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Biomedical subjects

R S Galen

Publications and source records attributed to R S Galen.

At least 19 recordsLinked to original sources
PubMed1979

Evaluation of a nephelometric assay for haptoglobin and its clinical usefulness.

Serum haptoglobin has been advocated as an indicator of intravascular hemolysis. We have evaluated a nephelometric determination of serum haptoglobin. The assay is sensitive and exhibits within-run precision in the range of 2.5-7.4% coefficient of variation (CV) and between-run precision of 7.0% (CV). In addition, when haptoglobin values determined with the nephelometric assay were compared with hemoglobin-binding capacity determined by electrophoresis, the correlation coefficient was 0.968. The assay is essentially independent of phenotype and free of significant interference by hemolysis. The clinical correlation of haptoglobin values obtained for 100 selected patients with the nephelometric technique correlated well, if less than 250 mg/L, with the presence of hemolytic disease.

Anemia, Hemolytic↗
PubMed1978

Electrophoretic identification of the brain isoenzyme of creatine kinase following treatment with anti-BB antisera.

A procedure is described for accurately determining the presence of true creatine kinase isoenzyme BB by fluorescent staining following electrophoresis on either cellulose acetate or agarose. Treatment of sera with anti-BB inhibiting antibody prior to electrophoresis and subsequent enzymatic staining allows clear identification of creatine kinase BB in presence of interfering artifacts. The utilization of an immunological means of identifying BB following electrophoresis avoids the pitfall of associating an artifactual BB isoenzyme with a disease state. Using this technique, presence of creatine kinase BB in patients with carcinoma, cardiac disease and renal failure has been verified.

Antibodies↗
PubMed1977

Enzyme immunoassay of serum thyroxine with the "Autochemist" multichannel analyzer.

We have modified the homogeneous enzyme immunoassay of thyroxine and have adapted it to the AutoChemist. Both enzyme immunoassay and radioimmunnoassay procedures were performed on 2016 patients' sera on which thyroxine determinations had been ordered. We find the automated enzyme immunoassay to be precise and accurate, and for the first time it permits total automation of an immunoassay on a multichannel biochemical-profiling instrument. More importantly, it represents the first time that a thyroid-function test has been included in a multitest biochemical screening profile performed on a single automated instrument.

Autoanalysis↗
PubMed1977

The normal range: a concept in transition.

One of the most frequent and perhaps frustrating questions the pathologist is called on to answer regarding laboratory tests is, "What is normal?" In recent years the term "normal range" has been replaced by "reference range." "Upper limit of normal" has been replaced by "referent value," "critical value," or "cut-off point." Our literature now reflects increasing contributions from biostatistics, decision theory, and systems analysis. Yet, for most of us, although the names have changed, the problem has not. Understanding Bayes' Theorum will not help the busy internist when he complains about false-positive elevations in alkaline phosphatase activity on hospital admission profiles. The laboratory director must be able to integrate reference range data and predictive value theory in his own environment if he wishes to provide meaningful information to clinicians. It is the objective of this review to focus on the definition and integration of these areas of emerging concern for the laboratorian.

Age Factors↗
PubMed1975

Diagnosis of acute myocardial infarction. Relative efficiency of serum enzyme and isoenzyme measurements.

Total serum enzyme activity for creatine phosphokinase (CPK), alpha-hydroxybutyric dehydrogenase (HBDH), lactic dehydrogenase (LDH), and serum glutamic oxaloacetic transaminase (SGOT), as well as the isoenzymes of CPK and LDH, were measured on admission and for ten subsequent days in 100 patients admitted consecutively to a coronary care unit. On discharge, patients were classified by a cardiologist as either having or not having suffered an acute myocardial infarction (MI) on the basis of clinical and electrocardiographic criteria--without knowledge of the enzyme studies. The combined use of CPK and LDH isoenzyme levels provided the greatest laboratory discrimination between the two clinical groups (MI vs non-MI). The routine use of HBDH and SGOT levels can be abandoned in the setting of a coronary care unit if CPK and LDH isoenzyme assays are available.

Acute Disease↗