Biomedical subjects

R Süssmuth

Publications and source records attributed to R Süssmuth.

57 records · Page 4Linked to original sources

PubMedDate not supplied

Swarming phenomena, basis of a method to identify motile bacteria densitometrically.

The swarming phenomenon of 18 strains was tested with the intention to identify motile, swarming bacteria. Not all bacteria which are motile in liquid are able to swarm on semisolid nutrient broth, but all swarming bacteria show characteristic swarming developments, resulting in different swarming zones which could be scanned densitometrically. The densitograms are fully reproducible for the same strain and show similarities between strains of one species.

Bacteria↗

PubMedDate not supplied

[Protein synthesis with cell extracts of Micrococcus radiodurans (author's transl)].

Pure active ribosomes of cells of Micrococcus radiodurans could be obtained when cultivated in trypton, glucose and nutrient broth by adding natrium citrate. The optimal conditions for a cell-free protein synthesis were investigated at the (polyuridylic acid) dependent polyphenylalanine synthesis. When exchanging ribosomes and S100-fractions with the corresponding fractions of E. coli, we found that the enzyme fractions of M. radiodurans extremely inhibit the ribosomal activity. The incorporation rates in the cell-free system of M. radiodurans yield, at comparable conditions, in relation to E. coli under 10%.

Bacterial Proteins↗

PubMedDate not supplied

Prophage induction by alkylating ethyl methylaminosulfonate.

Treatment of the lysogenic strain M. lysodeikticus 53-40 (N5) with ethyl methylaminosulfonate results in a slight increase in infective center concentration of N5 phages which is only detectable once disturbing cells had been eliminated by lysozyme. After conversion of ethyl methylaminosulfonate with radioactive labelled guanosine at 37 degrees C, N7-ethyl-guanine could be ascertained. Therefore the biological activity of the drug may be due to an ethylation of DNA.

Alkylation↗