Bacteriology swabs during appendectomy: sample sites and transport media.
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Biomedical subjects
Publications and source records attributed to R S Miles.
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A prospective study was established to determine whether, using suitable transport media, bacteria could be isolated from the lesions of mammary duct ectasia. The results indicate that both aerobic and anaerobic organisms are present in a high proportion of patients with nipple discharge associated with this condition and in all patients who develop peri-areolar sepsis (abscess and mammillary fistulae) as part of the syndrome. The lesions of duct ectasia are therefore not sterile and the possibility exists that bacteria have a role in the aetiology and pathogenesis of this condition.
The results were compared of submitting simple swabs, swabs in Stuart's Transport Medium (STM) and swabs in Robertson's cooked-meat broth (RCMB), from 100 potentially or definitely infected sites in patients undergoing general surgery. Significantly more positive bacterial cultures were obtained from swabs sent in RCMB (65), than from swabs sent either in STM (39) or as simple swabs (32). The isolation of potentially significant organisms from only the RCMB series could influence clinical management. The conventional reluctance of bacteriologists to accept evidence obtained from RCMB cultures seeded directly in the ward or at operation is challenged.
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Faecal samples were collected from impounded stray dogs, classified as under or over six months of age, on their arrival in kennels and again 5-7 days later. The faeces were examined for the presence of campylobacters, salmonella and Giardia lamblia. No significant difference was found in the rate of isolation of these organisms between the two age groups. There was, however, a significant increase in the rate of isolation at days 5-7 compared with day 1. It is suggested that while dogs may acquire campylobacter infection in kennels, excretion of the organism may be intermittent and may be precipitated by stress. A low incidence of salmonellae and Giardia lamblia excretion was detected.
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A pilot study showed that following urinary tract endoscopy in out-patients there is a significant incidence of subsequent rigor and general practitioner call-out. A double-blind study was carried out in 690 consecutive patients to assess the effectiveness of prophylactic doses (1 g) of cephazolin, given intravenously or intramuscularly at the time of the procedure, compared with no treatmemnt or placebo. The results, assessed from replies to a questionnaire, showed that whereas cephazolin given intravenously did not affect the incidence of subsequent rigor there was a significant reduction compared with controls when the antibiotic was given in the same dose intramuscularly.
Out of 100 strains of meningococci examined 62 were resistant to 1 mg/l or more of sodium sulphadiazine, including 82% of group W135 strains and 69% of group B strains. All strains were sensitive in vitro to 0.5 mg/l of sodium fusidate. Sodium fusidate should be considered as a chemoprophylactic agent for those who may be at risk of meningococcal disease.
Significant bacteriuria (viable count greater than or equal to 10(5)/ml) was found on single testing in 10% of 400 consecutive diabetic women aged 15-65 yr routinely attending a diabetic clinic. Twenty patients with and 21 without significant bacteriuria were then randomly selected for measurement of cardiovascular autonomic reflexes, bladder ultrasound, and mictiography. There were no significant differences between these two groups in age, duration and type of diabetes, glycosylated hemoglobin, blood urea, and creatinine. Abnormalities of cardiovascular autonomic function were more common in the bacteriuric group (P less than .01). Although the bacteriuric group voided a smaller volume of urine, none had evidence of increased residual bladder volume of urine on ultrasound. Diabetic women with cardiovascular autonomic neuropathy appear to be at increased risk of developing bacteriuria. However, age is probably the most important factor influencing the prevalence of bacteriuria in diabetic and in nondiabetic women.