Graft isometricity in unitunnel anterior cruciate ligament reconstruction: analysis of influential factors using a radiographic model.

A radiographic model was developed to investigate the influence of three surgical variables on the change in attachment point distance (CAPD) of a hypothetical graft using the unitunnel technique of anterior cruciate ligament (ACL) reconstruction. Using three different femoral target points, we tested the hypothesis that varying the angle of knee flexion between 70 degrees and 110 degrees and varying the tibial starting point over a 4-cm range do not result in a significant variation in CAPD. We also tested the hypothesis that the CAPD from 0 degrees to 135 degrees is greater than the CAPD from 0 degrees to 90 degrees. There was a statistically significant correlation (r = 0.8465, P < 0.0001) between radiographically estimated and isometer-measured values of CAPD. The tibial starting point and the femoral target point were found to affect the CAPD significantly (P < 0.005). A more proximal tibial starting point was associated with a lower CAPD. Both the center of the anatomic femoral attachment of the ACL, and a point 1 mm medial to the junction of the roof and lateral wall of the femoral intercondylar notch and 6 mm anterior to its posterior margin, were associated with lower CAPD values than a target point 5 mm superior and posterior to the center of the femoral ACL attachment. The angle of knee flexion did not significantly affect the CAPD. The CAPD [0 degrees-135 degrees] was significantly greater than the CAPD [0 degree-90 degrees] for all combinations of variables (P < 0.0005).(ABSTRACT TRUNCATED AT 250 WORDS)

Anterior Cruciate Ligament↗

PubMed1992

The functional recovery of peripheral nerves following defined acute crush injuries.

This study evaluates the effect of crushing load on functional recovery of the sciatic nerve. Male Sprague-Dawley rats were divided into five groups: sham operation, resected sciatic nerve, and 100 g (13 mm Hg/mm2), 500 g (50 mm Hg/mm2), and 15,000 g (1,000 mm Hg/mm2) of sciatic crush load (groups 1-5). In groups 3-5, a 5-mm segment of sciatic nerve was crushed for 10 min using a specially designed crushing device. Motor functional recovery was assessed from hind-limb walking tracks by calculating a sciatic functional index. There was no detectable functional deficit in the group receiving sham operations, while the resected sciatic nerve group exhibited complete dysfunction for the full duration of the experiment. All groups subjected to crush exhibited an initial deficit that gradually recovered to normal by day 14 (100-g crush), day 39 (500-g crush), and day 53 (15,000-g crush). Histological changes were also related to the initial crushing load and the length of the recovery period. Results indicate that the crushing device described is able to administer an adjustable, defined crush injury to the rat sciatic nerve, and that the functional deficit resulting from such an injury can be easily monitored with a sciatic functional index. The rate of recovery of crushed nerves was directly related to the initial load. All crushed nerves recovered in this experiment, even after the application of a 15,000-g load for 10 min.

Animals↗

PubMed1992

The effect of extracorporeal shock wave lithotripsy on the prosthesis interface in cementless arthroplasty. Evaluation in a rabbit model.

The effect of extracorporeal shock wave lithotripsy on interfacial strength between prosthesis and bone in cementless arthroplasty was examined using a rabbit model. Paired femora, each implanted with fiber mesh porous coated titanium implants, were harvested from rabbits 15 weeks after implantation. In group I, one femur from each pair was exposed to lithotripsy treatment consisting of 2,000 shocks at 20 kV. In group II, one femur from each pair was exposed to 2,000 shocks at 26 kV. Contralateral femora from each pair served as controls in both groups. Mechanical pushout tests were conducted on the implants using a 1321 Instron testing machine at a constant rate of 1 mm/minute. Shock waves generated at 20 kV were found to have no significant decrease on either the prosthesis/bone interfacial strength or energy to failure of cementless implants. Shock waves generated at 26 kV produced a mean 17.45% decrease in the prosthesis/bone interfacial strength, which approached statistical significance (P = .062), and a 7.84% mean decrease in the energy to failure (P = .268). However, in four of the seven group II specimens, cortical fractures occurred. These findings suggest that lithotripsy will not aid in the removal of uncemented porous coated devices and lithotripsy inadvertently focused at an uncemented device will not disrupt significantly the prosthesis-bone interface.

Animals↗

PubMed1992

The effect of femoral stem geometry on interface motion in uncemented porous-coated total hip prostheses. Comparison of straight-stem and curved-stem designs.

We compared the magnitudes of motion between the prosthesis and bone during axial and torsional loading in seven matched pairs of fresh-frozen femora of cadavera in which an uncemented, collarless, isthmus-filling, straight-stem (Harris-Galante) prosthesis had been placed in one femur and an uncemented, collarless, proximal-filling, curved-stem (anatomic) prosthesis had been placed in the other femur. The comparison was performed in order to determine the effect of the geometry of the stem on the magnitude of motion. Single-limb-stance loads and combined axial and torsional loads were applied to the implanted femoral prostheses with the use of a jig that simulated acetabular and trochanteric loading. Extensometers were used to measure motion at the prosthesis-bone interface. The prostheses were then removed and were reinserted, with cement applied to the proximal porous coating to simulate ingrowth of bone. The single-limb-stance and combined axial and torsional loads were reapplied and the magnitude of motion was recorded again. No significant differences in the magnitudes of the motion were found between the femora in which the straight stem had been implanted and the femora in which the curved stem had been implanted, during either simulated single-limb-stance or low-intensity torsional loading. When large torsional moments (twenty-two newton-meters) were applied, significantly less motion occurred at the bone-prosthesis interface, both proximally (p = 0.019) and distally (p = 0.0013), in the femora with the curved-stem implant than in the femora with the straight-stem implant. When cement had been applied proximally, proximal and distal motion between the prosthesis and the femur was decreased during simulated single-limb-stance and during torsional loading in the femora with the straight stem and the femora with the curved stem.

Adolescent↗

PubMed1991

Morphologic and morphometric analyses of rat femoral arteries after crush injury.

Replantation after crush amputation has a relatively low success rate. An arterial crush injury was produced in rats under a range of pressure (8.5 to 2,551 g/mm2) and durations (10 to 60 minutes). The degrees of vessel injury and thrombus formation were evaluated as functions of both crush force and duration, and the healing process was followed for 8 weeks. Crushing resulted in morphologic damage to the arterial wall but did not impair patency. Damage was directly proportional to crush pressure and duration. Intimal denudation caused flat platelet adhesion but no thrombus formation or thrombosis. Re-endothelialization was completed by 2 weeks. Intimal hyperplasia appeared at 1 week and consisted of endothelia in the most luminal layers and smooth muscle in the deeper layers. the internal elastic lamina was intact in all specimens and was thought to minimize platelet aggregation on the arterial wall after denudation of the intima.

Animals↗

PubMed1991

Prosthetic silicone scaphoid strains: effects of intercarpal fusions.

To assess stress shielding by partial intercarpal fusion, strains within a silicon scaphoid prosthesis were measured in five fresh upper extremities while the wrist was moved through a functional range of motion before and after capitate-lunate and capitate-hamate-lunate-triquetral intercarpal fusions. Capitate-lunate fusions resulted in an overall 18.4% reduction in compressive strains and a 10.8% reduction in tensile strains. Capitate-hamate-lunate-triquetral fusions reduced compressive strains by 28.5% and tensile strains by 26.3%. Radially deviated positions and positions of extension produced no reduction in strains with the partial fusions. Modest strain shielding can be obtained with partial intercarpal fusions. The effect, however, is position dependent, and whether this degree of strain shielding can reduce the incidence of particulate synovitis remains unknown.

Arthrodesis↗

PubMed1991

Screw-augmented fixation of acetabular components. A mechanical model to determine optimal screw placement.

Sixteen embalmed hemipelves were used to determine the optimal acetabular screw placement to provide maximal screw pull-out strength in unicortical and bicortical screw fixation. The anterior column, superior ilium, posterior column, ischium, and pubis regions of the pelvis were tested using 6.5-mm titanium alloy screws and a hydraulic servo-controlled 1321 Instron testing machine. Force vs displacement data were acquired. Bicortical fixation was stronger than unicortical fixation in the four zones compared. This difference was significant in the superior ilium, posterior column, and ischium. The anterior column could not accept unicortical screws due to inadequate bone depth, which ranged between only 6 mm and 10 mm. Bicortical fixation was significantly greater in the superior ilium, posterior column, and ischium than in the anterior column or pubis. Unicortical fixation was greatest in the superior ilium. This information may aid decisions concerning the positioning of screws to augment acetabular component fixation.

Acetabulum↗

PubMed1990

Kinematics of the ulnar carpus related to the volar intercalated segment instability pattern.

Anatomic dissections were done on 18 fresh and 4 preserved cadaver upper extremities to study the pathology of the carpal kinetics and to define the ligaments involved in the volar intercalated segment instability pattern. In a normal wrist, the scaphoid, lunate, and triquetrum all rotate so that their distal surfaces tilt palmarward with radial deviation. With ulnar deviation, all three carpal bones rotate dorsally. In the volar intercalated segment instability pattern, the lunate remains tilted volarly instead of rotating dorsally with ulnar deviation. In this study changes in carpal motion were observed after serially sectioning the ligaments supporting the ulnar carpus before and after loads were applied to the wrist to simulate the dynamic conditions of the wrist. The ligaments were then repaired to help identify which repairs restored normal carpal motion with radial and ulnar deviation with and without axial loads. In these anatomic studies the volar intercalated segment instability pattern occurred when there was instability between both hamate and triquetrum and between the lunate and triquetrum. The main ligaments involved in this instability appeared to be the ulnar half of the volar arcuate ligament and the luno-triquetral ligament as division of these ligaments, particularly under axial loads, produced the most significant change in lunate rotation (p less than 0.05). Similarly repair of these two ligaments produced the most significant correction of lunate position particularly with maintenance of dorsal rotation of the lunate during ulnar deviation under axial loads (p less than 0.01).

Humans↗

PubMed1989

Effect of hyaluronic acid/chondroitin sulfate on healing of full-thickness tendon lacerations in rabbits.

Viscoat, a high-molecular-weight, highly purified hyaluronic acid (HA) and chondroitin sulfate (CS) compound, was instilled around rabbit plantaris tendon following full-thickness laceration and surgical repair. After 3 weeks of immobilization, no significant difference in adhesion strength or tensile strength of the healing tendons existed between Viscoat-treated tendons and controls. This contradicts previous studies which suggest that hyaluronic acid reduces postoperative tendon adhesions. Further studies examining tendon adhesions after less severe degrees of tendon injury and using direct, quantitative measurement techniques are warranted to demonstrate whether HA has a beneficial effect on tendon healing.

Animals↗

PubMed1988

Immediate and delayed neurorrhaphy in a rabbit model: a functional, histologic, and biochemical comparison.

The effect of timing of neurorrhaphy on neuromuscular function was studied. The extensor digitorum longus neuromuscular units of 51 rabbits were used, with repairs performed immediately, 3 weeks, 3 months, and 6 months after nerve transection. Neuromuscular function was assessed 3 months after nerve repair, as was histology, histochemistry, and muscle hydroxyproline content. Force generated by immediately repaired units was 93% of unoperated controls. All functional delayed repairs produced approximately 50% of control force. Mild endomysial and perimysial fibrosis was present in the immediate neurorrhaphy group. Fibrosis was mild to moderate in the functional delayed repairs and moderate to severe in the nonfunctional repairs. Total and regional hydroxyproline content correlated to both function and timing of nerve repair. The data demonstrate that immediate repair of peripheral nerve lacerations gives the best functional recovery in this rabbit model. If immediate repairs are not performed in the rabbit, approximately 50% to 75% of normal function can be obtained when repairs are delayed for periods of 3 weeks to 6 months.

Animals↗

PubMed1988

Partial excision of the triangular fibrocartilage complex.

A cadaver model was used to evaluate the effect of partial excision of the triangular fibrocartilage complex (TFCC) on ulnar wrist kinetics. Excision of less than two thirds of the horizontal portion of the TFCC was found to have no statistical effect experimentally on forearm axial load transmission. This data supports, from a biomechanical standpoint, partial excision of the central portion of the TFCC--a procedure advocated clinically by some for treatment of a central TFCC perforation.

Biomechanical Phenomena↗

PubMed1987

Forearm force transmission after surgical treatment of distal radioulnar joint disorders.

To evaluate the effect of silicone replacement of the distal ulnar, hemiresection arthroplasty and the Darrach procedure on ulnar support of the carpus, changes in force transmission to the radius and ulnar were measured using load cells in axially loaded cadaver arms. Testing of the intact specimens in neutral position showed that an average of 17% of the axial load was borne by the ulna whereas the load decreased to 3.6% after silicone arthroplasty, to 2.4% after hemiresection arthroplasty, and to 1.0% after the Darrach procedure. Only by placing the silicone cap on the ulna to lengthen it by 4 mm did the ulna load become within 75% of that in the intact specimen. In the intact specimen, ulna loading increased with wrist extension and ulna deviation and decreased with flexion and radial deviation, while after the surgical procedures there was no significant change in ulna loading with wrist position.

Biomechanical Phenomena↗

PubMed1986

A biomechanical comparison of the methods for treating Kienböck's disease.

Reduction of lunate compression is thought to promote revascularization of the lunate in patients with Kienböck's disease. The decompressing abilities of ulnar lengthening, radial shortening, capitate-hamate fusion, and scaphoid-trapezium-trapezoid (STT) fusion were examined in axially loaded, whole arm specimens. Lunate strain was monitored by electronic strain gauges and found to be proportional to the axial load borne by the bone. The STT fusions and the procedures to alter relative radial and ulnar length were successful in relieving lunate loading throughout a functional range of wrist motion and forearm rotation, but the capitate-hamate fusion was ineffective. Only the STT fusion resulted in a significant decrease in wrist range of motion. Incremental ulnar lengthening and radial shortening revealed that approximately 2 mm of length change maximizes lunate decompression without greatly increasing the risk of disorders of the distal radioulnar joint and ulnocarpal impingement.

Biomechanical Phenomena↗

PubMed1986

Force transmission through the distal radioulnar carpal joint: effect of ulnar lengthening and shortening.

Although numerous surgical procedures are performed on the wrist joint, little has been written regarding the effect of these procedures on the biomechanics of the wrist. This study has been undertaken to examine the in vitro consequences of ulnar lengthening and shortening. We have measured the axial forces transmitted through the distal radius and ulna and the pressure distribution on the articulating surface of the radius and triangular fibrocartilage complex for the intact wrist, with ulnar lengthening and with ulnar shortening. Lengthening of the ulna by 2.5 mm in the intact wrist increased the force borne by the ulna from 18.4% to 41.9% of the total axial load. Shortening of the ulna by 2.5 mm decreased axial load borne by the ulna to 4.3%. Removal of the articular disc portion of the triangular fibrocartilage complex decreased the load on the intact ulna from 18.4% to 6.2%. The peak pressure at the ulnolunate articulation increased from 1.4 N/mm2 for the unaltered wrist to 3.3 N/mm2 when the ulna was lengthened by 2.5 mm. These results suggest that the biomechanics of the wrist joint can be dramatically altered with relatively small (2.5 mm) changes in ulnar length and by removal of the articular disc portion of the triangular fibrocartilage complex.

Biomechanical Phenomena↗