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Biomedical subjects

R R Pascal

Publications and source records attributed to R R Pascal.

At least 55 records · Page 3Linked to original sources
PubMed1976

Electron microscopy and immunofluorescence of glomerular immune complex deposits in cancer patients.

Kidneys of 29 patients without clinical renal disease were studied by electron microscopy for the presence of glomerular basement membrane deposits: those from 11 of 20 patients with cancers of various sites, but only 1 of 9 patients without cancer contained electron-dense subendothelial deposits. The majority of these kidneys gave positive immunofluorescent reactions for immunoglobulin and complement. However, among a number of the cases studied, a lack of correlation between electron microscopic and immunofluorescence findings has yet to be investigated. Although the number of patients in this study is small because of the difficulty in obtaining tissue for electron microscopy, it is postulated that the deposition of immune complexes in the kidney occurs with a high frequency among cancer patients. The kidneys may thus be a valuable source for isolating tumor-associated antigens and corresponding antibodies.

Adolescent↗
PubMed1975

Adenocarcinoma of the small intestine arising in Crohn's disease. Demonstration of a tumor-associated antigen in invasive and intraepithelial components.

A segment of small intestine surgically removed from a man with intestinal obstruction was found to have coexisting regional enteritis and an invasive adenocarcinoma associated with an area of intraepithelial neoplasia. The cells of the adenocarcinoma and the intraepithelial neoplasia contained a tumor-associated surface antigen capable of reacting with an antiserum prepared against a colonic carcinoma. The importance of thorough sampling of specimens of regional enteritis in accurate reporting of the incidence of carcinoma is stressed, as is the use of immunohistologic techniques as an adjunct to the morphologic diagnosis of preinvasive neoplasia.

Adenocarcinoma↗
PubMed1975

Invasive and metastasizing carcinoma in a small adenomatous polyp of the colon: report of a case with demonstration of a tumor associated antigen.

An unusual case of metastasis to two lymph nodes from an adenocarcinoma arising in an adenomatous polyp of the sigmoid colon is presented. Invasion of the stalk could not be detected, but penetration of the muscularis mucosae was observed. Immunoperoxidase staining was used to detect an antigen in the invasive and metastatic components of the tumor that reacted with an antiserum to a tumor associated antigen. A focus of intraepithelial carcinoma was also detected by this method. The relevance of these findings to the adenoma-carcinoma sequence is discussed.

Adenocarcinoma↗
PubMed1975

Glomerular immune complex deposits associated with mouse mammary tumor.

Adult Paris R-III mice with localized mamary tumors were found to have subendothelial glomerular immune complex deposits by electron microscopy and immunofluorescence. In addition to IgG adn B-1-C, the glomeruli contained antigenic material that reacted with an antiserum to mouse mamary tumor virus. These findings support the hypothesis that many animals with congenitally or neonatally induced virus-related tumors are not immunologically tolerant to the oncogenic virus.

Animals↗
PubMedDate not supplied

Gut-trophic effects of keratinocyte growth factor in rat small intestine and colon during enteral refeeding.

BACKGROUND: Keratinocyte growth factor (KGF) induces proliferation of gut epithelium in rat models, but KGF-nutrient interactions have not been studied. An experimental model of fasting-induced gut atrophy followed by different levels of enteral refeeding was used to investigate the influence of nutrient availability on the gut-trophic effects of exogenous KGF. METHODS: After a 3-day fast, rats were enterally refed either ad libitum or at 25% of ad libitum intake for 3 subsequent days. Either intraperitoneal KGF (5 mg/kg/d) or saline was given in each dietary regimen. Wet weight, DNA, and protein content were measured as indices of full-thickness cellularity in duodenum, jejunum, ileum, and colon. Villus height in small bowel segments and crypt depth in all gut tissues were measured as specific indices of mucosal growth. RESULTS: Refeeding at 25% of ad libitum intake significantly decreased full-thickness cellularity and mucosal growth indices in duodenum, jejunum, and ileum. In the colon, only protein content fell significantly and crypt depth was maintained. KGF administration during 25% refeeding did not alter full-thickness indices in any small bowel segment or affect jejunal mucosal growth. In contrast, KGF normalized duodenal villus height (p < .01) and duodenal and ileal crypt depth (p < .05) only in the 25%-refed model. KGF significantly increased ileal villus height in both ad libitum and 25%-refed rats (by 43% and 48%, respectively, p < .05) and markedly increased colonic cellularity and mucosal crypt depth with both levels of refeeding (p < .01). CONCLUSIONS: Rat small bowel growth is more sensitive than colon to the level of enteral refeeding after a 3-day fast. KGF administration does not affect jejunal growth, but specifically prevents atrophy of duodenal and ileal mucosa during hypocaloric, hyponitrogenous refeeding. In ileum and colon, some KGF-mediated growth responses are independent of the level of enteral refeeding. Thus gut-trophic effects of KGF and KGF interactions with the level of nutrient intake are tissue-specific.

Animal Nutritional Physiological Phenomena↗