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Biomedical subjects

R S Samoilova

Publications and source records attributed to R S Samoilova.

8 recordsLinked to original sources
PubMed1997

The relevance of cytological studies and Ki-67 reactivity to the clinical course of chronic lymphocytic leukemia.

The aim of the study was to investigate whether the growth fraction and cytology of peripheral blood and lymphoid tissues relate to response to treatment in CLL. Growth fraction was assessed using the monoclonal antibody Ki-67. Peripheral blood (PB) films and lymph node and spleen touch imprints or aspirates were examined in 35 patients (pts) with CLL: smouldering CLL (4 pts) (no therapy), progressive CLL but responsive to chlorambucil and cyclophosphamide (15 pts) and resistant CLL (16 pts). Cytological studies of lymphoid tissues showed that pts with resistant CLL had an increased proportion of prolymphocytes and blasts and the highest Ki-67 expression (mean 6,6%). PB morphology was not different between the groups of resistant and responsive CLL. However, the cases with resistant CLL showed a higher percentage of Ki-67 positive PB cells (p<0,05). Four pts with indolent CLL had typical CLL morphology in the PB and the lowest numbers of prolymphocytes and blasts with Ki-67 positive cells in lymphoid tissues.

Adult↗
PubMed1990

[Possibilities of differential diagnosis of B-cell chronic lymphoproliferative diseases using monoclonal antibodies].

Immunological phenotyping of lymphoid cells of the peripheral blood and, in some cases, of the lymph nodes and spleen was conducted in 41 patients with B-cell chronic lymphoproliferative diseases. Four monoclonal antibodies (MCA) were used in the immunofluorescence test, the results of which were analyzed by means of microscopy and flow cytofluorometry as well as by rosette-formation with mouse red blood cells. Varying expression degree has been shown by two B-cell differentiation antigens identifiable with MCA CD24 and CD20 on lymphoid cells of patients with typical B-cell chronic lymphoid leukemia (B-CLL) and lymphosarcoma. The varying expression degree is characteristic of small lymphocytes and transformed lymphoid cells in both the diseases. Differences in the number of cells expressing CD 5 (T1) antigen in B-CLL and lymphosarcoma, as well as correlation of this marker with EM-receptor expression have been recorded. The possibilities of using the results of the study for the differential diagnosis of chronic B-cell lymphoproliferative diseases, including B-CLL and B-lymphocytic lymphoma, and for investigation of B-cell lymphoproliferative disease pathogenesis have been discussed.

Antibodies, Monoclonal↗
PubMed1978

Quantitative determination of human leukocyte interferon by microfluorometric immunoassay with FITC-labeled anti-interferon immunoglobulin.

The possibility of quantitative determination of human leukocyte interferon using FITC-labeled anti-interferon antibody was studied. Anti-interferon immunoglobulin of high specific activity was obtained as a result of long-term immunization of a donkey with human leukocyte interferon preparation and subsequent fractionation and immunoadsorption steps. This immunoglobulin was labeled with FITC and then used for titration of human leukocyte interferon by the fluorescence inhibition immunoassay. The titres of different preparations of human leukocyte interferon in the immunoassay were comparable with the titres of the same preparations detected by the inhibition of a cytopathic effect of VSV.

Animals↗
PubMed1978

Immunologic study of T and B lymphocytes in thymectomized monkeys after irradiation and autologous bone marrow transplantation.

Rhesus monkeys were exposed to total-body gamma-irradiation (60Co) with 700R following adult thymectomy, and then protected with autologous bone marrow. Transplantation immunity in TxIBM animals was impaired: skin allografts survived for 19 to 38 days. The percentage of T and B lymphocytes studied by the rosette technique and immunofluorescent staining for Ig did not substantially change when examined in early as well as late periods after TxIBM. Functional capacities of lymphocytes were measured in vitro by stimulation with phytohaemagglutinin and with allogeneic cells in mixed lymphocyte cultures. MLC reactivity in TxIBM monkeys did not recover in the early and later periods following treatment. PHA responsiveness was retained in both these periods. These findings together with the results obtained in the analysis of unstable chromosomal aberrations suggested that PHA-responsive and MLC-reactive cell subpopulations of monkey blood lymphocytes differed in the degree of thymus dependence.

Animals↗
PubMed1978

Transplantation of allogeneic bone marrow from thymus-deprived monkeys and certain characteristics of "graft-versus-host" cells.

Transplantation of allogeneic bone marrow both from normal and thymus-deprived donors (thymectomized lethally irradiated and restored with autologous bone marrow) was followed 6--7 days later by the appearance of large numbers of GVH cells in bone marrow and peripheral blood which were one of the characteristic features of acute GVHR. GVH cells isolated from peripheral blood 7 days after transplantation showed a high labelling index (about 30%) after 40 min incubation with 3H-thymidine. The prolongation of incubation failed to increase the proportion of labelled cells due to the low viability of GVH cells. The results obtained with T and B cell tests suggested that GVH cells had the properties of T lymphocytes: about 60% of the cells could form rosettes with non-sensitized sheep red blood cells; there were virtually neither Ig-bearing cells nor cells with receptors for C3 and Fc portion of Ig.

Animals↗
PubMed1975

Immunoglobulins on the surface of blast cells in human acute leukemia.

By means of the direct fluorescent antibody technique it was shown that malignant cells in acute lymphoblastic leukemia do not, as a rule, bear immunoglobulins on the cell surgace (43 of 45 cases). During complete remission the number of Ig-positive cells corresponds to the number of Ig-positive cells in normal lymphocyte populations. During incomplete remission as well as during incomplete remission as well as during intensive chemotherapy, the lymphocyte-like cells are evidently malignant, since they do not contain a normal number of Ig-positive lymphocytes. In acute myeloblastic leukemia (AML) and acute myelomonoblastic leukemia (AMML), lymphocytes distribute normally according to the number of Ig-positive and Ig-negative in 34 of 38 cases. AMML blast cells were Ig-positive in nine of ten cases. The data are discussed in the light of current concepts about the presence of a common precursor cell of granulocytes and monocytes.

Absorption↗