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Biomedical subjects

R Sakazaki

Publications and source records attributed to R Sakazaki.

At least 19 recordsLinked to original sources
PubMed1992

[Typing of methicillin-resistant Staphylococcus aureus by antibiotic resistance phenotypes as an epidemiological marker in nosocomial infections].

A total of 129 clinical isolates of methicillin-resistant Staphylococcus aureus was tested for the susceptibility to 28 antibiotics, antiseptics and heavy metal salts, according to the method of Gillespie et al. The strains showed resistance ranging from 20% to 80%, to 10 agents, such as amikacin, chloramphenicol, clindamycin, gentamicin, neomycin, streptomycin, tobramycin, acriflavine, cadmium nitrate, ethidium bromide. These agents were finally chosen for typing in this study. Strains resistant to 10 agents were distributed into 55 phenotypes. Most strains isolated from one ward belonged to the specified phenotype, whereas strains isolated from other wards were divided into a variety of phenotypes. It seemed to be the occurrence of nosocomial infection in this hospital. This typing method gives the merit to be easy, economical, rapid and reproducible for the epidemiological investigations in the clinical bacteriological laboratory.

Anti-Bacterial Agents↗
PubMed1991

Biochemical and genetic characterization of autoagglutinating phenotypes of Aeromonas species associated with invasive and noninvasive disease.

The genetic characteristics and biochemical and structural properties of a number of autoagglutinating (AA) strains of Aeromonas associated with invasive and noninvasive disease in humans and infections in animals and from environmental sources were investigated. Of 27 strains analyzed by multilocus enzyme typing and DNA hybridization studies, 25 (93%) were confirmed to belong to either hybridization group 1 (phenospecies and genospecies Aeromonas hydrophila) or 8 (phenospecies Aeromonas sobria; genospecies Aeromonas veronii). Further analysis of 19 of these strains indicated that four major groups could be identified on the basis of serologic and surface characteristics, protein and lipopolysaccharide composition, and virulence properties; these groupings held true regardless of the site of isolation or disease process involved. The major AA+ group identified was serogroup O:11, whose strains possessed an S layer, were resistant to the bactericidal activity of normal serum, and were pathogenic in mice. The results suggest a set of useful phenotypic and structural markers for identification of specific subsets of mesophilic Aeromonas involved in a wide range of infections in the animal kingdom.

Aeromonas↗
PubMed1990

Two serogroups of Vibrio cholerae non-O1 possessing somatic antigen factors in common with Hakata serogroup of V. cholerae.

Two serogroups of Vibrio cholerae non-O1 possessing somatic antigen factors in common with Hakata serogroup of V. cholerae are described. One group was included in the Hakata serogroup and has its own antigen other than all O-antigens of the Hakata group. The other group had its own major antigen, but not factor F specific for the Hakata group.

Agglutination Tests↗
PubMed1979

The structures of tridecaptins B and C (studies on antibiotics from the genus Bacillus. XXV).

On examining the structures of the antibiotics tridecaptins B and C, the constituent amino acids were separated and their chiralities were determined. The constituent fatty acids were identified by gas chromatography and mass spectrometry. Cleavage with N-bromosuccinimide and sequential analysis by EDMAN degradation demonstrates the sequence of the C-terminal side of tridecaptins B and C. Deacylation with polymyxin acylase of tridecaptin B and successive EDMAN degradation revealed the sequence of the N-terminal side of tridecaptin B. Finally partial acid hydrolysis on tridecaptin C clarified the sequence of the N-terminal side of tridecaptin C.

Amino Acids↗
PubMed1979

Numerical classification of Yersinia enterocolitica and relationship between its subdivision and pathogenicity.

A total of 102 strains of Yersinia enterocolitica were studied on their morphology, physiology and biochemistry at 25 degrees C. All data coded in binary notation and similarities between each pair of strains by simple matching coefficient were calculated using subsequent computation. Four clusters were distinguished within the 102 strains at a similarity level of 90--93%. Although some discrepancies were recognized on reactions of indole, xylose, esculin and salicin, it was evident that 3 of the 4 groups in the present study correspond to the DNA relatedness groups of Brenner et al., respectively. On the other hand, it was confirmed, using feeding tests with monkeys, that only the group 1 was enteropathogenic. These results support the suggestion of Brenner et al. that their DNA relatedness group 1 is true Y. enterocolitica.

Animals↗
PubMed1978

Two epidemics of diarrhoeal disease possibly caused by Plesiomonas shigelloides.

Two epidemics of water-borne diarrhoeal disease involving a total of 1000 persons are reported. In both epidemics, none of the usual bacterial entero-pathogens were recognized and Plesiomonas shigelloides was the only suspect aetiological agent isolated. The ecology of P. shigelloides was investigated in these outbreaks. It was recognized as an inhabitant of fresh surface water and its presence was closely related to warm weather.

Bacterial Infections↗
PubMed1978

Prevalence of non-cholera vibrios in cavum nasi and pharynx of ducks.

Investigations among ducks on ten different farms showed a high prevalence of Vibrio cholerae in cavum nasi and Pharynx after the ducks were admitted to the open field. In no case was Vibrio cholerae isolated from ducks which had never been outside the houses. At least six serovars were isolated, 0:54 being the most prevalent. All the strains isolated produced a distinctive cytotoxic effect in YI adrenal cells and caused fluid accumulation in rabbit gut loops. Migratory birds were incriminated as the source of initial contamination.

Age Factors↗
PubMed1978

On the serology of Plesiomonas shigelloides.

The serology of 87 strains of Plesiomonas shigelloides was studied. Thirty O antigenic groups and 11 H antigens were defined within the 87 strains, and an antigenic schema consisting of 40 serovars was established. Some O antigens of P. shigelloides were identical or closely related to those of some Shigella serovars.

Agglutination Tests↗