PROPERTIES OF RIBOSOMES AND RNA FROM ASPERGILLUS NIGER.
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Biomedical subjects
Publications and source records attributed to R STORCK.
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Dormant ascospores contain the same ribosomal population as growing hyphae and resting conidia. This conclusion is based on analyses of sedimentation coefficients and of nucleotide composition of ribosomes, ribosomal RNA, and soluble RNA. Thus dormancy is not characterized by the absence of the important parts of the protein-synthesizing apparatus.
Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20 degrees C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl(2) but undergo a dissociation into smaller particles if the MgCl(2) concentration is lowered to 0.0001 M. Two types of RNA with sedimentation coefficients of 19S(20) (50) and 13S(20) (50) have been extracted from the 81S particles.
Henney, H. (The University of Texas, Austin) and R. Storck. Nucleotide composition of ribonucleic acid from Neurospora crassa. J. Bacteriol. 85:822-826. 1963.-The nucleotide composition of total, ribosomal, and soluble ribonucleic acid (RNA) from the conidia and the hyphae of Neurospora crassa was determined. The corresponding RNA classes from the two morphological types had the same base ratio. Total, ribosomal, and soluble RNA from hyphae contained, respectively, 51.0, 50.2, and 61.6% guanylic acid (G) plus cytidylic acid (C), and from conidia, 50.6, 49.9, and 62.1%. The proportion of nucleotides in ribosomal RNA was in close agreement with that reported for ribosomal RNA from other organisms. Soluble RNA contained 2.8% pseudouridylic acid, 36.0% C, and 25.5% G, and differed from soluble RNA from other sources in its high C content. Centrifugation of purified RNA from conidia and hyphae, through a linear sucrose-density gradient, yielded identical sedimentation profiles.
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