The highest sensitivity of the absorption-elution test for detection of A, B, and H antigens in human excretion traces was observed when the excretion traces were treated with protease C and lidase (hyaluronidase) and standard erythrocytes for analysis of the resultant eluates were treated with protease C. Cross-reactions of sera with antigens of contralateral specificity were ruled out by simple dilution of the sera; the reliability of antigen detection was not lost due to pronounced increase in the sensitivity of modified absorption-elution test.

ABO Blood-Group System↗

PubMedDate not supplied

[Status and prospects for developing forensic medical expertise on objects of biological origin].

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Evaluation Studies as Topic↗

PubMedDate not supplied

[Modern concepts of the structure and function of erythrocyte antigens (review of the literature on the 100th anniversary of discovering blood groups)].

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Blood Group Antigens↗

PubMedDate not supplied

[Serological detection of Rhesus antigen D in blood traces from Rh-negative (cde) people].

A D-like structure was serologically detected on the internal side of Rh-negative (cde) erythrocyte membrane. This structure is absolutely inaccessible for anti-D antibodies in morphologically intact liquid blood erythrocytes. In hemolyzed erythrocytes of blood stains this antigenic structure, serologically identified as Rh antigen D, is accessible for binding with anti-D antibodies and for detection by the absorption-elution test, particularly so after blood stain treatment with proteases.

Autoantibodies↗

PubMedDate not supplied

[Kell system: current ideas about structure, function, antigen nomenclature, methods of detection, and forensic-medical significance].

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Antigens↗

PubMedDate not supplied

[The investigation of blood and secretion stains by the absorption-elution reaction using anti-H monoclonal antibodies].

Possible use of monoclonal antibodies anti-H in absorption-elution reaction was studied. Blood and secretion stains on gauze were analysed. Practical usefulness of monoclonal antibodies anti-H for investigation of human blood and secretions was stated. Differences in interaction of monoclonal antibodies with traces of different origin were found.

ABO Blood-Group System↗

PubMedDate not supplied

[An ultramicromethod for determining rhesus antigens in liquid blood].

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Agglutination Tests↗

PubMedDate not supplied

[The protease method of detecting rhesus antigens in liquid blood studies for forensic medicine purposes].

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Acremonium↗