[Morphology and morphogenesis of bovine syncytial virus--electron microscopy of ultramicrotomic sections].
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Biomedical subjects
Publications and source records attributed to R Riebe.
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Multilateral economic as well as techno-scientific cooperation is in a process of persistent expansion between the member countries of CMEA. This is the background against which the need for as well as the programme and the scientific, political, and economic importance of a reference centre for permanent cell lines (cell bank) at CMEA level are described. The centre is attached to Friedrich-Loeffler-Institut für Tierseuchenforschung which is run under the umbrella of the Academy of Agricultural Sciences of the GDR.
Titration of SVDV on primary pig kidney cell cultures revealed a plating efficiency of less than or equal to 0,9 X 10(-3). Concentration and purification of the SVD-Virus propagated on pig kidney cell cultures were done by chloroform treatment, adsorption, differential- and density gradient centrifugation. The following physical parameters were found: SVDV is an isometrical RNA-virus having a diameter of 25,1 +/- 1,0 nm. It is resistent to the action of chloroform, ether and pH. The virus has a sedimentation coefficient of 156 +/- 3S and a bouyant density in CsCl of 1,33 +/- 0,01 g/ml. Within the family of picornaviruses the SVDV belongs to the subgroup of enteroviruses and can be distinguished from the foot-and-mouth disease virus by the difference in pH-sensitivity and bouyant density in CsCl.