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Biomedical subjects

R S Shaw

Publications and source records attributed to R S Shaw.

10 recordsLinked to original sources
PubMed1974

An electron-capture gas-liquid-chromatographic method for the determination of prostaglandin F2alpha in biological fluids.

A sensitive electron-capture gas-liquid-chromatographic method for the determination of sub-nanogram quantities of prostaglandin F(2alpha) was developed. The method is based on the sub-microgram scale conversion of the prostaglandin into the electron-capturing pentafluorobenzyl ester, and analysis of the latter as the tris-trimethylsilyl ether. The lower limit of detection was 12.5pg of the ester injected ;on-column' as the silylated product. The method was successfully applied to the determination of prostaglandin F(2alpha) in monkey plasma. The specificity of the analytical procedure was increased by incorporating a thin-layer chromatographic fractionation before gas-liquid chromatography. The utility of the analytical methodology developed was demonstrated by its application to the determination of plasma concentrations of intact prostaglandin F(2alpha) in a Rhesus monkey, after subcutaneous administration of a single dose of prostaglandin F(2alpha). The electron-capture gas-liquid-chromatographic assay is compared with the radioimmunoassay and the gas-liquid-chromatographic-mass-spectrometry assay for the determination of prostaglandin F(2alpha).

Animals↗
PubMedDate not supplied

Non-medical drug use behaviour at five United States universities: a 15-year study.

A survey carried out in 1970, 1973, 1976, 1980 and 1984 by means of a questionnaire at five American universities, which involved a total of 4,171 students, showed an increase in the use of cocaine, cannabis, hallucinogens, sedatives, amphetamines and alcohol. Cocaine use increased from 2.7 per cent in 1970 to 30 per cent in 1984, while cannabis use almost doubled during that period. The survey found that intercollegiate athletes used significantly more anabolic steroids than non-athletes, but with regard to the use of other substances the athletic students did not differ significantly from non-athletic students.

Adolescent↗