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Biomedical subjects

R Reverberi

Publications and source records attributed to R Reverberi.

16 recordsLinked to original sources
PubMed1992

Increased uptake of radio labelled white blood cells into abdomen of uremics on peritoneal dialysis.

Peritonitis and exit-site tunnel infection are frequent causes of CAPD drop out. We studied 9 patients, 8 treated by CAPD and 1 by IPD. These patients underwent sonographic and scintigraphic study of the abdomen. All scintigraphic examinations showed a visceral uptake. In two cases, sub-clinical bowel inflammation, demonstrated by scintigraphic study, preceded a gram negative peritonitis. The scintigraphic study with radiolabelled white blood cells may be useful in identifying chronic aseptic inflammations and some bowel and exit-site conditions which are possible risk factors in some cases of peritonitis.

Abdomen↗
PubMed1990

Clinical efficacy of five filters specific for leukocyte removal.

The clinical efficacy of 5 filters specific for leukocyte depletion was evaluated retrospectively. The patient population was composed of 191 thalassemics who received about 15,000 units of filtered blood in 28 months. The frequencies of reactions to filtered blood varied significantly (p less than 0.0001) according to the type of filter and were as follows: Erypur, 0.7%; Imugard, 2.7%; Leucostop, 0.7%; Miropore, 2.1%; Sepacell, 0.6%. There was a notable concordance between the efficacy in the leukocyte removal and the clinical performance of the filters. The 2 polyester filters examined combined good clinical and laboratory results with superior flow properties.

Adolescent↗
PubMed1990

Treatment of cerebral malaria by erythrocyte exchange.

A 33-years-old male presented with a severe malaria, caused by a chloroquine resistant strain of Plasmodium falciparum. The number of parasitized erythrocytes reached 20% and the patient had cerebral complication. During the second hospital day, an erythrocyte exchange was performed as an in addition to chemotherapy. The patient's clinical condition improved and the parasitemia disappeared. The erythrocyte exchange is recommended in severe malaria, when parasitemia greater than 10%, with or without cerebral, renal or blood coagulation complications.

Acute Disease↗
PubMed1985

[Antiglobulin test using an immunoenzyme method].

An enzyme-linked antiglobulin test (ELAT) is described. All reagents are commercially available. The antiglobulin reagent is a monoclonal anti-IgG conjugated with alkaline phosphatase. The substrate (p-nitrophenylphosphate) is stable and innocuous. The procedure requires long incubations, particularly between red cells and the enzyme-conjugated antibody. However most of the time actively spent by the technician is consumed by washing procedures (LAV). These may be avoided by using density gradient centrifugation (CSG). ELAT is significantly more sensitive than agglutination, using either washing or gradient centrifugation (P = 0.002 and P = 0.0005, respectively). Moreover ELAT-CSG is significantly more sensitive than ELAT-LAV (P = 0.03). CSG yields better reproducibility and probably avoids some of the spontaneous elution of the primary and/or secondary antibody which may occur during washing.

Alkaline Phosphatase↗
PubMed1983

[Hematological variables in marathon and ultramarathon runners].

The haemoglobin concentration of runners has been reported to be often below normal (1). In the present investigation the haemoglobin and iron concentrations and the haematocrit have been determined in 45 marathon runners examined before and after the 1982 Italian Marathon Championship and in 79 runners examined before and after the 1982 Firenze-Faenza race (107 km). The results obtained suggest that the training programs followed by the marathon runners (up to 260 km per week in the months preceding the race) are accompanied by a significant decrease of the haemoglobin and iron levels and of the haematocrit. Similar results have been observed in the participants to the Firenze-Faenza race. Nevertheless in these ultramarathoners the decrease of the haemoglobin and iron concentration and of the haematocrit are less marked, possibly because their training programs are less intense than those of the marathon runners. The findings obtained are in favour of the hypothesis that the degree of "sport anaemia" might be related to the amount of training of the athletes.

Adult↗
PubMedDate not supplied

Prevention of non-hemolytic transfusion reactions with leucocyte-poor blood: a prospective study.

Twenty-five patients with homozygous beta-thalassemia, who complained of occasional nonhemolytic transfusion reactions (NHTR), were alternately transfused eight times with normal packed red cells (PRC) and leucocyte-poor blood prepared by three methods: cotton wool filtration (CWF), buffy coat removal after dilution with saline and upright spin (BCR), and microaggregate filtration (MAF). On overall, CWF-treated RBC concentrates (CWF-RBC) contained 0.02 x 10(9) leucocytes; BCR-RBC contained 0.4 x 10(9), MAF-RBC 1.3 x 10(9) and PRC 1.7 x 10(9). The frequencies of NHTR were: CWF, 0.02; BCR, 0.04; MAF, 0.20; PRC, 0.28. Compared to PRC, both CWF and BCR significantly reduced the frequency of NHTR (p less than 0.01), whilst MAF did not (p greater than 0.1). On the other hand, in one patient CWF caused the two most severe NHTR recorded in this study, both of which were characterized by anaphylaxis.

Adolescent↗