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R S Samoĭlova

Publications and source records attributed to R S Samoĭlova.

At least 19 recordsLinked to original sources
PubMed1995

[Errors in the diagnosis and therapy of chronic lympholeukemias].

The analysis of 67 cases of benign, progressive, tumor CLL, lymph node lymphocytoma, CLL sarcoma transformation showed that the cases were misdiagnosed in 35.8% cases. The most common mistake (25.4%) was aggressive chemotherapy based on histological diagnosis of prolymphocytic, prolymphocytic-lymphoblastic lymphosarcoma without consideration of cytological and clinical evidence, tumor phenotype. The authors think valid to use the following criteria in diagnosis of lymphoproliferative diseases: histological findings, clinical manifestations and blood picture, tumor cell cytology, primary location and predominant dissemination of the tumor, immunophenotype, characteristic chromosomal disorders, response to treatment.

Adult↗
PubMed1993

[B-cell immunity].

Explore the source record for details and available documents.

Antibody Formation↗
PubMed1993

[Proteolytic enzymes in human lymphocytic leukemia cells. I. Activity of dipeptidylaminopeptidase IV, plasminogen activator and cathepsins B and L in cells with different immunologic phenotype].

Immunological and biochemical study of lymphoid cells obtained from 20 patients with various forms of lymphoproliferative disorders has been carried out. It was found that different phenotypes of lymphoid cells at various stages of differentiation have different activity levels of dipeptidyl aminopeptidase IV (DAP IV), plasminogen activator (urokinase type) and cathepsins B + L. The highest proteinase activity values were found in the lysates of just those leukemic T-cells whose phenotypes corresponded to the initial stages of thymic differentiation or to activated T-cells. The 10 times lowered activity was found in the cell phenotypes of mature T-helpers and T-suppressors, and the activity of the both was at virtually the same level. In lymphoid cells of the B-lineage (from pre-B to mature B-lymphocytes) the proteinase activities did not differ essentially: they were 2 to 3 times lower than in the lymphoid progenitors. It was suggested that the regulated activity changes in some proteinases occur during differentiation along the T- or B-pathways. It is likely that the increases in DAP IV and cathepsins B + L activities are associated with the activation of mature lymphoid T- and B-cells. No direct correlation was found between the activity of either proteinase and the expression of any of the surface markers under study.

B-Lymphocytes↗
PubMed1991

[The characteristics of the clinical course of lymphosarcomas in conformity with the morphological variants of the WHO and Working Formulation classifications].

Histological and cytological preparations of the lymph nodes, spleen, bone marrow and other tumors from 140 patients with different variants of lymphosarcomas were subjected to a comparative clinicomorphological analysis. The data obtained were correlated to the WHO classification and the working formulation of non-Hodgkin's lymphomas intended for clinical use. It has been found desirable that the working formulation may be used for predicting the disease course and elaboration of the programs of lymphosarcoma treatment.

Adolescent↗
PubMed1991

[Glycosidase activity in phenotypically different pathologic lymphoid cells, found at various stages of differentiation].

The activities of seven glycosidases (six lysosomal and one cytosolic) were determined in B- and T-lymphoid cells differing by immunological phenotypes and occurring at various differentiation stages. The cells were isolated from the circulating blood, bone marrow or spleens of patients with various forms of lymphoproliferative disorders. The glycosidase activities varied significantly depending on the phenotype. The highest activity of all glycosidases was observed in cells with a common lymphoid cell progenitor phenotype. In cells having the phenotype of mature T- and B-cells the glycosidase activities were comparatively low. The changes in all glycosidase activities depending on the phenotype and differentiation stage usually occurred in the same direction; however, the degree of elevation or decline of activities of individual glycosidases was different. The activities of N-acetyl-beta-D-hexosaminidase and alpha-D-mannosidase changed dramatically, whereas the changes in the activity of cytosolic neutral alpha-D-glucosidase were less apparent. These data suggest that lysosomal glycosidases play specific roles in lymphoid cell differentiation.

B-Lymphocytes↗
PubMed1989

[Dipeptidyl aminopeptidase-IV in lymphocytes of patients with lymphoproliferative diseases].

DAP-IV activity (Gly-Pro-MCA hydrolysis, pH 7.8) was found in lysates of peripheral blood lymphocytes of patients with T- and B-cell forms of malignant lymphoproliferative diseases. The highest DAP-IV activity was seen in the cells of patients with a rare variant of T-cell lymphocytic leukemia (T-CLL); these cells expressed simultaneously the antigens of T helpers and T suppressors (Th and Ts) (OKT4+ and OKT8+). The DAP-IV activity about ten times less was found in the pathological cells with a phenotype of mature Th (Sezary disease), as well as in the cells expressing antigens of both Ts and natural killers (a rare variant of T-CLL). The same activity was also found in Ts (T gamma-lymphocytosis). The data obtained show that the differences in DAP-IV expression are connected with the differentiation step rather than with the belonging to a particular subpopulation of T-cells. DAP-IV activity, which was somewhat lower than that of T-cells, was found in B-lymphocytes of patients with B-CLL, hair-cellular leukemia, and non-Hodgkin's lymphoma. No correlation of DAP-IV activity with the level of E-cellular differentiation was observed.

Antigens↗
PubMed1987

[Morphometric characteristics of argentaffin nucleolar structures in peripheral blood lymphoid cells from patients with non-Hodgkin's lymphomas and chronic lymphoid leukemias].

Interphase lymphoid nucleoli of Ag-NOR stained site areas were measured on the TAS system in peripheral blood lymphoid cells with the known immunologic phenotype of 19 patients with lymphoid neoplasia (non-Hidgkin's lymphoma, CLL, hair-cell leukemia) and 10 healthy donors. The area of Ag-granules in neoplastic cells is much greater than in normal lymphocytes and does not correlate with a proliferation of cells but correlates with a malignancy grade of the disease. These data are discussed in terms of rDNA transcription patterns in malignant cells.

Adolescent↗
PubMed1987

[Preparation of a lymphoblastoid B-cell line from a long-term suspension culture of human bone marrow].

Lymphoblastoid cell line was obtained from long-term human bone marrow culture. The cell line was characterized using methods of immunological phenotyping, cytochemistry and cytogenetics. This cell line represents different stages of B-cell differentiation, karyotype 46 XX. The cells of this line were able to support their growth during more than 10 months without exogenous stimulation.

B-Lymphocytes↗
PubMed1986

[Diagnosis of reactive lymphadenopathies and the stage of lymphosarcoma leukemization using the simplest methods of immunologic phenotyping].

The authors have summarized the research data on lymphoid cells of the lymph nodes and peripheral blood of normal donors, patients with hematological nonlymphoproliferative diseases, reactive lymphadenopathies and malignant lymphoproliferative diseases (Hodgkin's disease, chronic lympholeukemia and lymphosarcomas). The data indicate that the use of the panel of the simplest tests of immunological phenotyping provides in many cases sufficient information for differential diagnosis of reactive lymphadenopathies and malignant lymphoproliferative diseases as well as for diagnosing the early stage of lymphosarcoma leukemization.

B-Lymphocytes↗